中国水稻科学

• 综述与专论 • 上一篇    下一篇

转座子标签法克隆水稻基因前景

朱正歌; 孙宗修   

  1. 中国水稻研究所 农业部水稻生物学重点实验室,浙江 杭州 310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-01-10 发布日期:2001-01-10

Prospect of Cloning Rice Gene with Transposable Element Tagging

ZHU Zheng-ge; SUN Zong-xiu

  

  1. (The Key Laboratory for Rice Biology; Ministry of Agriculture; P.R.China; China National Rice Research Institute; Hangzhou 310006; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-01-10 Published:2001-01-10

摘要: 转座子标签法克隆基因是近年来发展起来的一种非常有效的分子生物技术。介绍了转座子标签技术克隆基因的基本原理及研究进展,并对转座子标签技术在水稻基因克隆上的应用进行了讨论分析。

关键词: 功能基因组学, 转座子, 克隆, 基因, 水稻

Abstract: Transposon tagging is a powerful molecular bio-technique for isolating genes which encode unknown gene products, allowing genes, identified only by their mutant phenotype, to be cloned in recent years. The fundamentals and studied developments on transposon tagging are reviewed as well as the prospect of transposon tagging in rice genes is discussed and evaluated.

Key words: functional genomics, transposon, clone, gene, rice