中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

两份转Fer基因富铁水稻特异种质应用价值的初步评价

叶红霞1;郭泽建2;舒庆尧1;徐晓晖3;包劲松1; 沈圣泉1，*   

  1. 1 浙江大学 农业与生物技术学院， 浙江 杭州 310029； 2 中国农业大学 植物病理系， 北京 100094； 3 中国计量学院 生命科学学院， 浙江 杭州 310018; *通讯联系人, E-mail: shenshq@zju.edu.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-05-10 发布日期:2007-05-10

Evaluation and Application of Two High-Iron Transgenic Rice Lines with a Pea Ferritin Gene

YE Hong-xia , GUO Ze-jian , SHU Qing-yao , XU Xiao-hui , BAO Jin-song , SHEN Sheng-quan   

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-10 Published:2007-05-10

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被引次数

51

摘要： 在获得了105份转豌豆铁蛋白基因（Fer）水稻转化体的基础上，通过连续7代自交纯化，并结合GUS标记辅助选择，得到了82份独立转基因纯系。经稻米矿质元素检测，发掘到2份富铁转基因种质（Fer34和Fer65），其精米全铁含量为野生型亲本秀水11的4.82倍和3.46倍。进一步鉴定表明，所筛选到的2份富铁转基因种质的外源基因（Fer）遗传简单，增加精米粉铁含量明显，对植株重要农艺性状、其他矿质营养、精米外观和食用品质等均无明显的负作用，是较为难得的富铁转基因优良新种质。此外，还就该2份富铁种质的实用价值及其应用前景进行了讨论。

关键词: 转基因水稻, 豌豆铁蛋白基因, 矿质营养, 稻米品质

Abstract: A total of 105 transgenic rice lines independently transformed with a pea ferritin gene(Fer) were previously obtained.After seven generations of self-crossing and GUS marker assisted selection,82 homozygous transgenic rice lines were used to evaluate their potential applications as high-iron rice germplasm.Two high-iron transgenic rice lines Fer34 and Fer65,with the iron content in the milled rice 4.82 and 3.46 times of the wild parent Xiushui 11 were identified.Further analysis indicated that the exogenous Fer gene was highly expressed,and inherited as a single locus.The transgene had no negative effect on the agronomic traits of rice plants,other mineral nutritional components,appearance and eating quality of the milled rice,indicating that they were elite high-iron breeding lines.Furthermore,the practical application and further studies facilitating utilization of the two elite breeding lines were discussed.

Key words: transgenic rice, pea ferritin gene, mineral nutrition, rice quality