水稻矮化突变体ddu1的遗传分析和分子定位

摘要/Abstract

摘要： 利用甲基磺酸乙酯（EMS）诱变粳稻品种兰胜获得了一个能稳定遗传的矮化突变体ddu1。GA3点滴诱导水稻第2叶叶鞘伸长和α淀粉酶诱导反应表明，ddu1并非为GA的缺陷型和信号传导阻碍矮化。将该突变体与籼稻浙辐802、明恢63和粳稻品种日本晴进行正反交配组，遗传分析表明该突变体受隐性单基因控制，而等位性检测表明ddu1与d1、d18、eui1和eui2均不等位。通过SSR和STS分子标记对F2代分离群体进行遗传定位，将该基因定位于第7染色体SSR标记RM427附近，随后又发展了多对有多态性的SSR和STS分子标记，最终将该基因定位于STS标记R5309和R3742之间，遗传距离分别为0.4和2.0 cM。

关键词: 水稻, 株高, 矮化基因, 分子标记, 遗传分析, 基因定位

Abstract: Abstract: A dwarf mutant ddu1 was originally identified from a japonica cultivar Lansheng by ethyl methanesulfonate（EMS） treatment. The elongation of the second leaf sheath in response to GA3 treatment and αamylase induction in embryoless half seeds suggested the dwarfism was not the mutation of GA biosynthesis and signal transduction. Reciprocal cross with Minghui 63, Zhefu 802 and Nipponbare were made to establish genetic population and genetic analysis showed that the mutant was controlled by a single recessive gene. Allelic test indicated that it was not allelic with d1, d18, eui1 and eui2. The marker RM427 linked to ddu1 was obtained by the SSR and STS molecular marker screening and the gene was finally located between the STS molecular markers R5309 and R3742 with the distances of 0.4 and 2.0 cM respectively in 475 kb on chromosome 7.

Key words: rice, plant height, dwarf gene, molecular marker, genetic analysis, gene mapping

胡江, 曾大力, 张光恒, 郭龙彪, 董国军, 高振宇, 胡兴明, 朱丽, 刘坚, 钱前. 水稻矮化突变体ddu1的遗传分析和分子定位[J]. 中国水稻科学 2009,23(3): 252-256 . .

HU Jiang,ZENG Dali,ZHANG Guangheng,GUO Longbiao,DONG Guojun,GAO Zhenyu,HU Xingming,ZHU Li,LIU Jian,QIAN Qian*. Genetic Analysis and Gene Mapping of a New Mutant of dwarf and disproportionate uppermostinternode1 (ddu1) in Rice
[J]. Chinese Journal of Rice Science 2009,23(3): 252-256 ..

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