中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

抗除草剂转基因水稻稳定系TR4的获得及其遗传研究

吴明国;黄大年;林建荣;汪晓玲;华志华;章善庆;薛 锐   

  1. 中国水稻研究所，浙江 杭州 310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-07-10 发布日期:1999-07-10

Establishment and Genetic Analysis of a Stable Herbicide Resistant Transgenic Rice Line TR4

Wu Mingguo; Huang Danian; Lin Jianrong; Wang Xiaoling; Hua Zhihua; Zhang Shangqing; Xue Rui   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-07-10 Published:1999-07-10

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摘要： 用Bar基因通过基因枪法轰击京引119未成熟胚，得到抗除草剂Basta的转基因植株，经加代和选择得到抗除草剂的稳定系TR4，其基本农艺性状与京引119相仿。用TR4与4个粳稻品种杂交进行遗传分析表明，TR4的抗除草剂特性由一对显性基因控制，符合孟德尔遗传规律。该外源基因可通过有性杂交转育，在杂交稻和常规直播稻的选育上有较大的利用价值。

关键词: 水稻, 抗除草剂, 转基因植株

Abstract: Immature embryos of rice variety Jingyin ll9 were treated with bar gene via particle bombardment. Herbicide resistant transgenic rice plantlets were obtained. The stable herbicide resistant transgenic rice line TR4,which possesses agronomic characters similar to Jingyin 1l9,was consecutively selected from the self-pollinated progenies of above transformed plant. Genetic analysis of the crosses of TR4 with four japonica rice varieties demonstrated that the herbicide resistance of TR4 was controlled by a pair of dominant genes and accorded with Mendelian segregation. Successful transbreeding of bar gene by sexual hybridization is valuable in selection of hybrid rice and direct seeded rice.

Key words: rice, herbicide resistance, transgenic plant, genetic analysis