中国水稻科学

• 研究报告 • 上一篇    下一篇

籼粳交DH群体和RIL群体的构建及籼粳分化

程式华1,2; 毛传澡1; 占小登1; 斯华敏2; 孙宗修2   

  1. 1中国水稻研究所 国家水稻改良中心,浙江 杭州 310006; 2 中国水稻研究所 农业部水稻生物学重点实验室,浙江 杭州 310006;E-mail: shcheng@mail.hz.zj.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-10-10 发布日期:2001-10-10

Construction of Double Haploid (DH) and Recombinant Inbred Line (RIL) Population of Indica-Japonica Hybrid and Their Differential in Indica and Japonica Property

CHENG Shi-hua; MAO Chuan-zao; ZHAN Xiao-deng; SI Hua-min; SUN Zong-xiu   

  1. (China National Rice Research Institute; Hangzhou 310006; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-10-10 Published:2001-10-10

摘要: 对籼粳杂交组合“02428(粳)/特青(籼)”F1进行一步成苗法花药培养,对该组合的分离世代采用连续自交随机选择,分别获得由132个株系组成的加倍单倍体(DH)群体和由157个株系组成的重组自交系(RIL)群体。通过形态指数法和分子标记法对两个群体的籼粳分化进行了研究,结果表明,无论是DH群体还是RIL群体,各株系的籼粳形态指数和分子标记指数分布均呈正态分布,但RIL群体的偏态性强于DH群体,且明显偏父。

关键词: 加倍单倍体, 重组自交系

Abstract: A double haploid (DH) population with 132 plant lines and a recombination inbred lines (RIL) with 152 plant lines were constructed through one step anther culture and continuous self pollination of the hybrid between 02428 (japonica) and Teqing (indica), respectively. The differentiation of indica and japonica in the two populations was detected by the morphological index and RFLP method. All the morphological traits of DH and RIL populations were in continuous segregation and showed normal distribution. It was indicated that the segregation and recombination of genes of indica and japonica in DH population were basically random and the overall frequency of alleles did not deviate from the ratio of 1∶1. But RIL population was significantly tended to the indica parent.

Key words: double haploid, recombination inbred line, differentiation of indica and japonica