中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

应用候选基因定位水稻抗稻瘟病QTL

樊叶杨1; 吴建利1; 庄杰云1; Hei LEUNG 2; 郑康乐 1,*   

  1. 1中国水稻研究所 国家水稻改良中心， 浙江 杭州 310006; 2 国际水稻研究所 昆虫植病部， 菲律宾 马尼拉3127信箱； * 通讯联系人
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-10-10 发布日期:2001-10-10

Mapping of QTL for Rice Blast Resistance by Using Candidate Genes

FAN Ye-yang 1; WU Jian-li 1; ZHUANG Jie-yun 1; Hei LEUNG 2; ZHENG Kang-le 1   

  1. (1National Center for Rice Improvement; China National Rice Research Institute; Hangzhou 310006; China; 2Entomology and Plant Pathology Division; International Rice Research Institute, PO Box 3127, 1271 Makati City, Philippines)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-10-10 Published:2001-10-10
  • Contact: ZHENG Kang-le

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被引次数

50

摘要： 应用经克隆了的已知功能或有潜在功能的DNA序列,即候选基因，作为分子标记，在中156/谷梅2号F8重组自交系群体中进行水稻抗稻瘟病QTL的分析。大部分候选基因在水稻染色体上成簇分布，并且位于已知抗病基因簇区域。应用复合区间法检测到1个调控病斑大小和1个调控病斑数量的QTL，前者位于第1染色体CG36a~RM212区间，贡献率为4.17%，抗性等位基因来自父本谷梅2号；后者定位于第2染色体CG18a~RM263区间，贡献率为6.25%，抗性等位基因来自母本中156。同时检测到2对控制病叶面积和1对控制病斑大小的基因互作。这些QTL和互作基因涉及抗性基因同源序列、离子通道调控子以及编码致病相关蛋白和几丁质酶的基因，表明候选基因的应用有助于揭示QTL的功能。玉米锈病抗性基因Rp1与稻瘟病抗性有关，提示了利用水稻这个模式作物来克隆较大基因组中有利基因的可能性。

关键词: 水稻, 稻瘟病, 候选基因, 数量性状位点

Abstract: Candidate genes, deriving from cloned and characterized DNA sequences associated with known or potential functions, were employed to detect QTL for blast resistance in an F8 recombined inbred population constructed from the cross Zhong 156/Gumei 2. Most candidate genes were clusterly distributed in regions of known resistance gene clusters. One QTL for lesion size (LS) and another QTL for lesion number (LN) were detected. QTL qLS-1 located in the interval of CG36a-RM212 on chromosome 1 explained 4.17% of the phenotypic variance, and the resistance allele was from Gumei 2. QTL qLN-2 located in the interval of CG18a-RM263 on chromosome 2 explained 6.25% of the phenotypic variance, and the resistance allele was from Zhong 156. Three pairs of significant digenic interactions were also found, among which two controlled diseased leaf area and one controlled LS. Candidate genes associated with resistance to rice blast were related to several types, including resistance gene homologues, ion channel regulator, and genes encoding pathogenicity related protein and chitinase. The results indicated that the application of candidate genes might facilitate the discovery of the functions of resistance QTL. In addition, Rp1 conditioning leaf rust resistance in maize showed association with rice blast resistance, indicating the possibility of ultilizing rice genome for cloning genes in larger genome.

Key words: rice, blast, candidate gene, quantitative trait locus