中国水稻科学

中国水稻科学

• 实验技术 • 上一篇    下一篇

水稻AFLP指纹银染法显带研究

朱正歌 1,3; 贾继增 2; 孙宗修 1   

  1. 1中国水稻研究所， 农业部水稻生物学重点实验室， 浙江 杭州 310006； 2中国农业科学院 作物品种资源研究所， 农业部作物品种资源与生物技术重点实验室， 北京 100081； 3河北师范大学 生命科学学院， 河北 石家庄 050016
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-01-10 发布日期:2002-01-10

Improvement of AFLP Protocol for Silver-Staining in Rice

ZHU Zheng-ge 1; 3; JIA Ji-zeng 2; SUN Zong-xiu 1   

  1. (1 The Key Laboratory for Rice Biology; Ministry of Agriculture; China National Rice Research Institute; Hangzhou 310006; China; 2The Key Laboratory for Crop Germplasm Resources and Biotechnology, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-01-10 Published:2002-01-10

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摘要： 以转基因水稻为材料，对AFLP指纹银染技术及实验操作中的关键技巧做了比较详尽的叙述，取得了比较理想的实验结果。

关键词: 随机片段长度多态性, 银染, 实验技术

Abstract: Using transgenic rice as materials, the technique of AFLP silver-staining protocol and some skills were specified, and excellent results were obtained.

Key words: amplified fragment length polymorphism, silver-staining, experimental technique