粳稻SSR连锁图谱的构建及恢复系卷叶性状QTL分析

中国水稻科学

粳稻SSR连锁图谱的构建及恢复系卷叶性状QTL分析

郭媛¹, 程保山^1，2, 洪德林^1，*

¹南京农业大学作物遗传与种质创新国家重点实验室，江苏南京 210095； ²江苏徐淮地区淮阴农业科学研究所，江苏淮安 223001； * 通讯联系人， E-mail：delinhong@njau.edu.cn

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-10 发布日期:2009-05-10

Construction of SSR Linkage Map and Analysis of QTL for Rolled Leaf of Restorer Line in japonica Rice (Oryza sativa)

GUO Yuan¹, CHENG Baoshan ^1,2, HONG Delin^1,*

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095，China; ²Huaiyin Institute of Agricultural Sciences of Xuhuai Region of Jiangsu， Huai′an 223001; *Corresponding author, E-mail: delinhong@njau.edu.cn

Received:1900-01-01 Revised:1900-01-01 Online:2009-05-10 Published:2009-05-10

摘要/Abstract

摘要： 调查了粳稻品种秀水79 (P1)与粳稻恢复系C堡 (P2)及其衍生的254个重组自交系的SSR标记基因型和两个环境下主茎剑叶卷曲度，构建了该组合的SSR标记连锁图谱并分析了剑叶卷曲度QTL及其与环境的互作。在检测的818对SSR引物中，有90对引物在P1与P2之间扩增出多态性条带。单标记回归分析显示有12个标记在两个环境下均显示与剑叶卷曲度呈极显著相关。74个信息位点构成的连锁图谱全长744.6 cM，位点间平均图距10.1 cM。利用两种分析软件 WinQTLcart 2.5和QTLNetwork 2.0共同检测到3个QTL (qRL1、qRL7和qRL81)，其中qRL81是新发现的，在两个环境下贡献率分别为15.5%和12.8%，加性贡献率为6.6%，且与环境不存在互作。

关键词: 粳稻, 恢复系, 叶片卷曲度, 数量性状座位, 基因型与环境互作

Abstract: SSR marker genotypes and phenotypes of flag leaf rolling index (LRI) were investigated in Xiushui 79 (P1, a japonica variety), C Bao(P2, a japonica restorer line) and 254 recombinant inbred lines derived from the cross between Xiushui 79 and C Bao in japonica rice (Oryza sativa L.). A genetic map of the cross was constructed. QTLs for LRI were detected and their interaction with environments was analyzed. Among 818 pairs of SSR primers detected, 90 primers amplified polymorphic products between P1 and P2. Twelve markers showed highly significant correlations with LRI in both two environments based on single marker regression analysis. A genetic map containing 74 information loci was constructed, which covers a total distance of 744.6 cM, averaging 10.1 cM between two loci. Three QTLs (qRL1, qRL7 and qRL81) were detected with two analysis softwares WinQTLcart 2.5 and QTLNetwork 2.0. qRL81 was a new locus, accounting for 155% and 12.8% of phenotypic variation in the two environments, respectively. Phenotypic variation explained by additive effect was 6.6%. No interaction was found between qRL81 genotype and environments.

Key words: japonica rice, restorer line, leaf rolling index, quantitative trait locus, genotype and environment interaction

郭媛, 程保山洪德林,. 粳稻SSR连锁图谱的构建及恢复系卷叶性状QTL分析[J]. 中国水稻科学 2009,23(3): 245-251 . .

GUO Yuan,CHENG Baoshan ,HONG Delin. Construction of SSR Linkage Map and Analysis of QTL for Rolled Leaf of Restorer Line in japonica Rice (Oryza sativa) [J]. Chinese Journal of Rice Science 2009,23(3): 245-251 ..

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