稻米淀粉理化特性相关的水稻淀粉分支酶基因标记开发

中国水稻科学

稻米淀粉理化特性相关的水稻淀粉分支酶基因标记开发

李刚^1,2,#,邓其明^1,2,#,万映秀³,李双成^1,2,肖勇^1,2,周鹏^1,2,王世全^1,2 ,李平^1,2,*

¹四川农业大学水稻研究所，四川温江 611130； ²四川农业大学西南作物基因资源与遗传改良教育部重点实验室，四川雅安 625014； ³安徽省农业科学院作物研究所，安徽合肥 230031； ^# 共同第一作者； *通讯联系人, E-mail: liping@cngk.com

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-11-10 发布日期:2008-11-10

^{Development of Molecular Markers for Sbe Gene in Association with Starch Characteristic in Rice}

LI Gang^1,2,#, DENG Qiming^1,2,#, WAN Yingxiu³, LI Shuang cheng^1,2,XIAO Yong^1,2, ZHOU Peng^1,2, WANG Shiquan^1,2, LI Ping1,2,*

¹Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; ²Key Laboratory of Southwest Crop Genetic Resource and Improvement, Ministry of Education, Sichuan Agricultural University, Ya′an 625014, China; ³Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China; ^# These authors contributed equally to this paper; *Corresponding author, E-mail: liping@cngk.com

Received:1900-01-01 Revised:1900-01-01 Online:2008-11-10 Published:2008-11-10

摘要/Abstract

摘要： 基于籼稻9311和粳稻日本晴基因组间的序列差异，成功发展了水稻淀粉分支酶基因(Sbe)9个分子标记。利用这些标记对102份非糯材料进行了基因型检测，并分析了Sbe1、Sbe3基因标记基因型多态性对稻米淀粉理化特性的影响。Sbe1、Sbe3基因标记多态性位点对揭示非糯材料间稻米淀粉理化特性的差异不显著，说明淀粉分支酶基因这些等位性变异位点对非糯稻米的蒸煮食味品质影响不显著；在鉴定籼稻品种时，有6个标记的鉴定准确率达80%以上。还对标记开发的意义和分子标记辅助选择育种进行了探讨。

关键词: 水稻, 淀粉分支酶基因, 分子标记, 淀粉黏滞性

Abstract: Based upon the genomic sequence difference between the indica rice variety 9311 and the japonica rice variety Nipponbare, a total of nine molecular markers for starch branching enzyme gene(Sbe) were successfully developed. All the markers were utilized for genotypic screening and further analysis of the correlation between genetic polymorphisms of Sbe1 and Sbe3 genes and the rice starch characteristics. The genetic polymorphism of the markers had no influence on starch physiochemical traits, that is these allelomorphism variation sites of Sbe gene had no influence on the starch traits of nonglutinous rice. There were six markers which could be used to accurately identify indica rice varieties with a reliability over 80%. Meaning of marker development and molecular marker assisted selection were also discussed.

Key words: rice, starch branching enzyme gene, molecular marker, starch viscosity, variety identification

李刚,邓其明,万映秀,李双成肖勇周鹏王世全李平. 稻米淀粉理化特性相关的水稻淀粉分支酶基因标记开发[J]. 中国水稻科学 2008,22(6): 564-564～570 . .

LI Gang,#,DENG Qiming,#,WAN Yingxiu,LI Shuang cheng,XIAO Yong,ZHOU Peng,WANG Shiquan,LI Ping .

^{Development of Molecular Markers for Sbe Gene in Association with Starch Characteristic in Rice}

[J]. Chinese Journal of Rice Science 2008,22(6): 564-564～570 ..

参考文献

[1]Bligh H F L, Till R I, Jones C A. A microsatellite sequence closely linked to the wx gene of Oryza sativa. Euphytica, 1995, 86: 83-85.
[2]Hirano H Y, Eiguchi M, Sano Y. A single base change altered the regulation of the waxy gene at the posttranscriptional level during the domestication of rice. Mol Biol Evol, 1998, 15: 978-987.
[3]Tan Y F,Zhang Q F. Correlation of simple sequence repeat (SSR) variants in the leader sequence of the wx gene with amylose content of the grain in rice. Acta Bot Sin, 2001, 43: 146-150.

[4]Ayres N M, McClung A M, Larkin P D, et al. Microsatellites and a singlenucleotide polymorphism differentiate apparent amylose classes in an extended pedigree of US rice germ plasm. Theor Appl Genet, 1997, 94: 773-781.
[5]Yamanouchi H, Nakamura Y. Organ specificity of isoforms of starch branching enzyme (Qenzyme) in rice. Plant Cell Physiol, 1992, 33: 985-991.
[6]Mizuno K, Kawasaki T, Shimada H, et al. Alteration of the structural properties of starch components by the lack of an isoform of starch branching enzyme in rice seeds. J Biol Chem, 1993, 268: 19084-19091.
[7]Takeda Y, Hizukuri S. Structures of amylopectins with low and high affinities for iodine. Carbohydr Res, 1987, 168: 79-88.
[8]Ong M H, Blanshard J M V. Texture determinants of cooked,parboiled rice:Ⅱ.Physicochemical properties and leaching behaviour of rice. J Cereal Sci, 1995, 21: 261-269.
[9]舒庆尧，吴殿星，夏英武，等. 稻米淀粉RVA谱特征与食用品质的关系. 中国农业科学， 1998, 31(3): 25-29.
[10]隋炯明，李欣，严松，等. 稻米淀粉RVA谱特征与品质性状相关性研究.中国农业科学, 2005, 38(4): 657-663.
[11]包劲松，何平，夏英武，等.稻米淀粉RVA谱特征主要受Wx基因控制. 科学通报， 1999, 44(18): 1973-1976.
[12] Bao J S, Zheng X W, Zhu L H, et al. QTL mapping for the viscosity characteristics in rice(Oryza sativa L.). Theor Appl Genet, 2000, 100: 280284.
[13]包劲松，朱立煌. 水稻Wx基因(CT)n微卫星标记与稻米淀粉品质的关系研究. 农业生物技术学报， 2000, 8(3): 241-244.
[14]万映秀，邓其明，李平，等. 水稻Wx基因的遗传多态性及其与主要米质指标的相关性分析. 中国水稻科学, 2006, 20(6): 603-609.
[15]Murray M G, Thompson W F. Rapid isolation of high molecular weight liant DNA. Nucl Acids Res, 1980, 8: 4321-4325.
[16] 王凤格，赵久然，郭景伦，等. 一种改进的玉米SSR标记的PAGE/快速银染检测新方法. 农业生物技术学报， 2004, 12(5): 606-607.
[17]唐启义，冯明光. 实用统计分析及其DPS数据处理系统. 北京：科学出版社, 2002.
[18] Xie D L, Wang Z Y, Hong M M. The evidence for the excision of TouristOs6 from the promoter region of the rice sbe1 gene. Acta Phytophy Sin, 1999, 25: 274-280.
[19]蔡星星，刘晶，仇吟秋，等. 籼稻9311和粳稻日本晴DNA插入缺失差异片段揭示的水稻籼粳分化. 复旦学报：自然科学版， 2006, 45（5）： 309-315.
[20]李浩杰，李平，高方远，等. SSR标记辅助选择改良冈46B直链淀粉含量的研究. 作物学报， 2004, 30(11): 1159-1163.
[21]蔡毅，谢冬绿，王宗阳，等. 水稻淀粉分支酶基因5′上游区缺失对基因表达的影响.植物生理与分子生物学学报， 2002, 28(1): 23-27.
[22]韩月澎. 稻米淀粉合成相关基因对品质的影响及分支酶基因Sbe1、Sbe3克隆与分析[学位论文]. 扬州：扬州大学， 2004.