中国水稻科学

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (4): 476-480.DOI: 10.3969/j.issn.10017216.2012.04.013

• 研究报告 • 上一篇    下一篇

OsDUF500基因沉默提高水稻对白叶枯病的抗性

李娟,常闪闪,刘凤权,邵敏*   

  1. 南京农业大学 教育部病虫监测与治理重点开放实验室， 江苏 南京 210095;
  • 收稿日期:2011-05-12 修回日期:2012-03-10 出版日期:2012-07-10 发布日期:2012-07-10
  • 通讯作者: 邵敏*
  • 基金资助:

    国家863计划资助项目（2008AA10Z108, 2007AA10Z188）； 国家转基因生物新品种培育重大专项（2009ZX08001005B）。

Silencing of OsDUF500 Gene in Rice Enhances Resistance to Xanthomonas oryzae pv. oryzae

 LI  Juan, CHANG  Shanshan, LIU  Fengquan, SHAO Min*   

  1. Key Laboratory of Monitoring and Management of Plant Diseases and Insects， Ministry of Education，Nanjing Agricultural University， Nanjing 210095， China；
  • Received:2011-05-12 Revised:2012-03-10 Online:2012-07-10 Published:2012-07-10
  • Contact: SHAO Min*

PDF

摘要： 以编码HarpinXoo 的hrf1转基因水稻NJH12的cDNA为模板，克隆到了DUF500的保守功能区。序列分析表明，OsDUF500是DUF500家族成员，功能未知。构建OsDUF500的沉默载体，用土壤根癌农杆菌介导的方法转化粳稻 R109，用Hpt抗生素做抗性筛选，获得9个再生株系。经分子鉴定，8个为沉默株系（ABD2~9）。与野生型相比， ABD株系株高变矮，叶片变短，出现白色空秕谷。白叶枯病抗性鉴定结果表明，OsDUF500沉默株系对水稻白叶枯病的抗性存在差异，病斑长度比野生型明显缩短，病斑面积均在30%以下，株系ABD3和ABD5病斑面积分别为12.3%和15.7%，显示出较好的抗性。推测在稻瘟病抗性中上调表达基因OsDUF500对水稻白叶枯病抗性起负调控作用。

关键词: OsDUF500, 转基因水稻, 白叶枯病, 抗性

Abstract: Conserved functional region of DUF500 was cloned by PCR from cDNA of expression HarpinXoo (hrf1) transgenic rice NJH12. OsDUF500 encodes an unknown function member of DUF500 family. Calli from mature embryos of rice cultivar R109 were transformed by Agrobacterium tumefaciens strain EHA105/ABD::pVec8. There were eight OsDUF500 transgenic lines from nine hygromycin resistant lines by PCR analysis. Compared with the wild type, the downexpressed lines ABD are characterized by reduced plant height, shortened leaf and white blighted grain. Disease assays of transgenic rice plants infected by Xanthomonas oryzae pv. oryzae (PXO99) indicated that different transgenic lines had different resistance to rice leaf blight. Lesion length of the downexpression lines reduced significantly and lesion area all bellowed 30%. Lesion areas of the ABD3 and ABD5 were 12.3% and 15.7%, respectively. Thus, we predicted OsDUF500gene, which was upregulated in rice blast, maybe was a negative regulator on bacterial blight resistance. 

Key words: OsDUF500 gene, transgenic rice, Xanthomonas oryzae pv. oryzae, resistance

中图分类号: