中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

利用分子标记定位水稻芽期耐冷性基因

严长杰1;李 欣1;程祝宽1;于恒秀1;顾铭洪1;朱立煌2   

  1. 1扬州大学 农学院 农学系，江苏 扬州 225009; 2 中国科学院遗传研究所，北京 100101
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-07-10 发布日期:1999-07-10

Identification of QTL for Cold Tolerance at Early Seedling Stage in Rice(Oryza sativa) via RFLP Markers

Yan Changjie; Li Xin; Cheng Zhukuan; Yu Hengxiu; Gu Minghong; Zhu Lihuang   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-07-10 Published:1999-07-10

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摘要： 利用籼稻品种南京11和粳稻品种巴利拉的F1花药进行组织培养，获得67个加倍单倍体植株(Doubled haploid, DH)。 以该群体构建了一张包含131个RFLP标记的水稻分子图谱。 以芽期的死苗率为指标， 评定亲本及各 DH系在低温(4～5℃)条件下的耐冷性。结果表明：死苗率在DH群体中呈双峰连续分布， 表明芽期耐冷性是由主基因控制的数量性状。将死苗率作为数量性状进行QTL的区间作图分析， 发现在第7染色体上G379b-RG4区间存在有与耐冷性有关的基因(Cts7) 。

关键词: 加倍单倍体, 芽期, 耐冷性, 基因定位, 水稻

Abstract: By using a doubled haploid population(DH) containing 67 DH lines derived from anther culture of F1 cross between Balilla (japonica) and Nanjing 11 (indica), an RFLP map was constructed, which consisted of 131 RFLP markers covering all 12 linkage groups at an average distance 14. 1 cm. When the cold tolerance was assessed by using died seedling percent in DH lines and their parents at 4 - 5 C as the indicator, a continuous distribution with two peaks was observed in the DHpopulation. The result indicated that the cold tolerance at early seedling stage in rice fitted for quantitative trait model, andwas controlled by a major gene. On the basis of QTL interval analysis with Mapmaker/QTL, 39% of the phenotypic variance associated with died seedling percent was explained by the QTL(Cts 7), which was at the interval G379b-RG4 on chromosome7. The mapping of cold tolerance gene Cts 7 can facilitate the rice cold tolerance breeding via marker assisted selection.

Key words: doubled haploid, cold tolerance, seedling stage, gene location, rice