中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

灌浆结实期高温对水稻籽粒蔗糖及降解酶活性的影响

李天1;刘奇华1;大杉立2;山岸徹2;佐佐木治人2   

  1. 1 四川农业大学 农学院， 四川 雅安 625014； Email: lghhlt@hotmail.com； 2 东京大学 大学院 农学生命科学研究科， 日本东京文京区113-8567
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-11-10 发布日期:2006-11-10

Effect of High Temperature on Sucrose Content and Sucrose-Cleaving Enzymes Activity in Rice during Grain Filling Stage

LI Tian , LIU Qi-hua , Ryu OHSUGI , Tohru YAMAGISHI , Haruto SASAKI   

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10

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被引次数

65

摘要： 选用水稻品种越光和笹锦为材料，在开花后设置自然温度和高温两种处理，研究了不同温度处理下籽粒蔗糖、果糖和葡萄糖含量的动态变化以及蔗糖合酶、液泡型转化酶和细胞壁结合型转化酶活性的差异。高温处理下，蔗糖合酶活性值大于转化酶活性值，且与淀粉积累速率呈显著正相关，表明蔗糖合酶在蔗糖的分解和淀粉的合成过程中起着重要作用；高温下两品种籽粒的蔗糖含量明显增加，但分解的葡萄糖和果糖含量并未相应增加，表明高温有利于籽粒中蔗糖的积累，而不利于籽粒中蔗糖的分解。高温下蔗糖合酶、液泡型转化酶和细胞壁结合型转化酶活性明显下降，表明蔗糖分解速率的下降与蔗糖合酶和转化酶活性下降有关。

关键词: 高温, 蔗糖, 蔗糖合酶, 液泡型转化酶, 细胞壁结合型转化酶, 水稻, 籽粒

Abstract: The dynamic changes of sucrose,fructose,glucose contents and differences in sucrose synthase(SS),vacuolar invertase(VCI),and cell wall bound invertase(CWI) activities in rice grain after flowering were studied under natural and high temperatures by using two japonica varieties Koshihikari and Sasanishiki as materials.In rice grains,sucrose synthase activity was higher than invertase activity,and positively significantly correlated with starch accumulation rate,indicating that sucrose synthase played an important role in sucrose degradation and starch synthesis.Sucrose content in rice grains(increased) significantly,whereas fructose and glucose contents did not increase correspondingly under high temperature,suggesting that high temperature treatment enhanced sucrose accumulation,but diminished sucrose degradation in rice grains.Sucrose synthase,vacuolar invertase,and cell wall bound invertase activities in rice grains at high temperature decreased definitely compared with controls,showing that the deceleration of sucrose-degradation rate was related to the decrease of(sucrose) synthase and invertase activities.

Key words: high temperature, sucrose, sucrose synthase, vacuolar invertase, cell wall bound invertase, rice, grain