中国水稻科学 ›› 2017, Vol. 31 ›› Issue (4): 364-370.DOI: 10.16819/j.1001-7216.2017.7017

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基于重测序的染色体片段代换系定位水稻抽穗期QTL

王军1,2, 朱金燕2, 陶亚军1, 周勇1, 范方军2, 李文奇2, 王芳权2, 仲维功2, 杨杰2,*, 梁国华1,*()   

  1. 1扬州大学 江苏省粮食作物现代产业技术协同创新中心/教育部植物功能基因组学重点实验室, 江苏 扬州 225009
    2江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心, 南京 210014
  • 收稿日期:2017-02-08 修回日期:2017-03-08 出版日期:2017-07-25 发布日期:2017-07-10
  • 通讯作者: 杨杰,梁国华
  • 基金资助:
    国家973计划资助项目(2013CBA01405);公益性行业科研专项(201303102);江苏省现代农业重点研发项目(BE2015355,BE2015341);扬州市农业前瞻性研究资助项目(YZ2014165)

Mapping of QTLs for Heading Date Using Whole-genome Re-sequenced Chromosome Segment Substitution Lines in Rice

Jun WANG1,2, Jinyan ZHU2, Yajun TAO1, Yong ZHOU1, Fangjun FAN2, Wenqi LI2, Fangquan WANG2, Weigong ZHONG2, Jie YANG2,*, Guohua LIANG1,*()   

  1. 1Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Key Laboratory of Plant Function Genomics, Ministry of Education Yangzhou University, Yangzhou 225009, China
    2 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China
  • Received:2017-02-08 Revised:2017-03-08 Online:2017-07-25 Published:2017-07-10
  • Contact: Jie YANG, Guohua LIANG

摘要:

目的水稻的抽穗期是决定水稻产量及其适用性的重要农艺性状之一,是由多基因控制的数量性状。染色体片段代换系减少了个体间遗传背景的干扰,已经成为定位和克隆复杂性状QTL的重要材料。方法本研究以9311为受体,日本晴为供体构建的128个重测序的染色体片段代换系群体为试验材料,利用多元回归,结合Bin-map图谱,【结果】鉴定到6个在南京、扬州不同年份间稳定表达的抽穗期QTL,其中,qHD2.1被定位在第2染色体上的759 848 bp区间内;qHD2.2被定位在第2染色体上的45 286 bp区间内;qHD 3.1被定位在第3染色体上的147 931 bp区间内;qHD5.1被定位在第5染色体上的213 351 bp区间内;qHD5.2被定位在第5染色体上的442 305 bp区间内;qHD8.1被定位在第8染色体上的538 176 bp区间内。结论本研究为精细定位并克隆相应QTL,进而探明抽穗期QTL的分子调控机制奠定了基础。

关键词: 水稻, 染色体片段代换系, 重测序, 抽穗期

Abstract:

【Objective】Heading date is an important agronomic trait in rice, it is a typical quantitative trait controlled by multiple genes. As novel research material, chromosome segment substitution lines are useful in QTL fine mapping and cloning because of minimizing the interference of genetic background among plants. 【Method】In this study, 128 whole-genome re-sequenced chromosome segment substitution lines derived from Nipponbare as donor parent in the background of 9311, were used for mapping QTLs for heading date by combining the sequencing-based Bin-map with multiple linear regression analysis. 【Result】Six QTLs for heading date were identified in two environments and two years. qHD2.1 was mapped in the region of 759 848 bp on the chromosome 2; qHD2.2 was mapped in the region of 45 286 bp on the chromosome 2; qHD 3.1 was mapped in the region of 147 931 bp on the chromosome 3; qHD5.1 was mapped in the region of 213 351 bp on the chromosome 5; qHD5.2 was mapped in the region of 442 305 bp on the chromosome 5; qHD8.1 was mapped in the region of 538 176 bp on the chromosome 8. 【Conclusion】The results are important for the QTLs cloning and provide a foundation for understanding molecular regulation mechanism of heading date in rice.

Key words: rice, chromosome segment substitution lines, re-sequenced, heading date