抗条纹叶枯病基因STV11的功能性分子标记建立及粳型抗源筛选

doi:10.16819/j.1001-7216.2016.6058

摘要/Abstract

摘要：

条纹叶枯病是水稻(Oryza sativa L.)上最严重的病害之一。STV11是从籼稻Kasalath中鉴定的条纹叶枯病抗性基因,根据Kasalath型条纹叶枯病基因STV11^KAS6个碱基缺失的功能性多态性序列差异,设计基于PCR的功能性分子标记qstv11。利用此标记对299份试验材料[5份抗病品种、5份感病品种、41份辽宁省育种材料、17份日本粳型材料、142份秋光/七山占重组自交系(群体A)、85份笹锦/Habataki//笹锦回交重组自交系(群体B)、群体亲本4份]进行基因型分析并进行抗病性鉴定。结果表明,qstv11能够较好区分抗感基因型,而且在测试材料中与条纹叶枯抗性整体呈现共分离。41份辽宁材料中鉴定出含有STV11-R(抗)材料1份;日本粳型材料该位点均为STV11-S (感);群体A和B中分别筛选出粳稻背景STV11-R型材料24份和6份。

关键词: 水稻, 条纹叶枯病, STV11, 功能性标记, 粳型材料

Abstract:

The Rice stripe virus (RSV) causes one of the most serious viral diseases of rice (Oryza sativa L.). STV11 is a rice stripe virus resistant gene derived from india rice Kasalath. We developed a simple PCR-based functional marker named qstv11 which targets the functional six bp delection polymorphism at STV11^KAS. The genotype on STV11 and rice stripe virus resistance of 299 varieties was analyzed, including five resistant varieties, five susceptible varieties, 41 varieties from Liaoning Province, 17 Japanese japonica varieties, 142 recombinant inbred lines (Akihikari/Qishanzhan,population A), 85 backcross recombinant inbred lines (Sasanishiki/Habataki//Sasanishiki population,B) and 4 parents. The newly developed functional marker was highly efficient in discriminating resistant genotypes from susceptible ones and showed perfect co-segregation with the trait of rice stripe virus resistance in the test varieties. One variety “Kangyan 100” was identified with STV11-R from Liaoning varieties. All the varieties from Japanese japonica varieties were susceptible to rice stripe virus. Twenty four and six varieties were identified with STV-R from population A and population B, respectively. Several varieties with STV11-R type with japonica background were detected.

Key words: rice, rice stripe virus, STV11, functional marker, japonica type

中图分类号:

毛艇, 李旭, 张战, 付立东, 李振宇. 抗条纹叶枯病基因STV11的功能性分子标记建立及粳型抗源筛选[J]. 中国水稻科学, 2016, 30(6): 661-667.

Ting MAO, Xu LI, Zhan ZHANG, Li-dong FU, Zhen-yu LI. Development of Functional Marker for Rice stripe virus Resistant Gene STV11 and Resistant Germplasm Selection in japonica Rice[J]. Chinese Journal OF Rice Science, 2016, 30(6): 661-667.

图/表 4

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编号 Number	品种(系)名称 Variety (or line) name	亚种属性 Subspecies	基因型 Genotype	抗病鉴定Phenotype
编号 Number	品种(系)名称 Variety (or line) name	亚种属性 Subspecies	基因型 Genotype	2014	2015
1	特青 Teqing	I	STV11-R	HR	HR
2	扬稻8号 Yangdao 8	I	STV11-R	HR	HR
3	2134	I	STV11-R	HR	HR
4	BJ 1	I	STV11-R	HR	HR
5	Modan	I	STV11-R	HR	HR
6	越光 Koshihikari	J	STV11-S	S	MS
7	沈农265 Shennong 265	J	STV11-S	MS	S
8	辽粳294 Liaojing 294	J	STV11-S	S	S
9	辽粳287 Liaojing 287	J	STV11-S	MS	S
10	牡丹江2号 Mudanjiang 2	J	STV11-S	S	S
11	七山占 Qishanzhan	I	STV11-R	HR	HR
12	秋光 Akihikari	J	STV11-S	MS	S
13	Habataki	I	STV11-R	HR	HR
14	屉锦 Sasanishiki	J	STV11-S	HS	HS
15	盐粳68 Yanjing 68	J	STV11-S	R	R
16	盐粳933 Yanjing 933	J	STV11-S	S	S
17	盐粳 98 Yanjing 98	J	STV11-S	MS	S
18	盐粳 188 Yanjing 188	J	STV11-S	HS	HS
19	盐粳 48 Yanjing 48	J	STV11-S	S	MS
20	盐粳 218 Yanjing 218	J	STV11-S	R	R
21	盐粳228 Yanjing 228	J	STV11-S	MS	S
22	盐粳 927 Yanjing 927	J	STV11-S	S	S
23	盐粳 939 Yanjing 939	J	STV11-S	HS	HS
24	盐粳 166 Yanjing 166	J	STV11-S	HS	HS
25	桥科 951 Qiaoke 951	J	STV11-S	HS	HS
26	盐丰47 Yanfeng 47	J	STV11-S	R	R
27	龙盘5号 Longpan 5	J	STV11-S	HS	S
28	辽星1号 Liaoxing 1	J	STV11-S	S	MS
29	辽星21 Liaoxing 21	J	STV11-S	HS	HS
30	辽粳9号 Liaojing 9	J	STV11-S	S	MS
31	辽粳371 Liaojing 371	J	STV11-S	HS	HS
32	辽粳294 Liaojing 294	J	STV11-S	HS	HS
33	盐粳50 Yanjing 50	J	STV11-S	S	MS
34	盐粳16 Yanjing 16	J	STV11-S	HS	HS
35	沈农9816 Shennong 9816	J	STV11-S	HS	S
36	辽盐2号 Liaoyan 2	J	STV11-S	R	R
37	盐粳456 Yanjing 456	J	STV11-S	S	MS
38	沈农9816 Shennong 9816	J	STV11-S	S	S
39	沈农9903 Shennong 9903	J	STV11-S	HS	S
40	港源8号 Gangyuan 8	J	STV11-S	R	R
41	铁粳1号 Tiejing 1	J	STV11-S	S	HS
42	铁粳7号 Tiejing 7	J	STV11-S	MS	S
43	辽粳5号 Liaojing 5	J	STV11-S	S	S
44	辽粳326 Liaojing 326	J	STV11-S	MS	MS
45	LDC119	J	STV11-S	S	S
46	抗盐100 Kangyan 100	J	STV11-R	HR	HR
47	辽盐241 Liaoyan 241	J	STV11-S	S	S
48	铁粳12 Tiejing 12	J	STV11-S	S	S
49	铁粳9号 Tiejing 9	J	STV11-S	S	MS
50	铁粳11 Tiejing 11	J	STV11-S	S	S
51	铁粳7号 Tiejing 7	J	STV11-S	S	S
52	富禾90 Fuhe 90	J	STV11-S	MS	S
53	美锋9号 Meifeng 9	J	STV11-S	S	S
54	美锋1号 Meifeng 1	J	STV11-S	S	MS
55	铁粳6号 Tiejing 6	J	STV11-S	S	S
56	A1	J	STV11-R	R	R
编号 Number	品种(系)名称 Variety (or line) name	亚种属性 Subspecy	基因型 Genotype	抗病鉴定Phenotype
编号 Number	品种(系)名称 Variety (or line) name	亚种属性 Subspecy	基因型 Genotype	2014	2015
57	A2	J	STV11-R	HR	R
58	A3	J	STV11-R	R	HR
59	A4	J	STV11-R	R	HR
60	A5	J	STV11-R	R	R
61	A6	J'	STV11-R	HR	R
62	A7	J'	STV11-R	R	R
63	A8	J'	STV11-R	HR	HR
64	A9	J'	STV11-R	R	HR
65	A10	J'	STV11-R	HR	R
66	A11	J'	STV11-R	R	R
67	A12	J'	STV11-R	HR	R
68	A13	J'	STV11-R	R	R
69	A14	J'	STV11-R	R	HR
70	A15	J'	STV11-R	R	R
71	A16	I'	STV11-R	R	HR
72	A17	I'	STV11-R	HR	R
73	A18	I'	STV11-R	R	R
74	A19	J	STV11-R	HR	R
75	A20	J'	STV11-R	R	R
76	A21	J'	STV11-R	HR	HR
77	A22	J'	STV11-R	R	R
78	A23	J'	STV11-R	HR	R
79	A24	J'	STV11-R	R	R
80	A25	J'	STV11-R	R	R
81	A26	J'	STV11-R	HR	HR
82	A27	J'	STV11-R	R	R
83	B1	J	STV11-R	R	HR
84	B2	J	STV11-R	R	HR
85	B3	J	STV11-R	R	HR
86	B4	J'	STV11-R	R	R
87	B5	J'	STV11-R	HR	HR
88	B6	J'	STV11-R	R	R
89	生拔 Shengba	J	STV11-S	S	MS
90	接冠 Jieguan	J	STV11-S	MS	S
91	取手1号Toride 1	J	STV11-S	S	S
92	誉锦 Yujin	J	STV11-S	S	S
93	青系96 Aokei 96	J	STV11-S	S	MS
94	福锦 Fukunishiki	J	STV11-S	S	S
95	一目惚 Hitomebore	J	STV11-S	MS	S
96	福响 Fukuhibiki	J	STV11-S	HS	HS
97	岁华 Suihua	J	STV11-S	S	HS
98	丰锦 Toyonishiki	J	STV11-S	S	S
99	新秋誉 Xinqiuyu	J	STV11-S	MS	S
100	秋田小町 Akitakomachi	J	STV11-S	S	S
101	初星 Hatsuboshi	J	STV11-S	S	S
102	出羽粲 Chuyucan	J	STV11-S	S	MS
103	农林3号 Norin 3	J	STV11-S	MS	S
104	秀峰 Syuuhou	J	STV11-S	S	S
105	山形80 Yamagata 80	J	STV11-S	MS	S

编号 Number	品种(系)名称 Variety (or line) name	亚种属性 Subspecies	基因型 Genotype	抗病鉴定Phenotype
编号 Number	品种(系)名称 Variety (or line) name	亚种属性 Subspecies	基因型 Genotype	2014	2015
1	特青 Teqing	I	STV11-R	HR	HR
2	扬稻8号 Yangdao 8	I	STV11-R	HR	HR
3	2134	I	STV11-R	HR	HR
4	BJ 1	I	STV11-R	HR	HR
5	Modan	I	STV11-R	HR	HR
6	越光 Koshihikari	J	STV11-S	S	MS
7	沈农265 Shennong 265	J	STV11-S	MS	S
8	辽粳294 Liaojing 294	J	STV11-S	S	S
9	辽粳287 Liaojing 287	J	STV11-S	MS	S
10	牡丹江2号 Mudanjiang 2	J	STV11-S	S	S
11	七山占 Qishanzhan	I	STV11-R	HR	HR
12	秋光 Akihikari	J	STV11-S	MS	S
13	Habataki	I	STV11-R	HR	HR
14	屉锦 Sasanishiki	J	STV11-S	HS	HS
15	盐粳68 Yanjing 68	J	STV11-S	R	R
16	盐粳933 Yanjing 933	J	STV11-S	S	S
17	盐粳 98 Yanjing 98	J	STV11-S	MS	S
18	盐粳 188 Yanjing 188	J	STV11-S	HS	HS
19	盐粳 48 Yanjing 48	J	STV11-S	S	MS
20	盐粳 218 Yanjing 218	J	STV11-S	R	R
21	盐粳228 Yanjing 228	J	STV11-S	MS	S
22	盐粳 927 Yanjing 927	J	STV11-S	S	S
23	盐粳 939 Yanjing 939	J	STV11-S	HS	HS
24	盐粳 166 Yanjing 166	J	STV11-S	HS	HS
25	桥科 951 Qiaoke 951	J	STV11-S	HS	HS
26	盐丰47 Yanfeng 47	J	STV11-S	R	R
27	龙盘5号 Longpan 5	J	STV11-S	HS	S
28	辽星1号 Liaoxing 1	J	STV11-S	S	MS
29	辽星21 Liaoxing 21	J	STV11-S	HS	HS
30	辽粳9号 Liaojing 9	J	STV11-S	S	MS
31	辽粳371 Liaojing 371	J	STV11-S	HS	HS
32	辽粳294 Liaojing 294	J	STV11-S	HS	HS
33	盐粳50 Yanjing 50	J	STV11-S	S	MS
34	盐粳16 Yanjing 16	J	STV11-S	HS	HS
35	沈农9816 Shennong 9816	J	STV11-S	HS	S
36	辽盐2号 Liaoyan 2	J	STV11-S	R	R
37	盐粳456 Yanjing 456	J	STV11-S	S	MS
38	沈农9816 Shennong 9816	J	STV11-S	S	S
39	沈农9903 Shennong 9903	J	STV11-S	HS	S
40	港源8号 Gangyuan 8	J	STV11-S	R	R
41	铁粳1号 Tiejing 1	J	STV11-S	S	HS
42	铁粳7号 Tiejing 7	J	STV11-S	MS	S
43	辽粳5号 Liaojing 5	J	STV11-S	S	S
44	辽粳326 Liaojing 326	J	STV11-S	MS	MS
45	LDC119	J	STV11-S	S	S
46	抗盐100 Kangyan 100	J	STV11-R	HR	HR
47	辽盐241 Liaoyan 241	J	STV11-S	S	S
48	铁粳12 Tiejing 12	J	STV11-S	S	S
49	铁粳9号 Tiejing 9	J	STV11-S	S	MS
50	铁粳11 Tiejing 11	J	STV11-S	S	S
51	铁粳7号 Tiejing 7	J	STV11-S	S	S
52	富禾90 Fuhe 90	J	STV11-S	MS	S
53	美锋9号 Meifeng 9	J	STV11-S	S	S
54	美锋1号 Meifeng 1	J	STV11-S	S	MS
55	铁粳6号 Tiejing 6	J	STV11-S	S	S
56	A1	J	STV11-R	R	R
编号 Number	品种(系)名称 Variety (or line) name	亚种属性 Subspecy	基因型 Genotype	抗病鉴定Phenotype
编号 Number	品种(系)名称 Variety (or line) name	亚种属性 Subspecy	基因型 Genotype	2014	2015
57	A2	J	STV11-R	HR	R
58	A3	J	STV11-R	R	HR
59	A4	J	STV11-R	R	HR
60	A5	J	STV11-R	R	R
61	A6	J'	STV11-R	HR	R
62	A7	J'	STV11-R	R	R
63	A8	J'	STV11-R	HR	HR
64	A9	J'	STV11-R	R	HR
65	A10	J'	STV11-R	HR	R
66	A11	J'	STV11-R	R	R
67	A12	J'	STV11-R	HR	R
68	A13	J'	STV11-R	R	R
69	A14	J'	STV11-R	R	HR
70	A15	J'	STV11-R	R	R
71	A16	I'	STV11-R	R	HR
72	A17	I'	STV11-R	HR	R
73	A18	I'	STV11-R	R	R
74	A19	J	STV11-R	HR	R
75	A20	J'	STV11-R	R	R
76	A21	J'	STV11-R	HR	HR
77	A22	J'	STV11-R	R	R
78	A23	J'	STV11-R	HR	R
79	A24	J'	STV11-R	R	R
80	A25	J'	STV11-R	R	R
81	A26	J'	STV11-R	HR	HR
82	A27	J'	STV11-R	R	R
83	B1	J	STV11-R	R	HR
84	B2	J	STV11-R	R	HR
85	B3	J	STV11-R	R	HR
86	B4	J'	STV11-R	R	R
87	B5	J'	STV11-R	HR	HR
88	B6	J'	STV11-R	R	R
89	生拔 Shengba	J	STV11-S	S	MS
90	接冠 Jieguan	J	STV11-S	MS	S
91	取手1号Toride 1	J	STV11-S	S	S
92	誉锦 Yujin	J	STV11-S	S	S
93	青系96 Aokei 96	J	STV11-S	S	MS
94	福锦 Fukunishiki	J	STV11-S	S	S
95	一目惚 Hitomebore	J	STV11-S	MS	S
96	福响 Fukuhibiki	J	STV11-S	HS	HS
97	岁华 Suihua	J	STV11-S	S	HS
98	丰锦 Toyonishiki	J	STV11-S	S	S
99	新秋誉 Xinqiuyu	J	STV11-S	MS	S
100	秋田小町 Akitakomachi	J	STV11-S	S	S
101	初星 Hatsuboshi	J	STV11-S	S	S
102	出羽粲 Chuyucan	J	STV11-S	S	MS
103	农林3号 Norin 3	J	STV11-S	MS	S
104	秀峰 Syuuhou	J	STV11-S	S	S
105	山形80 Yamagata 80	J	STV11-S	MS	S