中国水稻科学 ›› 2022, Vol. 36 ›› Issue (2): 159-170.DOI: 10.16819/j.1001-7216.2021.210204

• 研究报告 • 上一篇    下一篇

利用染色体片段置换系群体定位和分析水稻粒重和粒型QTL

黄涛1,2, 王燕宁1, 钟奇1, 程琴1, 杨朦朦1, 王鹏1, 吴光亮1, 黄诗颖1, 李才敬1, 余剑峰1, 贺浩华1,3,*(), 边建民1,3,*()   

  1. 1作物生理生态与遗传育种教育部重点实验室, 南昌 330045
    2江西农业大学 生物科学与工程学院, 南昌 330045
    3江西省水稻高水平工程研究中心, 南昌 330045
  • 收稿日期:2021-02-06 修回日期:2021-05-02 出版日期:2022-03-10 发布日期:2022-03-11
  • 通讯作者: 贺浩华,边建民
  • 基金资助:
    江西省杰出青年人才训练计划资助项目(20192BCB23010);江西省自然科学类重点项目(20192ACBL20017);江西省重大科技研发专项(20203ABC28W013);大学生创新创业项目(202010410105)

Mapping and Analysis of QTLs for Rice Grain Weight and Grain Shape Using Chromosome Segment Substitution Line Population

HUANG Tao1,2, WANG Yanning1, ZHONG Qi1, CHENG Qin1, YANG Mengmeng1, WANG Peng1, WU Guangliang1, HUANG Shiying1, LI Caijing1, YU Jianfeng1, HE Haohua1,3,*(), BIAN Jianmin1,3,*()   

  1. 1Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang 330045, China
    2College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China
    3Jiangxi Super Rice Engineering Technology Research Center, Nanchang 330045, China
  • Received:2021-02-06 Revised:2021-05-02 Online:2022-03-10 Published:2022-03-11
  • Contact: HE Haohua, BIAN Jianmin

摘要:

【目的】 挖掘水稻粒重和粒型相关性状QTL,对于解析水稻籽粒遗传机理具有重要作用。【方法】 本研究以籼稻9311为受体、粳稻日本晴为供体构建的染色体片段置换系(Chromosome Segment Substitution Lines, CSSLs)群体为材料,在4个环境下对控制稻谷与糙米的粒重和粒型QTL进行了定位分析。【结果】 共检测到77个控制水稻粒重和粒型的QTL,贡献率为4.62%~51.01%,其中19个QTL的增效等位基因来自日本晴,58个QTL的增效等位基因来自9311。这些QTL分布在水稻10条染色体的46个区域,其中16个区域为多效性位点。在两个及两个以上环境中重复检测到的QTL有14个,其中qGW5.1qLW5能够在4个环境中稳定表达,且位于同一染色体区域;qBRL3.2qGL4.1为新鉴定的影响水稻粒重和粒型的QTL。【结论】 本研究结果为后续克隆这些QTL和解析水稻粒重和粒型遗传机理奠定了基础。

关键词: 水稻, 染色体片段置换系, 粒重, 粒型, QTL

Abstract:

【Objective】 Mining quantitative trait loci (QTL) for grain weight and grain shape related traits in rice plays an important role in the genetic mechanism of rice grain.【Method】 QTLs associated with grain weight and grain shape of grain and brown rice were analyzed using the population of chromosome segment substitution lines (CSSLs) with indica rice 9311 as recipient and japonica Nipponbare as donor in four different environments.【Result】 A total of 77 QTLs for grain weight and grain shape of paddy and brown rice were detected, and the phenotypic variation explained by each QTL ranged from 4.62% to 51.01%. Among these QTLs, 19 have additive alleles from Nipponbare and 58 have additive alleles from 9311. These QTLs distributed in 46 regions of 10 chromosomes in rice, and 16 regions are pleiotropic loci. There are 14 QTLs that can be repeatedly detected in two or more environments, qGW5.1 and qLW5 could be repeatedly detected in four environments and were located in the same chromosome region; qBRL3.2 and qGL4.1 were newly identified QTLs affecting grain weight and grain shape in rice.【Conclusion】 The results laid a foundation for cloning these QTLs and analyzing the genetic mechanism of grain shape and grain weight in rice.

Key words: rice, chromosome segment substitution lines (CSSLs), grain weight, grain shape, QTL