Genetic Analysis and Gene Mapping of a New Mutant of dwarf and disproportionate uppermostinternode1 (ddu1) in Rice
HU Jiang, ZENG Dali, ZHANG Guangheng, GUO Longbiao, DONG Guojun, GAO Zhenyu, HU Xingming, ZHU Li, LIU Jian, QIAN Qian*
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; *Corresponding author, E-mail: Qianqian188@hotmail.com
Abstract：
Abstract: A dwarf mutant ddu1 was originally identified from a japonica cultivar Lansheng by ethyl methanesulfonate（EMS） treatment. The elongation of the second leaf sheath in response to GA3 treatment and αamylase induction in embryoless half seeds suggested the dwarfism was not the mutation of GA biosynthesis and signal transduction. Reciprocal cross with Minghui 63, Zhefu 802 and Nipponbare were made to establish genetic population and genetic analysis showed that the mutant was controlled by a single recessive gene. Allelic test indicated that it was not allelic with d1, d18, eui1 and eui2. The marker RM427 linked to ddu1 was obtained by the SSR and STS molecular marker screening and the gene was finally located between the STS molecular markers R5309 and R3742 with the distances of 0.4 and 2.0 cM respectively in 475 kb on chromosome 7.
HU Jiang,ZENG Dali,ZHANG Guangheng et al. Genetic Analysis and Gene Mapping of a New Mutant of dwarf and disproportionate uppermostinternode1 (ddu1) in Rice [J]. , 2009, 23(3): 252-256 .
[1]
Takahashi M, Takeda K. Type and grouping of internode pattern in rice culmgenetical studies on rice plant. J Fac Agric Hokkaido Univ, 1969, 7: 32-43.
[2]
Takeda K. Internode elongation and dwarfism in some gramineous plants. Gamma Field Symp, 1977, 16: 1-18.
[3]
Hong Z, UeguchiTanaka M, Umemura K, et al. A rice brassinosteroiddeficient mutant, Ebisu Dwarf (D2), is caused by loss of function of a new member of cytochrome P450. Plant Cell, 2003, 15: 2900-2910.
[4]
Tanabe S, Ashikari M, Fujioka S, et al. A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. Plant Cell, 2005, 17: 776-790.
[5]
Yamamuro C, Ihara Y, Wu X, et al. Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint. Plant Cell, 2000, 12: 1591-1605.
[6]
Mori M, Nomura T, Ooka H, et al. Isolation and characterization of a rice dwarf mutant with a defect in brassinosteroid biosynthesis. Plant Physiol, 2002, 130: 1152-1161.
[7]
Ashikari M, Wu J, Yano M, et al. Rice gibberellininsensitive dwarf mutant gene Dwarf 1 encodes the αsubunit of GTPbinding protein. Proc Natl Acad Sci USA, 1999, 96: 10284-10289.
[8]
Ishikawa S, Maekawa M, Arite T, et al. Suppression of tiller bud activity in tillering dwarf mutants of rice. Plant Cell Physiol, 2005, 46(1): 79-86.
[9]
Mitsunaga S, Tashiro T, Yamaguchi J. Identification and characterization of gibberellininsensitive mutants selected from among dwarf mutants of rice. Theor Appl Genet, 1994, 87: 705-712.
[10]
Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8(19): 4321-4325.
[11]
Lander E S, Green P, Abrahamson J, et al. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1(2): 174-181.
[12]
Michelmore R W, Papan I, Kesseli R V. Identification of markers linked to diseaseresistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828-9832.
[13]
Itoh H, UeguchiTanaka M, Sentoku N, et al. Cloning and functional analysis of two gibberellin 3βhydroxylase genes that are differently expressed during the growth of rice. Proc Natl Acad Sci USA, 2001, 98(15): 8909-8914.
[14]
Itoh H, Tatsumi T, Sakamoto T, et al. A rice semidwarf gene, TanGinbozu (D35), encodes the gibberellin biosynthesis enzyme, entkaurene oxidase. Plant Mol Biol, 2004, 54: 533-547.
[15]
UeguchiTanaka M, Ashikari M, Nakajima M, et al. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature, 2005, 437: 693-698.
[16]
Sasaki A,Itoh H, Gomi K, et al. Accumulation of phosphorylated repressor for gibberellin signaling in an Fbox mutant. Science, 2003, 299: 1896-1898.
[17]
Spiemyer W, Ellis M H, Chandler P M. Semidwarf (sd-1),“green revolution” rice, contains a defective gibberellin 20oxidase gene. Proc Natl Acad Sci USA, 2002, 99(13): 9043-9048.
[18]
Sasaki A, Ashikari M, UeguchiTanaka M, et al. A mutant gibberellin synthesis gene in rice. Nature, 2002, 416: 701-702.
[19]
Monna L, Kitazawa N, Yoshino R, et al. Positional cloning of rice semidwarfing gene, sd1: Rice “green revolution gene” encodes a mutant enzyme involved in gibberellin synthesis. DNA Res, 2002, 9(1): 11-17.
[20]
Sato Y, Sentoku N, Miura Y, et al. Lossoffunction mutations in the rice homeobox gene OSH15 affect the architecture of internodes resulting in dwarf plants. EMBO J, 1999, 18(4): 992-1002.
[21]
Ikeda A, UeguchiTanaka M, Sonoda Y, et al. Slender rice, a constitutive gibberellin response mutant, is caused by a null nutation of the SLR1 gene, an ortholog of the heightregulating gene GAI/RGA/RHT/D8. Plant Cell, 2001, 13: 999-1010.
Zhu Y Y, Nomura T, Xu Y H, et al. ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice. Plant Cell, 2006, 18: 442-456.
2009, Vol. 23 
