Dynamic Changes of Resistance to Fipronil and Neonicotinoid Insecticides in Brown Planthopper, Nilaparvata lugens(Homoptera: Delphacidae)
ZHOU Wei-jun3, LUO Cai-hong3
1Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, China; 2Institute of Quality and Standard for Agro products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;3Institute of Shenzhen Noposion Agrochemical CO. Ltd.， Shenzhen 518102, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: jlshen@njau.edu.cn
The resistances to the phenyl pyrazole (such as fipronil), neonicotinoid and insect growth regulator insecticides (buprofezin) in brown planthopper, Nilaparvata lugens (Stl) were monitored by ricestem dipping method during 2006 to 2008. Results showed that N. lugens sharply developed resistance to fipronil following its immigration route in 2008. The levels of resistance to Fipronil in 10 immigration populations from 6 provinces in the Yangtze River Delta areas and the backmigration populations in Shaoguan and Shenzhen of Guangdong were moderate (resistance ratio 15.0-fold to 32.5-fold) and high (66.9-fold to 73.7-fold), respectively, higher than those of all 6 field populations from 5 provinces in 2006(2.6-fold to 5.8-fold) and 11 populations from 9 provinces in 2007 (3.2-fold to 8.4-fold). The above results suggest that N. lugens would develop a higher level of resistance to fipronil in most ricegrowing areas in China during 2009 to 2010. All populations collected in 2006-2008 showed high or extremely high level of resistance to imidacloprid, of which the resistance levels of 13 populations from 8 provinces in 2008 were 210.1-fold to 381.7-fold, though the resistance level dropped slightly in the following 3 years comparing with the extremely high level in 2005 (277fold to 811fold). For other neonicotinoid insecticides such as hiamethoxam, nitenpyram and dinotefuran, the resistance ratios were in the range of 20fold to 158fold, 07fold to 48fold, and 06fold to 28fold, respectively. All the populations were susceptible or lowlevel resistant to buprofezin (30fold to 119fold). In recent years, widespread and intensive use of fipronil for controlling two classes of migratory rice pest insects (brown planthopper/whitebacked planthopper and rice leaf roller) might be a major reason for fipronil resistance outbreaking in N. lugens. Therefore, to prevent insecticide resistance in N. lugens from outbreaking again, insecticide resistance management strategy including alternating and rotating insecticides without crossresistance need to be established.
ZHOU Wei-jun,LUO Cai-hong,$author.xingMing_EN et al. Dynamic Changes of Resistance to Fipronil and Neonicotinoid Insecticides in Brown Planthopper, Nilaparvata lugens(Homoptera: Delphacidae)[J]. , 2010, 24(1): 73-80 .
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2010, Vol. 24 
