中国水稻科学

中国水稻科学

• 实验技术 • 上一篇    下一篇

一种叶片直接用作PCR扩增的新方法及其应用

汪秀峰; 杨剑波; 向太和; 李 莉; 倪大虎   

  1. 安徽省农业科学院， 水稻遗传育种农业部重点开放实验室， 安徽 合肥 230031
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-01-10 发布日期:2002-01-10

A New Method for PCR Reaction from Alkali-Treated Rice Leaf Tissues

WANG Xiu-feng; YANG Jian-bo; XIANG Tai-he; LI Li; NI Da-hu   

  1. (Key Laboratory of Rice Genetic and Breeding of Agricultural Ministry of China; Anhui Academy of Agricultural Sciences; Hefei 230031; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-01-10 Published:2002-01-10

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摘要： 以水稻为模式植物研究了利用碱处理叶片直接用作PCR扩增的模板，并应用于水稻品种种性和纯度鉴定以及分子标记辅助田间育种材料的选择中。该方法具有快速、简便、结果可信度高，重复性好，对待测植株的损伤小等特点，它无需高速冷冻离心机及相应的提取DNA的试剂，故试验成本低,检测所需的时间短。尤其是群体较大时，此种方法更是显得经济有效，利用此种方法在1 d内可检测300份样品。

关键词: 水稻, 基因组DNA, 分子标记, 聚合酶链式反应, 鉴定, 研究方法

Abstract: A new method for PCR reaction from a small quantity of rice leaf tissue with alkali-treated was presented, which can be applied either to identify genotype of rice variety and its purity, or to assist selection for molecular breeding. This method is rapid, simple and reliable, and only needs a small part of rice leaf tissue that does not damage the whole plant. It does not need high speed refrigerated centrifuge and the reagents for DNA extraction. Especially, it is more economic and effective for a large-scale PCR tests. Normally 300 samples can be processed within a day by this method.

Key words: rice, genomic DNA, molecular marker, polymerse chain reaction, identification, methodology