1 College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China; 2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 3Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; *Corresponding authors, E-mail: email@example.com; firstname.lastname@example.org
A spotted and yellow leaf mutant was isolated from more than 15 000 transgenic rice lines. The mutant was featured by lesionmimic spots during the whole growth stage as well as leaf etiolation one by one from lower part to upper part. Genetic analysis revealed that the mutation was controlled by a single recessive gene, which was designated as syl1 (spotted and yellow leaves 1). PCR analysis and hygromycin resistance assay showed that the mutation was not caused by TDNA insertion. To isolate the syl1 gene, a positional cloning strategy was employed. The approximate map position of the syl1 gene was determined by SSR markers from published data, and then nine new InDel markers were developed. A highresolution physical map of the chromosomal region around the syl1 gene was made using F2 and F3 populations consisting of 1328 mutant individuals. Finally, the syl1 gene was located within 102 kb region between InDel markers WL32 and WL35 within the BAC clone OSJNBa0002O20 on chromosome 12.
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