中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻抗性淀粉突变体抗性淀粉结构的比较研究

焦桂爱1;唐绍清1;罗炬1; Melissa FITZGERALD2; Leslie T. ROFEROS2 ;胡培松1,*   

  1. 中国水稻研究所, 浙江 杭州 310006； 2 国际水稻研究所, 菲律宾 马尼拉 DAPO 7777信箱； *通讯联系人， E-mail: riceh@sohu.com
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-11-10 发布日期:2006-11-10

Comparative Study on Resistant Starch Structure of Resistant Starch Enriched Rice Mutants

JIAO Gui-ai , TANG Shao-qing , LUO Ju , Melissa FITZGERALD , Leslie T. ROFEROS , HU Pei-song   

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10

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被引次数

58

摘要： 利用凝胶渗透色谱法和毛细管电泳法对2个富含抗性淀粉的水稻突变体RS111、AE及其野生型亲本R7954、IR36的抗性淀粉组成结构进行了比较研究，分析了2个突变体与野生型亲本抗性淀粉中直链淀粉和支链淀粉的差异。 RS111的抗性淀粉主要来源于直链淀粉，支链淀粉所占比例很小，直链淀粉与支链淀粉的比率相对于野生型亲本来说大大提高，从抗性淀粉中支链淀粉的链长分布来看，RS111与R7954比较一致，只是RS111中聚合度为10的短链比例明显小于亲本R7954；而另一突变体AE的抗性淀粉来源中，支链淀粉所占比例很大，直链淀粉与支链淀粉的比率相对于野生型亲本来说变化不大，支链淀粉中聚合度为10左右的短链比例大大高于亲本IR36，而聚合度为16、21左右的中长链比例远远低于亲本。

关键词: 抗性淀粉结构, 突变体, 直链淀粉, 支链淀粉, 支链淀粉链长分布

Abstract: Two resistant starch enriched rice mutants RS111,AE and the wild type rice R7954 and IR36 were compared in the resistant starch structure by using gel permeation chromatography and capillary electrophoresis.The main component of the resistant starch in RS111 was amylose,the ratio of amylose to amylopectin was much higher compared to the wild type R7954,and the resistant starch amylopectin chain length distribution in RS111 was similar to the wild type,only the proportion of short chains of DP(degree of polymerization)=10 was decreased;Howerver,for the resistant starch in AE,the proportion of amylose and amylopectin were almost equal,amylose and amylopectin were both increased,and amylose to amylopectin ratio was not distinctively different from the wild type IR36,but the amylopectin chain length distribution was much different from the wild type,the proportion of short chains of DP=10 was much higher,but the proportion of chains of DP= 16 and 21 was much lower compared with the wild type IR36.

Key words: resistant starch structure, mutant, amylose, amylopectin, amylopectin chain length distribution