中国水稻科学 ›› 2023, Vol. 37 ›› Issue (2): 142-152.DOI: 10.16819/j.1001-7216.2023.220603
魏倩倩1,2, 徐青山2, 潘林2, 孔亚丽2, 朱练峰2, 曹小闯2, 田文昊2, 刘佳3, 金千瑜2, 项兴佳1, 张均华2,*(), 朱春权2,*()
收稿日期:
2022-06-08
修回日期:
2022-08-31
出版日期:
2023-03-10
发布日期:
2023-03-10
通讯作者:
张均华,朱春权
基金资助:
WEI Qianqian1,2, XU Qingshan2, PAN Lin2, KONG Yali2, ZHU Lianfeng2, CAO Xiaochuang2, TIAN Wenhao2, LIU Jia3, JIN Qianyu2, XIANG Xingjia1, ZHANG Junhua2,*(), ZHU Chunquan2,*()
Received:
2022-06-08
Revised:
2022-08-31
Online:
2023-03-10
Published:
2023-03-10
Contact:
ZHANG Junhua, ZHU Chunquan
摘要:
【目的】阐明钙离子与硫化氢相互作用缓解水稻铝毒害的分子和生理机制。【方法】以Kasalath为试验材料,选取0 μmol/L和30 μmol/L AlCl3,0.1 mmol/L 和 0.5 mmol/L CaCl2,0.2 μmol/L NaHS和100 μmol/L硫化氢清除剂亚牛磺酸(HP)作为处理浓度,将种子置于30℃培养箱中黑暗培养24 h后取水稻根系,通过测定水稻根系伸长量、总铝含量、细胞汁液中铝含量、质外体中铝含量、细胞壁中铝含量、果胶含量、果胶甲酯酶活性以及OsSTAR2、OsNRAT1和OsFRDL4相对表达量,探究钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制。【结果】铝胁迫下,相较于0.1 mmol/L CaCl2处理,0.5 mmol/L CaCl2处理显著提高了水稻根系伸长量、硫化氢含量、总钙含量和细胞质中钙含量,显著降低了水稻根系的总铝含量,细胞液、质外体和细胞壁中的铝含量。铝胁迫下,硫氢化钠预处理后,水稻根系的伸长量在两种钙浓度下均显著增加,水稻的根尖铝含量、根系总铝含量、细胞液中铝含量、质外体中铝含量和细胞壁中铝含量在两种钙浓度下均显著降低,OsSTAR2和OsFRDL4相对表达量在两种钙浓度下均显著提高,OsNRAT1相对表达量在两种钙浓度下均显著降低。铝胁迫下,添加HP则呈现相反的结果。【结论】铝胁迫下,钙离子通过增加水稻根系硫化氢的生成,降低水稻根系对铝的吸收和积累,最终缓解铝对水稻根系伸长的抑制作用。
魏倩倩, 徐青山, 潘林, 孔亚丽, 朱练峰, 曹小闯, 田文昊, 刘佳, 金千瑜, 项兴佳, 张均华, 朱春权. 钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制[J]. 中国水稻科学, 2023, 37(2): 142-152.
WEI Qianqian, XU Qingshan, PAN Lin, KONG Yali, ZHU Lianfeng, CAO Xiaochuang, TIAN Wenhao, LIU Jia, JIN Qianyu, XIANG Xingjia, ZHANG Junhua, ZHU Chunquan. Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice[J]. Chinese Journal OF Rice Science, 2023, 37(2): 142-152.
引物 Primer | 序列Sequence (5′-3′) |
---|---|
OsSTAR2-R | CCTCAGCTTCTTCATCGTCACC |
OsSTAR2-F | ACCTCTTCATGGTCACCGTCG |
OsFRDL4-R | TCATTTGCGAAGAAACTTCCACG |
OsFRDL4-F | CGTCATCAGCACCATCCACAG |
OsNRAT1-F | GAGGCCGTCTGCAGGAGAGG |
OsNRAT1-R | GGAAGTATCTGCAAGCAGCTCTGATGC |
OsHistone-R | AACCGCAAAATCCAAAGAACG |
OsHistone-F | GGTCAACTTGTTGATTCCCCTCT |
表1 本研究所选的引物及其序列
Table 1. Primers selected for this study and the sequences.
引物 Primer | 序列Sequence (5′-3′) |
---|---|
OsSTAR2-R | CCTCAGCTTCTTCATCGTCACC |
OsSTAR2-F | ACCTCTTCATGGTCACCGTCG |
OsFRDL4-R | TCATTTGCGAAGAAACTTCCACG |
OsFRDL4-F | CGTCATCAGCACCATCCACAG |
OsNRAT1-F | GAGGCCGTCTGCAGGAGAGG |
OsNRAT1-R | GGAAGTATCTGCAAGCAGCTCTGATGC |
OsHistone-R | AACCGCAAAATCCAAAGAACG |
OsHistone-F | GGTCAACTTGTTGATTCCCCTCT |
图1 不同铝浓度对水稻根系伸长(A)和根尖铝含量(B)的影响 不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。
Fig. 1. Effects of different Al3+ concentrations on root elongation (A) and Al content in root apices (B). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3).
图2 不同钙浓度对水稻根系伸长(A)和根尖铝含量(B)的影响 不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。?Al表示不加Al;+Al表示Al3+浓度为30μmol/L。
Fig. 2. Effects of different Ca2+ concentrations on root elongation (A) and Al content in root apices (B). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). -Al, No Al3+ addition; +Al, 30μmol/L Al3+.
图3 不同钙处理对水稻根系总铝含量(A)、细胞液铝含量(B),质外体铝含量(C),细胞壁铝含量(D),果胶含量(E)和果胶甲酯酶活性(F)的影响 不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。
Fig. 3. Effects of different Ca2+ concentrations on total Al concentration (A), Al concentration in cell sap (B), apoplastic Al concentration (C), Al content in cell wall(D), pectin content (E), pectin methylesterase activity (F). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.5Ca, 0.5 mmol/L CaCl2; 0.1Ca+Al, 0.1 mmol/L CaCl2+30 μmol/L AlCl3; 0.5Ca+Al, 0.5 mmol/L CaCl2+30 μmol/L AlCl3.
图4 不同钙处理对水稻根系总钙含量(A)和细胞质中钙含量(B和C)影响 不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。
Fig. 4. Effects of different Ca2+ concentrations on total Ca concentration (A), Ca concentration in cell sap (B and C). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca: 0.1 mmol/L CaCl2; 0.5Ca: 0.5 mmol/L CaCl2; 0.1Ca+Al: 0.1 mmol/L CaCl2+30 μmol/L AlCl3; 0.5Ca+Al: 0.5 mmol/L CaCl2+30 μmol/L AlCl3.
图5 不同钙处理对水稻根系内源性硫化氢含量的影响 不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。
Fig. 5. Effects of different Ca2+ concentrations on the content of endogenous H2S. Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca: 0.1 mmol/L CaCl2; 0.5Ca: 0.5 mmol/L CaCl2; 0.1Ca+Al: 0.1 mmol/L CaCl2+30 μmol/L AlCl3; 0.5Ca+Al: 0.5 mmol/L CaCl2+30 μmol/L AlCl3.
图6 钙离子与硫化氢相互作用对水稻根系伸长(A)、相对根系伸长(B)和根尖铝含量(C)的影响。 不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。
Fig. 6. Effects of interaction between Ca2+ and H2S on root elongation (A), relative root elongation (B) and Al content in root apices (C). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.1Ca+NaHS, 0.1 mmol/L CaCl2+0.2 μmol/L NaHS; 0.1Ca+HP, 0.1 mmol/L CaCl2+100 μmol/L HP; 0.5Ca, 0.5 mmol/L CaCl2; 0.5Ca+NaHS, 0.5 mmol/L CaCl2+0.2 μmol/L NaHS; 0.5Ca+HP, 0.5 mmol/L CaCl2+100 μmol/L HP.
图7 钙离子与硫化氢相互作用对水稻根系中总铝含量(A),细胞液铝含量(B),质外体铝含量(C),细胞壁铝含量(D),果胶含量(E)和果胶甲酯酶活性(F)的影响 不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。
Fig. 7. Effects of interaction between Ca2+ and H2S on total Al concentration (A), Al concentration in cell sap (B), apoplastic Al concentration (C), Al content in cell wall(D), pectin content (E), pectin methylesterase activity (F). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.1Ca+NaHS, 0.1 mmol/L CaCl2+0.2 μmol/L NaHS; 0.1Ca+HP, 0.1 mmol/L CaCl2+100 μmol/L HP; 0.5Ca, 0.5 mmol/L CaCl2; 0.5Ca+NaHS, 0.5 mmol/L CaCl2+0.2 μmol/L NaHS; 0.5Ca+HP, 0.5 mmol/L CaCl2+100 μmol/L HP.
图8 钙离子与硫化氢相互作用对水稻根系OsSTAR2 (A)、OsFRDL4 (B)和OsNRAT1 (C)相对表达量的影响 不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。
Fig. 8. Effects of interaction between Ca2+ and H2S on relative expression of OsSTAR2 (A), OsFRDL4 (B) and OsNRAT1 (C). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.1Ca+NaHS, 0.1 mmol/L CaCl2+0.2 μmol/L NaHS; 0.1Ca+HP, 0.1 mmol/L CaCl2+100 μmol/L HP; 0.5Ca, 0.5 mmol/L CaCl2; 0.5Ca+NaHS, 0.5 mmol/L CaCl2+0.2 μmol/L NaHS; 0.5Ca+HP, 0.5 mmol/L CaCl2+100 μmol/L HP.
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