中国水稻科学

• 实验技术 • 上一篇    下一篇

一种快速同步检测水稻黑条矮缩病毒和南方水稻黑条矮缩病毒的方法

季英华1;高瑞珍1, 2 ;张野1;程兆榜1;周彤1;范永坚1;周益军1, *   

  1. 1江苏省农业科学院 植物保护研究所, 江苏 南京 210014; 2扬州大学 园艺与植物保护学院, 江苏 扬州 225009;*通讯联系人, E-mail: yjzhou@jaas.ac.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-10 发布日期:2011-01-10

A Simplified Method for Quick Detection of Rice BlackStreaked Dwarf Virus and Southern Rice BlackStreaked Dwarf Virus

JI Ying-hua1; GAO Rui-zhen1, 2;ZHANG Ye1; CHENG Zhao-bang1;ZHOU Tong1;FAN Yong-jian1;ZHOU Yi-jun1, *   

  1. 1Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; *Corresponding author, E-mail: yjzhou@jaas.ac.cn
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-10 Published:2011-01-10

摘要: 水稻黑条矮缩病毒和南方水稻黑条矮缩病毒是近年来在水稻上危害日益严重的两种病毒,由于两者同属于呼肠孤病毒科斐济病毒属,在症状、粒体形状、传播介体及寄主等方面非常相似,由此也给诊断及鉴定造成了困难。针对这一问题,通过设计简并引物,并优化体系,建立了一种快速、简便、灵敏、特异的方法,为这两种病毒的检测及鉴别提供了方便。

关键词: 水稻黑条矮缩病毒, 南方水稻黑条矮缩病毒, 检测, 方法

Abstract: Rice blackstreaked dwarf virus and southern rice blackstreaked dwarf virus are two important viruses in rice, which cause great losses in local rice production in recent years. The two viruses belong to Fijivirus. They share many similar characters, such as symptoms, virus particles, vectors, host range etc., causing difficulty in discrimination of the two viruses. Three degenerate primers were designed and a simple, rapid, efficient method was described.

Key words: rice blackstreaked dwarf virus, Southern rice blackstreaked dwarf virus, detection, methodology