中国水稻科学
     首页  |  期刊介绍  |  编 委 会  |  投稿指南  |  期刊订阅  |  广告服务  |  留言板  |  联系我们  |  English
中国水稻科学  2011, Vol. 25 Issue (3): 249-255     DOI: 10.3969/j.issn.1001-7216.2011.03.004
研究报告 最新目录 | 下期目录 | 过刊浏览 | 高级检索  |   
双胚苗水稻单倍体及其杂交后代基因组DNA甲基化特异位点的分析及功能探讨
吴绍华;薛晶晶;张红宇;徐培洲;吴先军*
四川农业大学 水稻研究所/西南作物基因资源与遗传改良教育部重点实验室, 四川 成都611130; *通讯联系人, E-mail:wuxjsau@126.com
Analysis of Special DNA Methylated Sites Between Haploid of TwinSeedling and Its Hybrids in Rice
WU Shao-hua, XUE Jing-jing, ZHANG Hong-yu, XU Pei-zhou, WU Xian-jun*
Rice Research Institute/Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China; *Corresponding author, E-mail: wuxjsau@126.com
 全文: PDF (1231 KB)   HTML (1 KB)   输出: BibTeX | EndNote (RIS)      背景资料
摘要 采用甲基化敏感性扩增多态性技术对水稻单倍体SARⅡ628及它与蜀恢527、蜀恢363的杂交后代基因组DNA甲基化位点进行了分析。用16对选择性扩增引物在双亲及杂交后代中共检测到765个DNA甲基化位点,与双亲相比,杂交后代DNA甲基化水平均有不同程度的降低。通过分析两个杂交组合与双亲的甲基化带型差异,发现非单倍体遗传及单倍体独立遗传两种甲基化差异位点。对部分甲基化位点的序列进行功能分析,发现这些位点主要涉及细胞构造、代谢途径、应激反应等生物学功能。推测这类功能基因的甲基化修饰调控着相关基因的开启与关闭,为植物的生存、生长、发育及进化提供动力。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
吴绍华
薛晶晶
张红宇
徐培洲
吴先军
关键词双胚苗水稻   单倍体   甲基化敏感性扩增多态性   DNA甲基化     
AbstractThe DNA cytosine methylation mutation at the 5′CpCpGpG sites of the haploid of SARII628, and its hybrids with Shuhui 527, Shuhui 363 were analyzed by employing the MSAP (methylation sensitive amplification polyphisim) method. A total of 765 DNA methylated sites were detected and the methylation level of hybrids was lower than that of the parents. Meanwhile, different bands between hybrids and parents were analyzed and two types of methylated sites were detected, of which one inherited from the haploid, and the other didn′t. The biological functions of genes related to methylated sites involved in cell structure, metabolize and stress reaction. Therefore, DNA methylated modifications may be involved in the regulation of related genes, playing an important role in plant growth, development and envolution.
Key wordstwin-seedling rice   haploid   methylation sensitive amplification polymorphism   DNA methylation   
收稿日期: 1900-01-01;
引用本文:   
吴绍华,薛晶晶,张红宇等. 双胚苗水稻单倍体及其杂交后代基因组DNA甲基化特异位点的分析及功能探讨[J]. 中国水稻科学, 2011, 25(3): 249-255 .
WU Shao-hua,XUE Jing-jing,ZHANG Hong-yu et al. Analysis of Special DNA Methylated Sites Between Haploid of TwinSeedling and Its Hybrids in Rice[J]. , 2011, 25(3): 249-255 .
 
[1] 吴乃虎. 基因工程原理. 北京: 科学出版社, 2001: 86-88.
[2] Richards E J. DNA methylation and plant development. Trends Genet, 1997, 13: 319-323.
[3] Chan S W, Henderson I R, Jacobsen S E. Gardening the genome: DNA methylation in Arabidopsis thaliana. Nat Rev Genet, 2005, 6(5): 351-360.
[4] Bird A P, Wolffe A P. Methylation-induced repression: Belts, braces, and chromatin. Cell, 1999, 99(5): 451-454.
[5] Bestor T H. The DNA methyltransferases of mammals. Human Mol Genet, 2000, 9(16): 2395-2402.
[6] Bender J. DNA methylation and epigenetics. Annu Rev Plant Biol, 2004, 55: 41-68.
[7] Gehring M, Choi Y, Fischer R L. Imprinting and see development. Plant Cell, 2004, 16(Suppl): S203-S213.
[8] Wassenegger M. RNA-directed DNA methylation. Plant Mol Biol, 2002, 43: 203-220.
[9] Masterson J. Stomatal size in fossil plants evidence for polyploidy in majority of angiosperms. Science, 1994, 264: 421-424.
[10] Bowers J E, Chapman B A, Rong J, et al. Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events. Nature, 2003, 422: 433-438.
[11] Zhang H Y, Peng H, LI Y, et al. Patterns of DNA cytosine methylation between haploids and corresponding diploids in rice. Chin Sci Bull, 2006, 51(14): 1721-1728.
[12] Peng H, Zhang H Y, Li Y, et al. Natural homologous triploidization and DNA methylation in SARII-628, a twin-seedling rice (Oryza sativa L.). Rice Sci, 2007, 14(4): 265-271.
[13] Xiong L Z, Xu C G, Saghai Maroof M A, et al. Patterns of cytosine methylation in an elite rice hybrid and its parental lines detected by a methylation-sensitive amplification polymorphism technique. Mol Gen Genet, 1999, 261: 439-446.
[14] Mcclelland M, Nelson M, Raschke E. Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucl Acids Res, 1994, 22(17): 3640-3659.
[15] Reyna-Lopez G E, Simpson J, Ruiz-Herrera J. Differences in DNA methylation patterns are detectable during the dimorphic transition of fungi by amplification of restriction polymorphism. Mol Gen Genet, 1997, 253(6): 703-710.
[16] Okuda K, Nakamura T, Sugita M, et al. A pentatricopeptide repeat protein is a site recognition factor in chloroplast RNA editing. JBC, 2006, 281(49): 37661-37667.
[17] Zehrmann A, Verbitskiy D, van der Merwe J A, et al. A DYW domain-containing pentatricopeptide repeat protein is required for RNA editing at multiple sites in mitochondria of Arabidopsis thaliana. Plant Cell, 2009, 21: 558-567.
[18] Yi Z B, Sun Y, Niu T T, et al. Patterns of DNA cytosine methylation between hybrids and their parents in sorghum genome. Acta Agron Sin, 2005, 31(9): 1138-1143.
[19] 朱新霞, 汪保华, 郭旺珍, 等. 中棉所12配制的2个杂交棉DNA甲基化遗传与传递. 作物学报, 2009, 35(12): 2150-2158.
[20] He G M, Zhu X P, Elling A A, et al. Global epigenetic and transcriptional trends among two rice subspecies and their reciprocal hybrids. Plant Cell, 2010, 22: 17-33.
[21] 吴先军, 汪旭东, 周开达, 等. 从农艺性状和分子标记鉴定一个水稻三倍体×二倍体 F2 群体的遗传稳定性. 植物学报, 1999, 41(10): 1067-1071.
[22] 李云, 张红宇, 汪秀志, 等. 水稻同源三倍体(149-B)×二倍体F2代稳定群体的验证及分析. 遗传学报, 2004, 31(6): 604-608.
[23] Feng S, Cokus S J, Zhang X, et al. Conservation and divergence of methylation patterning in plants and animals. Proc Natl Acad Sci USA, 2010, 107(19): 8689-8694.
[24] Zemach A, McDaniel I E, Silva P, et al. Genome-wide evolutionary analysis of eukaryotic DNA methylation. Science, 2010, 328(5980): 916-919.
[1] 姜华, 赵江红, 郭龙彪, 姜亮, 薛大伟, 曾大力, 钱前, 孙国昌,. 水稻高节位分蘖的QTL定位和互作分析[J]. 中国水稻科学, 2011, 25(2): 157-162 .
[2] 徐群,袁筱萍,余汉勇,王一平,汤圣祥,魏兴华. 水稻苗期抗旱性的QTL分析[J]. 中国水稻科学, 2010, 24(5): 469-473 .
[3] 胡江,藤本宽,郭龙彪,曾大力,张光恒,董国军,王小虎,朱立煌,钱前,. 水稻抗倒力及相关抗倒伏性状的QTL分析[J]. 中国水稻科学, 2008, 22(2): 211-214 .
[4] 姜华,姜亮,赵江红,郭龙彪,孙焕明,薛大伟,曾龙军,曾大力,梁国华,钱前,. 水稻耐光氧化特性的QTL定位[J]. 中国水稻科学, 2008, 22(1): 38-44 .
[5] 彭茂民,杨国华,张菁晶,安保光,李阳生. 不同遗传背景下水稻剑叶形态性状的QTL分析[J]. 中国水稻科学, 2007, 21(3): 247-252 .
[6] 杨长登,郭龙彪,李西明,季芝娟,马良勇,钱前. 水稻抗恶苗病微效QTL的定位[J]. 中国水稻科学, 2006, 20(6): 657-659 .
[7] 杨长登,曾大力,马良勇,季芝娟,郭龙彪,李西明,钱前. 水稻籼粳交DH群体白叶枯病抗性的QTL定位[J]. 中国水稻科学, 2006, 20(1): 102-104 .
[8] 张光恒, 张国平, 钱前, 徐律平, 曾大力, 滕胜, 包劲松. 不同环境条件下稻谷粒形数量性状的QTL分析[J]. 中国水稻科学, 2004, 18(1): 16-22 .
[9] 寒川一成,滕 胜,钱 前,曾大力,曾 娟,钱忠海,朱立煌. 水稻籼粳交DH群体中影响白背飞虱抗虫性QTL的检测[J]. 中国水稻科学, 2003, 17(ZK): 77-83 .
[10] 于彦春, 滕胜, 曾大力, 董国军, 钱前, 黄大年, 朱立煌,. 水稻抗白叶枯病微效QTL的定位分析[J]. 中国水稻科学, 2003, 17(4): 315-318 .
[11] 董国军, 藤本宽, 滕胜, 胡兴明, 曾大力, 郭龙彪, 钱前,. 水稻剑叶角度的QTL分析[J]. 中国水稻科学, 2003, 17(3): 219-222 .
[12] 曹立勇 , 朱 军 , 颜启传 , 何立斌 , 魏兴华 , 程式华 . 水稻籼粳交DH群体幼苗中胚轴长度的QTLs定位和上位性分析[J]. 中国水稻科学, 2002, 16(3): 221-224 .
[13] 曾大力 ,钱 前 ,阮刘青 ,滕 胜 ,国广泰史 ,藤本 宽 ,朱立煌,. 稻米垩白三维切面的遗传分析[J]. 中国水稻科学, 2002, 16(1): 11-14 .
[14] 程式华, 毛传澡, 占小登, 斯华敏, 孙宗修. 籼粳交DH群体和RIL群体的构建及籼粳分化[J]. 中国水稻科学, 2001, 15(4): 257-260 .
[15] 严长杰,李 欣,程祝宽,于恒秀,顾铭洪,朱立煌. 利用分子标记定位水稻芽期耐冷性基因[J]. 中国水稻科学, 1999, 13(3): 134-138 .
[16] 杨长登,吴连斌,赵成章. 单倍体籼稻无性系微芽的离体调控[J]. 中国水稻科学, 1998, 12(4): 219-222 .
[17] 杨长登 ,唐克轩 ,吴连斌 ,李 瑶 ,赵成章 ,刘光杰 ,沈大棱 . 农杆菌介导将雪莲凝集素(GNA)基因转入籼稻单倍体微芽的初步研究[J]. 中国水稻科学, 1998, 12(3): 129-133 .
版权所有 © 《中国水稻科学》编辑部 浙ICP备05004719号-5
地 址:浙江省杭州市体育场路359号   邮 编:310006   电 话:0571-63370278   E-mail:cjrs@263.net
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn