中国水稻科学 ›› 2016, Vol. 30 ›› Issue (6): 637-646.DOI: 10.16819/j.1001-7216.2016.5178
收稿日期:
2015-12-02
修回日期:
2016-03-09
出版日期:
2016-11-10
发布日期:
2016-11-10
通讯作者:
朱诚
基金资助:
Xu LAN, Zheng-dong GU, Yan-fei DING, Ke WANG, Qiong JIANG, Cheng* ZHU()
Received:
2015-12-02
Revised:
2016-03-09
Online:
2016-11-10
Published:
2016-11-10
摘要:
以野生型水稻中花11及其耐热型突变体(hst)为材料,于抽穗扬花期高温处理7 d (40℃,每天处理6 h),探讨开花期水稻颖花生理特性与耐热性之间的关系。研究表明,花期高温导致剑叶叶绿素含量、颖花可溶性蛋白和可溶性糖含量均显著下降,突变体hst的降幅明显小于野生型中花11;同时,高温下颖花中丙二醛(MDA)、过氧化氢(H2O2)及游离脯氨酸含量均显著增加,而突变体hst的增幅明显低于野生型中花11。在抗氧化酶活性方面,超氧化物歧化酶(SOD)、过氧化物酶(POD)及过氧化氢酶(CAT)活性对水稻开花期高温胁迫的响应有所不同,其变化与耐热性之间的相关性有待进一步验证。高温胁迫下水稻剑叶中保持较稳定的光合作用,颖花中保持较多的渗透调节物质及较低的MDA和H2O2含量,是突变体耐高温的生理基础。
中图分类号:
兰旭, 顾正栋, 丁艳菲, 王珂, 江琼, 朱诚. 花期高温胁迫对水稻颖花生理特性的影响[J]. 中国水稻科学, 2016, 30(6): 637-646.
Xu LAN, Zheng-dong GU, Yan-fei DING, Ke WANG, Qiong JIANG, Cheng* ZHU. Effect of High Temperature Stress on Physiological Characteristics of Spikelet of Rice During Flowering Stage[J]. Chinese Journal OF Rice Science, 2016, 30(6): 637-646.
图1 花期高温胁迫对水稻颖花受精率的影响 ZH11-T-高温胁迫下野生型中花11; hst-T-高温胁迫下突变体; ZH11-CK-适温条件下的野生型中花11; hst-CK-适温条件下的突变体;图中数据为平均数±标准差;n=30;相同字母表示差异未达0.05 显著水平。下同。
Fig. 1. Effect of high temperature stress on the fertilized rate of spikelets at the flowering stage. ZH11-T, Wild type under high temperature stress;hst-T, Mutant under high temperature stress; ZH11-CK, Wild type under natural condition; hst-CK, Mutant under natural condition; All data are mean±standard deviation; n=30; The same letter indicates no significant difference at 0.05 significant level. The same below.
品系 Line | 处理 Treatment | 千粒重 1000-grain weight/g | 每穗实粒数 Grain number per panicle | 单株产量 Yield per plant/g | 结实率 Seed-setting rate/% | 热敏感指数 HSI/% |
---|---|---|---|---|---|---|
中花11 Zhonghua 11 | 适温CK | 23.92±0.02 c | 48.6±5.6 a | 20.94±1.34 b | 68.1±9.5 a | 80.24 |
高温T | 26.41±0.07 a | 10.0±2.5 c | 4.80±0.48 d | 13.4±2.9 c | ||
突变体 hst | 适温CK | 24.09±0.01 b | 57.9±6.0 a | 29.26±1.71 a | 72.8±10.3 a | 46.87 |
高温T | 24.19±0.09 b | 27.1±3.7 b | 13.79±0.99 c | 38.4±4.8 b |
表1 高温胁迫对水稻结实率、千粒重及单株产量的影响
Table 1 Effect of high temperature stress on rice seed-setting rate,1000-grain weight and yield per plant.
品系 Line | 处理 Treatment | 千粒重 1000-grain weight/g | 每穗实粒数 Grain number per panicle | 单株产量 Yield per plant/g | 结实率 Seed-setting rate/% | 热敏感指数 HSI/% |
---|---|---|---|---|---|---|
中花11 Zhonghua 11 | 适温CK | 23.92±0.02 c | 48.6±5.6 a | 20.94±1.34 b | 68.1±9.5 a | 80.24 |
高温T | 26.41±0.07 a | 10.0±2.5 c | 4.80±0.48 d | 13.4±2.9 c | ||
突变体 hst | 适温CK | 24.09±0.01 b | 57.9±6.0 a | 29.26±1.71 a | 72.8±10.3 a | 46.87 |
高温T | 24.19±0.09 b | 27.1±3.7 b | 13.79±0.99 c | 38.4±4.8 b |
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