中国水稻科学

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一个水稻叶片白化转绿叶突变体的遗传分析和精细定位

郭士伟1; 王永飞2; 马三梅2; 李霞1;高东迎1   

  1. 1江苏省农业科学院 粮食作物研究所/江苏省优质水稻工程技术研究中心, 江苏 南京210014; 2暨南大学 生物工程学系, 广东 广州 510632
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-10 发布日期:2011-01-10

Genetic Analysis and Fine Mapping of a GreenRevertible Albino Leaf Mutant in Rice

GUO Shi-wei1; WANG Yong-fei2; MA San-mei2; LI Xia1; GAO Dong-ying1   

  1. 1 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R & D Center, Nanjing 210014, China; 2Department of Bioengineering, Jinan University, Guangzhou 510632, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-10 Published:2011-01-10

摘要: 在水稻品种宜香B中发现了一个白化转绿叶突变体,经过多代自交获得了稳定的白化转绿叶色突变体。该突变体在4叶期前叶色为黄绿色,之后逐渐变绿,从苗龄4周到12周,突变体/野生型叶绿素含量比值从34.5%逐渐升高到99.4%。遗传分析表明该突变受1对隐性核基因控制,暂命名为gra。利用微卫星标记将gra初步定位于第10染色体标记RM596和RM5620之间,进一步利用极端个体定位法把gra精细定位于标记RM25522和RM25535之间。gra基因距RM25522和RM25535标记的遗传距离均为0.05 cM, 其物理距离约为136 kb。

关键词: 水稻, 白化转绿叶突变体

Abstract: A greenrevertible albino leaf mutant was found in an indica rice variety, Yixiang B, in the field. A stably inherited mutant with greenrevertible albino leaf was obtained after five successive generations of selfcrossing, and temporarily named as gra. The leaf color of gra was albino before 4th leaf stage, and then gradually turned green. As the content of chlorophyll in leaves increased from 4weekold to 12weekold seedlings, the ratio of mutant/wild chlorophyll content in rice leaves rose from 34.5% to 99.4%. Genetic analysis of F2 populations showed that the mutation was controlled by a single recessive nuclear gene. The gene was finely mapped between the SSR markers RM25522 and RM25535, with genetic distances of 0.05 cM to each marker, respectively, and a physical distance of 136 kb.

Key words: rice, greenrevertible albino leaf mutant, gene, fine mapping