钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制

doi:10.16819/j.1001-7216.2023.220603

中国水稻科学 ›› 2023, Vol. 37 ›› Issue (2): 142-152.DOI: 10.16819/j.1001-7216.2023.220603

钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制

魏倩倩¹^,², 徐青山², 潘林², 孔亚丽², 朱练峰², 曹小闯², 田文昊², 刘佳³, 金千瑜², 项兴佳¹, 张均华²^,^*(), 朱春权²^,^*()

¹安徽大学资源与环境工程学院, 合肥 230601
²中国水稻研究所水稻生物学国家重点实验室, 杭州 310006
³江西省农业科学院土壤肥料与资源环境研究所, 南昌 330200

收稿日期:2022-06-08 修回日期:2022-08-31 出版日期:2023-03-10 发布日期:2023-03-10
通讯作者: 张均华,email: zhangjunhua@caas.cn;朱春权,email: zhuchunquan@caas.cn
基金资助:
国家自然科学基金青年项目(31901452);国家自然科学基金青年项目(42207415);国家自然科学基金青年项目(32201702);国家自然科学基金面上项目(31872857);国家自然科学基金面上项目(31771733);国家重点研发计划课题(2022YFD1500403);国家重点研发计划课题(2022YFD2301404-2);水稻生物学国家重点实验室开放课题资助项目(20190402)

Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice

WEI Qianqian¹^,², XU Qingshan², PAN Lin², KONG Yali², ZHU Lianfeng², CAO Xiaochuang², TIAN Wenhao², LIU Jia³, JIN Qianyu², XIANG Xingjia¹, ZHANG Junhua²^,^*(), ZHU Chunquan²^,^*()

¹School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China
²State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
³Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

Received:2022-06-08 Revised:2022-08-31 Online:2023-03-10 Published:2023-03-10
Contact: ZHANG Junhua, email: zhangjunhua@caas.cn;ZHU Chunquan, email: zhuchunquan@caas.cn

摘要/Abstract

摘要：

【目的】阐明钙离子与硫化氢相互作用缓解水稻铝毒害的分子和生理机制。【方法】以Kasalath为试验材料，选取0 μmol/L和30 μmol/L AlCl₃，0.1 mmol/L 和 0.5 mmol/L CaCl₂，0.2 μmol/L NaHS和100 μmol/L硫化氢清除剂亚牛磺酸(HP)作为处理浓度，将种子置于30℃培养箱中黑暗培养24 h后取水稻根系，通过测定水稻根系伸长量、总铝含量、细胞汁液中铝含量、质外体中铝含量、细胞壁中铝含量、果胶含量、果胶甲酯酶活性以及OsSTAR2、OsNRAT1和OsFRDL4相对表达量，探究钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制。【结果】铝胁迫下，相较于0.1 mmol/L CaCl₂处理，0.5 mmol/L CaCl₂处理显著提高了水稻根系伸长量、硫化氢含量、总钙含量和细胞质中钙含量，显著降低了水稻根系的总铝含量，细胞液、质外体和细胞壁中的铝含量。铝胁迫下，硫氢化钠预处理后，水稻根系的伸长量在两种钙浓度下均显著增加，水稻的根尖铝含量、根系总铝含量、细胞液中铝含量、质外体中铝含量和细胞壁中铝含量在两种钙浓度下均显著降低，OsSTAR2和OsFRDL4相对表达量在两种钙浓度下均显著提高，OsNRAT1相对表达量在两种钙浓度下均显著降低。铝胁迫下，添加HP则呈现相反的结果。【结论】铝胁迫下，钙离子通过增加水稻根系硫化氢的生成，降低水稻根系对铝的吸收和积累，最终缓解铝对水稻根系伸长的抑制作用。

关键词: 水稻, 铝胁迫, 钙离子, 硫化氢

Abstract:

【Objective】 The objective of this study is to elucidate the molecular and physiological mechanisms of the interaction between calcium ion (Ca²⁺) and hydrogen sulfide (H₂S) alleviating aluminum (Al) toxicity in rice.【Method】 The rice cultivar kasalath was selected as test material, and treated with 0 μmol/L and 30 μmol/L AlCl₃, 0.1 mmol/L and 0.5 mmol/L CaCl₂, 0.2 μmol/L NaHS and 100 μmol/L H₂S scavenger hypotaurine (HP). The seeds were incubated at 30℃ in the dark for 24 h, then the root elongation, total Al content, Al content in cell sap, Al content in apoplast, Al content in cell wall, pectin content, pectin methylesterase activity and the relative expression of OsSTAR2, OsNRAT1 and OsFRDL4 were measured to explore the molecular and physiological mechanism of the interaction between Ca²⁺ and H₂S alleviating Al toxicity in rice. 【Result】 Under Al stress, compared with 0.1 mmol/L CaCl₂ treatment, 0.5 mmol/L CaCl₂ treatment significantly increased root elongation, H₂S content, total calcium (Ca) content and cytoplasm Ca content, and significantly decreased total Al content in root, cell sap Al content, apoplastic Al content and cell wall Al content in rice. The pretreatment of NaHS significantly increased the elongation of rice roots at the two calcium concentrations, accompanied with the significant increase of root tip Al content, root total Al content, cell sap Al content, apoplastic Al content and cell wall Al content. The relative expression levels of OsSTAR2 and OsFRDL4 were significantly increased and the relative expression of OsNRAT1 was significantly decreased at the two Ca²⁺ concentrations after pretreated with NaHS. However, the application of HP produced an opposite result. 【Conclusion】 Under Al stress, Ca²⁺ reduced the accumulation of Al in rice root cell wall and the absorption of Al by rice roots through increasing the production of H₂S in rice root, and finally alleviated the inhibition of Al on rice root elongation.

Key words: rice, aluminum stress, calcium ion, hydrogen sulfide

魏倩倩, 徐青山, 潘林, 孔亚丽, 朱练峰, 曹小闯, 田文昊, 刘佳, 金千瑜, 项兴佳, 张均华, 朱春权. 钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制[J]. 中国水稻科学, 2023, 37(2): 142-152.

WEI Qianqian, XU Qingshan, PAN Lin, KONG Yali, ZHU Lianfeng, CAO Xiaochuang, TIAN Wenhao, LIU Jia, JIN Qianyu, XIANG Xingjia, ZHANG Junhua, ZHU Chunquan. Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice[J]. Chinese Journal OF Rice Science, 2023, 37(2): 142-152.

图/表 9

表1 本研究所选的引物及其序列

Table 1. Primers selected for this study and the sequences.

引物 Primer	序列Sequence (5′-3′)
OsSTAR2-R	CCTCAGCTTCTTCATCGTCACC
OsSTAR2-F	ACCTCTTCATGGTCACCGTCG
OsFRDL4-R	TCATTTGCGAAGAAACTTCCACG
OsFRDL4-F	CGTCATCAGCACCATCCACAG
OsNRAT1-F	GAGGCCGTCTGCAGGAGAGG
OsNRAT1-R	GGAAGTATCTGCAAGCAGCTCTGATGC
OsHistone-R	AACCGCAAAATCCAAAGAACG
OsHistone-F	GGTCAACTTGTTGATTCCCCTCT

图1 不同铝浓度对水稻根系伸长（A）和根尖铝含量（B）的影响不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。

Fig. 1. Effects of different Al3+ concentrations on root elongation (A) and Al content in root apices (B). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3).

图2 不同钙浓度对水稻根系伸长（A）和根尖铝含量（B）的影响不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。?Al表示不加Al；+Al表示Al3+浓度为30μmol/L。

Fig. 2. Effects of different Ca2+ concentrations on root elongation (A) and Al content in root apices (B). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). -Al, No Al3+ addition; +Al, 30μmol/L Al3+.

图3 不同钙处理对水稻根系总铝含量（A）、细胞液铝含量（B），质外体铝含量（C），细胞壁铝含量（D），果胶含量（E）和果胶甲酯酶活性（F）的影响不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。

Fig. 3. Effects of different Ca2+ concentrations on total Al concentration (A), Al concentration in cell sap (B), apoplastic Al concentration (C), Al content in cell wall(D), pectin content (E), pectin methylesterase activity (F). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.5Ca, 0.5 mmol/L CaCl2; 0.1Ca+Al, 0.1 mmol/L CaCl2+30 μmol/L AlCl3; 0.5Ca+Al, 0.5 mmol/L CaCl2+30 μmol/L AlCl3.

图4 不同钙处理对水稻根系总钙含量（A）和细胞质中钙含量（B和C）影响不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。

Fig. 4. Effects of different Ca2+ concentrations on total Ca concentration (A), Ca concentration in cell sap (B and C). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca: 0.1 mmol/L CaCl2; 0.5Ca: 0.5 mmol/L CaCl2; 0.1Ca+Al: 0.1 mmol/L CaCl2+30 μmol/L AlCl3; 0.5Ca+Al: 0.5 mmol/L CaCl2+30 μmol/L AlCl3.

图5 不同钙处理对水稻根系内源性硫化氢含量的影响不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。

Fig. 5. Effects of different Ca2+ concentrations on the content of endogenous H2S. Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca: 0.1 mmol/L CaCl2; 0.5Ca: 0.5 mmol/L CaCl2; 0.1Ca+Al: 0.1 mmol/L CaCl2+30 μmol/L AlCl3; 0.5Ca+Al: 0.5 mmol/L CaCl2+30 μmol/L AlCl3.

图6 钙离子与硫化氢相互作用对水稻根系伸长（A）、相对根系伸长（B）和根尖铝含量（C）的影响。不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。

Fig. 6. Effects of interaction between Ca2+ and H2S on root elongation (A), relative root elongation (B) and Al content in root apices (C). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.1Ca+NaHS, 0.1 mmol/L CaCl2+0.2 μmol/L NaHS; 0.1Ca+HP, 0.1 mmol/L CaCl2+100 μmol/L HP; 0.5Ca, 0.5 mmol/L CaCl2; 0.5Ca+NaHS, 0.5 mmol/L CaCl2+0.2 μmol/L NaHS; 0.5Ca+HP, 0.5 mmol/L CaCl2+100 μmol/L HP.

图7 钙离子与硫化氢相互作用对水稻根系中总铝含量（A），细胞液铝含量（B），质外体铝含量（C），细胞壁铝含量（D），果胶含量（E）和果胶甲酯酶活性（F）的影响不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。

Fig. 7. Effects of interaction between Ca2+ and H2S on total Al concentration (A), Al concentration in cell sap (B), apoplastic Al concentration (C), Al content in cell wall(D), pectin content (E), pectin methylesterase activity (F). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.1Ca+NaHS, 0.1 mmol/L CaCl2+0.2 μmol/L NaHS; 0.1Ca+HP, 0.1 mmol/L CaCl2+100 μmol/L HP; 0.5Ca, 0.5 mmol/L CaCl2; 0.5Ca+NaHS, 0.5 mmol/L CaCl2+0.2 μmol/L NaHS; 0.5Ca+HP, 0.5 mmol/L CaCl2+100 μmol/L HP.

图8 钙离子与硫化氢相互作用对水稻根系OsSTAR2 (A)、OsFRDL4 (B)和OsNRAT1 (C)相对表达量的影响不同字母代表处理间的差异达0.05显著水平。数据为平均值±标准差(n=3)。

Fig. 8. Effects of interaction between Ca2+ and H2S on relative expression of OsSTAR2 (A), OsFRDL4 (B) and OsNRAT1 (C). Different letters in the figure indicate that there is a significant difference in the results under the analysis of variance (P<0.05), and the value is the mean ± standard deviation (n=3). 0.1Ca, 0.1 mmol/L CaCl2; 0.1Ca+NaHS, 0.1 mmol/L CaCl2+0.2 μmol/L NaHS; 0.1Ca+HP, 0.1 mmol/L CaCl2+100 μmol/L HP; 0.5Ca, 0.5 mmol/L CaCl2; 0.5Ca+NaHS, 0.5 mmol/L CaCl2+0.2 μmol/L NaHS; 0.5Ca+HP, 0.5 mmol/L CaCl2+100 μmol/L HP.

参考文献 41

[1]	郑爱珍, 李春喜. 酸性红壤铝毒对植物的影响及其改良[J]. 湖北农业科学, 2004(6): 38-40.
	Zheng A Z, Li C X. Influences and improvement of Al toxin on plant in acid red soil[J]. Huibei Agricultural Sciences, 2004(6): 38-40. (in Chinese)
[2]	Poschenrieder C, Gunsé B, Corrales I, Barceló J J. A glance into aluminum toxicity and resistance in plants[J]. The Science of the Total Environment, 2008, 400(1-3): 356-368. DOI URL
[3]	Zhen Y, Qi J L, Wang S S, Su J, Xu G H, Zhang M S, Miao L, Peng X X, Tian D C, Yang Y H. Comparative proteome analysis of differentially expressed proteins induced by Al toxicity in soybean[J]. Physiologia Plantarum, 2007, 131: 542-554. DOI URL
[4]	Zhu C Q, Zhang J H, Sun L M, Zhu L F, Abliz B, Hu W J, Zhong C, Bai Z G, Sajid H, Cao X C, Jin Q Y. Hydrogen sulfide alleviates aluminum toxicity via decreasing apoplast and symplast Al contents in rice[J]. Frontiers in Plant Science, 2018, 9: 294 DOI PMID
[5]	沈仁芳. 铝在土壤-植物中的行为及植物的适应机制[M]. 北京: 科学出版社, 2008.
	Shen R F. The Behavior of Aluminum in Soil Plant and the Adaptive Mechanism of Plants[M]. Beijing: Science Press, 2008. (in Chinese)
[6]	Ma J F, Chen Z C, Shen R F. Molecular mechanisms of Al tolerance in gramineous plants[J]. Plant and Soil, 2014, 381: 1-12. DOI URL
[7]	Huang W J, Oo T L, He H Y, Wang A Q, Zhan J, Wei S Q, He L F. Aluminum induces rapidly mitochondria- dependent programmed cell death in Al sensitive peanut root tips[J]. Botanical Studies, 2014, 55: 67. DOI URL
[8]	Hepler P K. Calcium: A central regulator of plant growth and development[J]. The Plant Cell, 2005: 2142-2155.
[9]	Tang R H, Han S C, Zheng H L, Cook C W, Choi C S, Woerner T E, Jackson R B, Pei Z M. Coupling diurnal cytosolic Ca²⁺ oscillations to the CAS-IP3 pathway in Arabidopsis[J]. Science, 2007, 315: 1423-1426. DOI URL
[10]	Zhu J K. Abiotic stress signaling and responses in plants[J]. Cell, 2016, 167(2): 313-324. DOI URL
[11]	Wan U L, Najeeb U, Jilani G, Naeem M S, Zhou W J. Calcium invigorates the cadmium-stressed Brassica napus L. Plants by strengthening their photosynthetic system[J]. Environmental Science and Pollution Research International, 2011, 18: 1478-1486. DOI URL
[12]	Bashir K, Rasheed S, Kobayashi T, Seki M, Nishizawa N K. Regulating subcellular metal homeostasis: The key to crop improvement[J]. Front Plant Science, 2016, 7: 1192.
[13]	Lu H P, Li Z, Wu J T, Shen Y, Li Y W, Zou B, Tang Y T, Zhuang P. Influences of Calcium silicate on chemical forms and subcellular distribution of cadmium in Amaranthus hypochondriacus L[J]. Scientific Reports, 2017, 7: 40583. DOI
[14]	王爱勤, 何龙飞, 沈振国, 刘友良, 李杨瑞. 铝胁迫下钙对小麦幼苗营养元素吸收和转运的影响[J]. 西南农业学报, 2002, 15 (2): 42-47.
	Wang A Q, He L F, Shen Z G, Liu Y L, Li Y R. Effects of calcium on the absorption and distribution of nutrient elements of wheat seedlings under aluminum stress[J]. Southwest China Journal of Agricultural Sciences, 2002, 15(2): 42-47.
[15]	Chen J, Duan R X, Hu W J, Zhang N N, Lin X Y, Zhang J H, Zheng H L. Unravelling calcium-alleviated aluminium toxicity in Arabidopsis thaliana: Insights into regulatory mechanisms using proteomics[J]. Journal of Proteomic, 2019, 199: 15-30. DOI URL
[16]	Fang H H, Jing T, Liu Z Q, Zhang L Q, Jin Z P, Pei Y X. Hydrogen sulfide interacts with calcium signaling to enhance the chromium tolerance in Setaria italica[J]. Cell Calcium, 2014, 56(6): 472-481. DOI URL
[17]	Bont L D, Mu X J, Wei B, Han Y. Abiotic stress-triggered oxidative challenges: Where does H₂S act?[J]. Journal of Genetics and Genomics, 2022, 49(8): 748-755. DOI URL
[18]	Peng R Y, Bian Z Y, Zhou L, Cheng W, Hai N, Yang C Q, Yang T, Wang X Y, Wang C Y. Hydrogen sulfide enhances nitric oxide-induced tolerance of hypoxia in maize (Zea mays L.)[J]. Plant Cell Reports, 2016, 35(11): 2325-2340. DOI URL
[19]	Wang H H, Ji F, Zhang Y Y, Hou J J, Liu W W, Huang J J, Liang W H. Interactions between hydrogen sulphide and nitric oxide regulate two soybean citrate transporters during the alleviation of aluminium toxicity[J]. Plant, Cell and Environment, 2019, 42(8): 2340-2356. DOI URL
[20]	Zhu C Q, Wei Q Q, Hu W J, Kong Y L, Xiang X J, Zhang H, Cao X C, Zhu L F, Liu J, Tian W H, Jin Q Y, Zhang J H. Unearthing the alleviatory mechanisms of hydrogen sulfide in aluminum toxicity in rice[J]. Plant Physiology and Biochemistry, 2022, 182: 133-144. DOI PMID
[21]	Liu Z Q, Fang H H, Pei Y X, Jin Z P, Zhang L P, Liu D M. WRKY transcription factors down-regulate the expression of H₂S-generating genes, LCD and DES in Arabidopsis thaliana[J]. Science Bulletin, 2015, 60(11): 995-1001. DOI URL
[22]	Kaur H, Hussain S J, Al-Huqail A A, Siddiqui M H, Al-Huqail A A, Khan M I R. Hydrogen sulphide and salicylic acid regulate antioxidant pathway and nutrient balance in mustard plants under cadmium stress[J]. Plant Biology, 2022, 24(4): 660-669. DOI URL
[23]	Singh S, Prasad S M, Singh V P. Additional calcium and sulfur manages hexavalent chromium toxicity in Solanum lycopersicum L. and Solanum melongena L. seedlings by involving nitric oxide[J]. Journal of Hazardous Materials, 2020, 398: 122607. DOI URL
[24]	Husain T, Suhel M, Prasa S M, Singh V P. Ethylene needs endogenous hydrogen sulfide for alleviating hexavalent chromium stress in Vigna mungo L. and Vigna radiata L[J]. Environmental Pollution, 2021, 290: 117968. DOI URL
[25]	Khan M N, Siddiqui M H, AlSolami M A, Alamri S, Hu Y, Ali H M, Al-Amri A A, Alsubaie Q D, Al-Munqedhi B M A, Al-Ghamdi A. Crosstalk of hydrogen sulfide and nitric oxide requires calcium to mitigate impaired photosynthesis under cadmium stress by activating defense mechanisms in Vigna radiata[J]. Plant Physiology and Biochemistry, 2020, 156: 278-290. DOI URL
[26]	Yang J L, Zhu X F, Peng Y X, Zheng C, Li G X, Liu Y, Shi Y Z, Zheng S J. Cell wall hemicellulose contributes significantly to aluminum adsorption and root growth in Arabidopsis[J]. Plant Physiology, 2011, 155(4): 1885-1892. DOI URL
[27]	Xia J X, Yamaji N, Kasai T, Ma J F. Plasma membrane-localized transporter for aluminum in rice[J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107: 18381-18385. DOI PMID
[28]	Hossain M A, Ban K, Hossain A K M Z, Koyama H, Hara T. Combined effects of Mg and Ca supply on alleviation of Al toxicity in wheat plants[J]. Soil Science and Plant Nutrition, 2004, 50: 283-286. DOI URL
[29]	周索, 王绍杰, 余晓丽. 拟南芥花粉细胞质游离钙离子荧光测定法[J]. 生物技术, 2005(5): 55-57.
	Zhou S, Wang S J, Yu X L. Measurement of cytoplasmic calcium fluorescence in Arabidopsis thaliana pollen cells[J]. Biotechnology, 2005(5): 55-57. (in Chinese)
[30]	Dubois M, Gilles K A, Hamilton J K, Rebers P A, Smith F. Colorimetric method for determination of sugars and related substances[J]. Analytical Chemistry, 1956, 28: 350-356. DOI URL
[31]	朱春权, 曹小闯, 朱练峰, 白志刚, 黄洁, 梁清铎, 金千瑜, 张均华. 硫化氢提高水稻磷吸收转运的生理和分子机制[J]. 中国水稻科学, 2019, 33(6): 532-540. DOI
	Zhu C Q, Cao X C, Zhu L F, Bai Z G, Huang J, Liang Q D, Jin Q Y, Zhang J H. Physiological and molecular mechanisms of hydrogen sulfide enhancing phosphorus absorption and transportation in rice[J]. Chinese Journal of Rice Science, 2019, 33 (6): 532-540. DOI
[32]	Blamey F P C, Nishizawa N K, Yoshimura E. Timing, magnitude, and location of initial soluble aluminum injuries to mungbean roots[J]. Soil Science and Plant Nutrition, 2004, 50: 67-76. DOI URL
[33]	Ma J F. Plant root responses to three abundant soil minerals: Silicon, aluminum and iron[J]. Critical Reviews in Plant Sciences, 2005, 24: 267-281. DOI URL
[34]	Hossain M A, Ashrafuzzaman M, Hossain A K, Ismail M R, Koyama H. Role of accumulated calcium in alleviating aluminum injury in wheat plants[J]. Scientific World Journal, 2014: 457187.
[35]	Ligaba-Osena A, Fei Z J, Liu J P, Xu Y M, Shaff Y, Lee S C, Luan S, Kudla J, Kochian L, Pineros M. Loss-of-function mutation of the calcium sensor CBL1 in-creases aluminum sensitivity in Arabidopsis[J]. The New Phytologist, 2017, 214(2): 830-841. DOI URL
[36]	Rudd J J, Franklin-Tong V E. Unravelling response-specificity in Ca²⁺ signalling pathways in plant cells[J]. New Phytologist, 2001, 151(1): 7-33. DOI URL
[37]	Rengel Z, Zhang W H. Role of dynamics of intracellular Calcium in aluminium-toxicity syndrome[J]. New Phytologist, 2003, 159: 295-314. DOI PMID
[38]	Zhang H, Tan Z Q, Hu L Y, Wang S H, Luo J P, Jones R L. Hydrogen sulfide alleviates aluminum toxicity in germinating wheat seedlings[J]. Journal of Integrative Plant Biology, 2010, 52(6): 556-567. DOI
[39]	Clarkson D T. Interactions between aluminium and phosphorus on root surfaces and cell wall material[J]. Plant Soil 1967, 27: 347-356. DOI URL
[40]	Yokosho K, Yamaji N, Kashino-Fujii M, Ma J F. Retrotransposonmediated aluminum tolerance through enhanced expression of the citrate transporter OsFRDL4[J]. Plant Physiology, 2016, 172: 2327-2336. PMID
[41]	Huang C F, Yamaji N, Chen Z, Ma J F. A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice[J]. The Plant Journal, 2011, 69: 857-867. DOI URL

钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制

Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 41

相关文章 15

编辑推荐

Metrics

[1]	廉院训, 韦子芸, 张强, 李清, 任德勇, 胡江, 朱丽, 高振宇, 张光恒, 郭龙彪, 曾大力, 钱前, 沈兰. 水稻斑马叶突变体zl7的鉴定与基因的精细定位[J]. 中国水稻科学, 2023, 37(2): 113-124.
[2]	韦敏益, 马增凤, 黄大辉, 秦媛媛, 刘驰, 卢颖萍, 罗同平, 李振经, 张月雄, 秦钢. 基于QTL-Seq的水稻抗细菌性条斑病QTL定位[J]. 中国水稻科学, 2023, 37(2): 133-141.
[3]	周振玲, 林兵, 周群, 杨波, 刘艳, 周天阳, 王宝祥, 顾骏飞, 徐大勇, 杨建昌. 耐盐性不同水稻品种对盐胁迫的响应及其生理机制[J]. 中国水稻科学, 2023, 37(2): 153-165.
[4]	关雅琪, 鄂志国, 王磊, 申红芳. 浙江省水稻生产环节外包进程的时空演变：基于2010－2019年的跟踪调查[J]. 中国水稻科学, 2023, 37(2): 189-199.
[5]	王炫栋, 余俊杰, 高润杰, 兰赫婷, 江樱姿, 齐文杰, 宋振, 蒋冬花. 一株兼具防病促生功能的沙阿霉素链霉菌Sz-11[J]. 中国水稻科学, 2023, 37(2): 200-212.
[6]	刘淑丽, 张瑞, 王洋, 陈英龙, 韦还和, 侯红燕, 戴其根. 外源物质对水稻盐胁迫缓解效应研究进展[J]. 中国水稻科学, 2023, 37(1): 1-15.
[7]	袁洋, 敖和军, 周仲华, 应杰政, 张健, 倪深. 水稻杂种偏分离位点SegD8的精细定位[J]. 中国水稻科学, 2023, 37(1): 37-44.
[8]	唐杰, 龙湍, 吴春瑜, 李新鹏, 曾翔, 吴永忠, 黄培劲. 水稻光温敏雄性不育突变体tms3650的鉴定和基因定位[J]. 中国水稻科学, 2023, 37(1): 45-54.
[9]	张露, 梁青铎, 吴龙龙, 黄晶, 田仓, 张均华, 曹小闯, 朱春权, 孔亚丽, 金千瑜, 朱练峰. 减氮和增氧灌溉对水稻产量和氮素利用的影响[J]. 中国水稻科学, 2023, 37(1): 78-88.
[10]	巫明明, 曾维, 翟荣荣, 叶靖, 朱国富, 俞法明, 张小明, 叶胜海. 水稻耐盐分子机制与育种研究进展[J]. 中国水稻科学, 2022, 36(6): 551-561.
[11]	李小秀, 吕启明, 袁定阳. OsNramp5基因变异影响水稻重要农艺性状的研究进展[J]. 中国水稻科学, 2022, 36(6): 562-571.
[12]	张元野, 尹丽颖, 李荣田, 何明良, 刘欣欣, 潘婷婷, 田晓杰, 卜庆云, 李秀峰. 利用CRISPR/Cas9技术创制Rc基因恢复红稻[J]. 中国水稻科学, 2022, 36(6): 572-578.
[13]	毛慧, 彭彦, 毛毕刚, 韶也, 郑文杰, 胡黎明, 周凯, 赵炳然. 水稻直链淀粉合成调控新基因Wx410的功能与效应分析[J]. 中国水稻科学, 2022, 36(6): 579-585.
[14]	史玉良, 杨勇, 李雪飞, 李钱峰, 黄李春, 张昌泉, 宋学堂, 刘巧泉. 不同直链淀粉含量软米品种品质性状的比较[J]. 中国水稻科学, 2022, 36(6): 601-610.
[15]	魏晓东, 张亚东, 宋雪梅, 陈涛, 朱镇, 赵庆勇, 赵凌, 路凯, 梁文化, 赫磊, 黄胜东, 谢寅峰, 王才林. 高产粳稻品种南粳晶谷的光合生理特性研究[J]. 中国水稻科学, 2022, 36(6): 611-622.