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    Reviews and Special Topics
    Advances in Research on the Modification of the Heading Date Genes in Rice by Phosphorylation and Ubiquitination Pathways
    WANG Jingying, ZHAO Guangxin, QIU Guankai, FANG Jun
    2022, 36(3): 215-226.  DOI: 10.16819/j.1001-7216.2022.210707
    Abstract ( )   HTML ( )   PDF (1000KB) ( )  

    Rice is a facultative short-day plant that is widely cultivated. Heading date (also known as flowering time) of rice is an important agronomic trait that directly affects grain yield and regional adaptability of rice varieties. Therefore, it is of great significance to study the influencing factors of this trait and make the plants bloom at appropriate time. Heading date, as a complex quantitative trait, is regulated by internal genetic network and exogenous factors such as photoperiod and temperature. At present, several key genes controlling heading date have been identified and cloned. It is found that phosphorylation and ubiquitylation play an important role in the molecular mechanism of rice heading date. This paper introduces the molecular mechanism of photoperiod pathway of heading date, and expounds the progress in research on the regulation of phosphorylation cascade and ubiquitin 26S proteasome system on flowering time, aiming at laying a theoretical basis and providing practical guidance for the new mechanism in regulating heading date, and for regional adaptability of improved varieties.

    Progress in Functions, Allelic Variations and Interactions of Soluble Starch Synthases Genes SSⅡa and SSⅢa in Rice
    YAO Shu, ZHANG Yadong, LU Kai, WANG Cailin
    2022, 36(3): 227-236.  DOI: 10.16819/j.1001-7216.2022.210705
    Abstract ( )   HTML ( )   PDF (5007KB) ( )  

    The effect of rice starch synthesis on rice quality has always been under the spotlight, which is one of the hotspots and difficult points for rice basic scientific research. The pathway of starch synthesis is regulated by many kinds of enzymes, but soluble starch synthase (SSS) is one of the most important enzymes influencing rice eating and cooking quality. Soluble starch synthase genes SSⅡa and SSⅢa are two key genes controlling rice gelatinization temperature, which are involved in biosynthesis of amylopectin of rice endosperm. We reviewed and summarized the latest research progress in the functions, allelic variations and interactions of SSⅡa and SSⅢa affecting cooking and eating quality. Meanwhile, we discussed the application perspective of SSⅡa and SSⅢa in molecular improvement and breeding of rice quality.

    Research Papers
    Construction of osarf7 Mutants in Rice Based on CRISPR/Cas9 Technology and Investigation on Their Agronomic Traits
    LI Zhaowei, SUN Congying, LING Donglan, ZENG Huiling, ZHANG Xiaomei, FAN Kai, LIN Wenxiong
    2022, 36(3): 237-247.  DOI: 10.16819/j.1001-7216.2022.210117
    Abstract ( )   HTML ( )   PDF (109895KB) ( )  

    【Objective】The purpose is to offer a deep insight into the biological function of OsARF7 and its effect on agronomic traits during the growth and development stage of rice. 【Method】CRISPR/Cas9 technology was employed to edit the gene sequence of OsARF7 and to obtain the mutants of japonica variety Zhonghua 11. The agronomic traits of the mutants were investigated in a field experiment. 【Result】Twenty-two T0 transgenic lines were obtained through Agrobacterium-mediated infection of japonica rice Zhonghua 11 callus, and twenty positive transgenic lines were distinguished by screening the gene encoding hygromycin. For T2 transgenic lines, the regions of genomic DNA including two target sites were detected and identified by PCR amplifying and sequencing, and fifteen homozygous mutations were confirmed. Gene expression results displayed that the expression levels of OsARF7, OsPIN1b, OsPIN2, and OsLAX2 in the osarf7 mutants were significantly lower than those in the wild type. In comparison with the wild-type plants, osarf7 mutants showed a significant decrease in the productive panicle percentage. Among them, the osarf7 mutants with more tillers than the wild type showed a similar effective panicle number to the wild type, and the osarf7 mutants with the same tiller number as the wild type presented a decrease in the effective panicle number per plant in paddy field conditions, resulting in increased non-productive tillers and decreased effective panicle percentage. 【Conclusion】OsARF7 was involved in the emergence and development of tiller bud dominated by auxin, and the successful construction of osarf7 mutants plays a positive role in revealing the biological function of OsARF7 and the mechanism of tiller formation and development dominated by auxin.

    Developing Fragrant Early indica TGMS Line with Blast Resistance by Using CRISPR/Cas9 Technology
    LIANG Minmin, ZHANG Huali, CHEN Junyu, DAI Dongqing, DU Chengxing, WANG Huimei, MA Liangyong
    2022, 36(3): 248-258.  DOI: 10.16819/j.1001-7216.2022.211007
    Abstract ( )   HTML ( )   PDF (18446KB) ( )  

    【Objective】 New thermosensitive genic male sterile lines with high resistance and fragrance quality will provide resources for hybrid rice breeding. 【Method】The target genes Pi21, TMS5 and Badh2 were selected to construct the pC1300-2×35S::gTMS5-gBadh2-gPi21expression vector by CRISPR/Cas9 technology for transformation of early indica rice Zhongzao 70. The positive transgenic plants were obtained through sequencing analysis. Magnaporthe oryzae spraying inoculation and punching inoculation assays were conducted to identify the resistance to rice blast, and the content of 2-acetyl-1-pyrroline was measured by GC-MS. 【Result】In T0 transgenic lines, the mutation rates of Pi21, TMS5 and Badh2 were 87.5%, 80.0% and 87.5%, respectively, and most of the mutant lines are biallelic mutations. Two types of the homozygous triple mutants without the vector were obtained in T1 transgenic lines. Evaluation analysis of resistance to rice blast suggested that T2 generation of the pi21 homozygous mutant lines showed significantly higher resistance to M. oryzae compared with the wild type. The expression levels of defense-related genes were up-regulated and the ROS accumulation increased in the homozygous mutant lines compared to the wild type after inoculation of M. oryzae. tms5 homozygous mutant lines displayed thermo-sensitive male sterility. Compared with the wild type, the expression level of TMS5 was significantly decreased and the transcription of UbL404 was increased in the tms5 homozygous mutants at high temperature. The expression level of Badh2 was down-regulated and the content of 2-AP increased dramatically in the Badh2 homozygous mutant lines. 【Conclusion】The homozygous triple mutant lines of Pi21, TMS5 and Badh2 were obtained by CRISPR/Cas9-mediated technology, which would provide materials for high-resistant and fragrant rice breeding.

    Screening and QTL Mapping of Heat-tolerant Rice (Oryza sativa L.) Germplasm Resources at Seedling Stage
    LIU Jin, CUI Di, YU Liqin, ZHANG Lina, ZHOU Huiying, MA Xiaoding, HU Jiaxiao, HAN Bing, HAN Longzhi, LI Maomao
    2022, 36(3): 259-268.  DOI: 10.16819/j.1001-7216.2022
    Abstract ( )   HTML ( )   PDF (201160KB) ( )  

    【Objective】Identification of germplasm resources and genes extremely resistant to heat will provide technical support and lay a physical foundation for the breeding of new heat-tolerant rice varieties. 【Method】Heat tolerance level and survival rate are used to identify the high temperature resistance of rice at seedling stage, so as to select heat-tolerant germplasm resources and identify major QTLs. 【Results】There are obvious differences in heat tolerance among different varieties at seedling stage. Indica rice has significantly stronger tolerance to heat than japonica rice. Twenty accessions of rice germplasm resources with strong heat tolerance, such as Jiayu 253, Zhongyouzao 8, and Xiushui 09, were selected. The survival rates, growth and development of these varieties were almost immune to high temperature. Furthermore, there are significant differences in heat tolerance between the parents. Zhongyouzao 8 has strong heat resistance, while Longdao 5 is sensitive to high temperature. The phenotype followed an approximate continuous normal distribution under high temperature in the RIL populations. Twelve QTLs were detected on chromosomes 1, 3, 4, 5 and 8, and these QTLs have a significant genetic overlap for heat resistance grade(HTG) and survival rate under heat stress(HTSR). The major QTL clusters qHTS4 and qHTS8 have significant heat tolerance function. Based on the preliminary QTL mapping results, the RHL-F2 population was used to validate the stably expressed major QTL qHTS8, which was identified on the chromosome 8 between RM5808 and RM556 regions. 【Conclusion】 Twenty accessions of rice germplasm resources with strong heat tolerance were selected, twelve QTLs were identified, and then the major QTL qHTS8 was verified to have strong effects on regulating heat tolerance. These results will lay a foundation and provide abundant germplasm resources for the research on the physiological, biochemical, and molecular genetic mechanism of heat resistance and rice breeding.

    Effects of Alternate Wetting and Moderate Soil Drying Irrigation on Root Traits, Grain Yield and Soil Properties in Rice
    CHEN Yun, LIU Kun, LI Tingting, LI Siyu, LI Guoming, ZHANG Weiyang, ZHANG Hao, GU Junfei, LIU Lijun, YANG Jianchang
    2022, 36(3): 269-277.  DOI: 10.16819/j.1001-7216.2022.210309
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    【Objective】 Alternate wetting and moderate soil drying irrigation (WMD) plays important roles in root growth and yield formation in rice. However, its effect on soil properties and their relationship with root growth is not clearly understood. 【Method】 In this experiment, the effects of continuously flooded irrigation (CI) and WMD during the grain filling stage on rice yield, root morphology and physiology and soil properties were studied using five different types of rice varieties. 【Result】 1) Compared with CI, WMD during the grain filling stage could significantly increase the seed-setting rate and 1000-grain weight of different varieties, thereby increase the grain yield in rice. 2) The nitrate nitrogen content, the activities of urease and sucrase in the rhizosphere and non-rhizosphere soils could be increased, and the soil ammonium nitrogen content could be reduced after rewatering under WMD during the grain filling stage. 3) The rice root morphology (root dry weight, root number, root length, root surface, root volume, root aerenchyma area) and root activity (root oxidation activity) after rewatering with WMD during the grain filling stage were significantly higher than those in CI treatment. 【Conclusion】 Greater nitrate nitrogen contents, higher activities of urease and sucrase and lower ammonium nitrogen contents in rhizosphere and non-rhizosphere soils after WMD rewatering could improve root morphological and physiological traits, promote grain filling, and increase rice yield finally.

    Effects of Nitrogen Reduction Model on Yield and Nitrogen Absorption and Utilization of Late-maturing Mid-japonica Rice with Different Panicle Types
    ZHANG Xiaoxiang, SHAO Shimei, ZHAO Buhong, ZHANG Hao, JI Hongjuan, XIAO Ning, PAN Cunhong, LI Yuhong, WU Yunyu, CAI Yue, LIU Jianju, JI Chunming, ZHANG Xiuqin, LIU Guangqing, ZHOU Changhai, HUANG Niansheng, LI Aihong
    2022, 36(3): 278-294.  DOI: 10.16819/j.1001-7216.2022.211003
    Abstract ( )   HTML ( )   PDF (787KB) ( )  

    【Objective】The purpose of this study is to clarify the effects of the key period of reduced nitrogen application and appropriate reduced application rate on the yield and nitrogen absorption and utilization efficiency of late-maturing mid-japonica rice with different panicle types. 【Method】Using large panicle cultivar (Fengjing 3227, FJ3227), medium panicle cultivar (Huidao 5, HD5) and small panicle cultivar (Yangfujing 8, YFJ8) as materials, 12 nitrogen fertilizer treatments were designed, namely zero nitrogen application (0N), local conventional nitrogen application (CN, no nitrogen reduction, 300 kg/hm2), basal nitrogen fertilizer reduction by 10% and 20% (BN10, BN20), nitrogen reduction by 10% and 20% in tillering fertilizer(TN10, TN20), nitrogen reduction by 10% and 20% in flower-promoting fertilizer (SPN10, SPN20), nitrogen reduction by 10% and 20% in flower-preserving fertilizer (SNN10, SNN20) and average nitrogen reduction of 10% and 20% (AN10, AN20), to study the effects of different nitrogen reduction modes on yield and nitrogen absorption and utilization of medium-maturing japonica rice varieties differed in panicle type. 【Result】Under reduced nitrogen treatment, the overall yield of FJ3227, HD5 and YFJ8 decreased by 6.17%, 7.67% and 7.81%, respectively, with greater decrease in 20% nitrogen reduction. For FJ3227, HD5 and YFJ8 under the treatment of SPN10, AN10 and SSN10, the yield dropped by 0.17%, 0.79% and 0.23%, insignificantly lower than CN. Under the same nitrogen reduction model, the highest yield decline was observed in small panicle variety, followed by medium panicle type variety, and large panicle type variety. Nitrogen reduction reduced the number of effective panicles and the number of grains per panicle, but increased the seed setting rate and 1000-grain weight. As a result of nitrogen reduction, the number of effective panicles had the greatest promotion effect on the yield of the large panicle cultivar FJ3227, while the number of grains per panicle contributed the most to the yield of the medium panicle cultivar HD5 and the small panicle cultivar YFJ8. Nitrogen reduction treatment reduced the amount of nitrogen transportation and the amount of nitrogen accumulation. And the nitrogen reduction treatments reduced the nitrogen uptake at the jointing stage, heading stage and maturity stage. There were significant differences between treatments and varieties. Nitrogen reduction improved nitrogen fertilizer absorption and utilization and nitrogen partial productivity. Under the nitrogen reduction mode, the large panicle cultivar FJ3227, the medium panicle cultivar HD5, and the small panicle cultivar YFJ8 under the treatments of SPN10, AN10, and SSN10 have higher nitrogen use efficiency. 【Conclusion】The large panicle variety can reduce nitrogen by appropriate amount of fertilizer for promoting flowers, the small panicle variety can reduce nitrogen by medium amount of flower-preserving fertilizer, and the medium panicle variety can achieve nitrogen reduction without reducing yield and improve nitrogen absorption and utilization. Medium nitrogen reduction can adjust the growth characteristics of different panicle rice populations to achieve stable yield. Yet the excessive nitrogen reduction weakens its yield regulation capacity.

    Effects of Different Nitrogen Application Regimes on Translocation of Rice Photosynthetic Products and Nitrogen Under Alternate Wetting and Drying Irrigation
    WU Longlong, YU Yijun, TIAN Cang, ZHANG Lu, HUANG Jing, ZHU Lianfeng, ZHU Chunquan, KONG Yali, ZHANG Junhua, CAO Xiaochuang, JIN Qianyu
    2022, 36(3): 295-307.  DOI: 10.16819/j.1001-7216.2022.210507
    Abstract ( )   HTML ( )   PDF (15116KB) ( )  

    【Objective】Field plot experiments were conducted to study the effects of different irrigation and nitrogen application regimes on yield, photosynthetic physiological characteristics, non-structural carbohydrate and nitrogen use efficiency of rice, so as to lay a theoretical basis for scientific and reasonable irrigation and nitrogen management in local paddy fields. 【Method】With Zhongzheyou 1 as material, a field experiment was carried out under two irrigation regimes of flooding irrigation (FI) and alternate wetting and drying (AWD), as well as five nitrogen application regimes of zero nitrogen (N0), conventional nitrogen application (PUN100), 20% nitrogen reduction (PUN80), 20% nitrogen reduction of slow-release compound fertilizer with biochar (CRFN80-BC) and 20% nitrogen reduction of stable compound fertilizer with biochar (SFN80-BC). 【Result】Different irrigation and nitrogen application regimes significantly affected the grain yield and nitrogen use efficiency, and the two factors showed significant interaction. Compared with FI, CRFN80-BC and SFN80-BC treatments significantly increased the net photosynthetic rate and leaf area index of rice at full heading stage, leaf non-structural carbohydrate accumulation and its transfer to grains under AWD, and finally increased the number of effective panicles and grains per panicle of rice. Compared with PUN100, the yield increased by 6.8% and 10.4%, and the average yield in two years was 9656.5 kg/hm2 and 10033 kg/hm2, respectively. AWD also increased the amount of N translocation and the contribution rate of N translocation of stem and leaves from heading to filling stages under CRFN80-BC and SFN80-BC treatments, and then significantly increased rice nitrogen use efficiencies. Compared with PUN80, CRFN80-BC and SFN80-BC under AWD significantly increased N recovery efficiency, N agronomic efficiency and N partial productivity, by 37.8% and 58.4%, 56.6% and 71.1%, 15.2% and 19.3%, respectively. 【Conclusion】Under AWD regimes, 20% (144 kg/hm2) N reduction of the stable compound fertilizer or slow-release compound fertilizer combined with biochar treatment significantly promote the leaf photosynthetic rate, non-structural carbohydrate and nitrogen accumulation at the vegetative growth period of rice, as well as their transportation to panicles at the reproductive growth period, which jointly improve rice yield and nitrogen use efficiency. It can serve as the suitable water and nitrogen management regimes for the green and efficient rice cultivation at the local area.

    Monitoring Rice Leaf Area Index Based on Unmanned Aerial Vehicle (UAV) Digital Images
    CAO Zhongsheng, LI Yanda, HUANG Junbao, YE Chun, SUN Binfeng, SHU Shifu, ZHU Yan, HE Yong
    2022, 36(3): 308-317.  DOI: 10.16819/j.1001-7216.2022.210712
    Abstract ( )   HTML ( )   PDF (2982KB) ( )  

    【Obiective】Leaf area index (LAI) is a crucial variable for assessing rice growth, and unmanned aerial vehicle (UAV) digital images can serve as an efficient way to real-time, no-destructive monitoring of crop growth parameters. However, it remains unclear which parameter in digital images can be used to estimate rice LAI. In addition, the optimal growth stage for monitoring is also unknown. 【Method】In this study, the UAV digital images were initially collected from two field experiments encompassing variations over two years with four cultivars at four nitrogen application levels. Then, the relationship between UAV digital image parameters (nine color indices and eight texture features) and rice LAI at different growth stages (tillering stage, jointing stage, booting stage, heading stage and filling stage) were analyzed. 【Result】The results suggested that the early growth stages, including both tillering stage and jointing stage, were suitable for rice LAI monitoring through UAV digital images, and the texture feature variance (VAR) exhibits greatest accuracy in model calibration with a determination coefficient (R2) of 0.7980. In the validation based on independent experiment, this texture feature also performs well with relative root mean square error (RRMSE) of 0.1658 and bias(θ) of 0.1306. 【Conclusion】Taking the accuracy and convenience in application into consideration, we found that the texture feature VAR could be used to monitor rice LAI in early growth stage with estimation models of LAI = 1.1656×exp(0.0174×VAR).

    Effects of Temperature on Biological Traits of the Pincer Wasp [Gonatopus flavifemur (Esaki & Hashimoto)], a Natural Enemy of the Brown Planthopper(Nilaparvata lugens)
    HE Jiachun, HE Yuting, WAN Pinjun, WEI Qi, LAI Fengxiang, CHEN Xiangsheng, FU Qiang
    2022, 36(3): 318-326.  DOI: 10.16819/j.1001-7216.2022.210605
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    【Objective】 Gonatopus flavifemur (Esaki & Hashimoto) is a common parasitoid against Nilaparvata lugens (Stål) in southern China. This research aims to reveal the effects of temperature on biological traits of G. flavifemur reared on N. lugens. 【Methods】 The longevity, parasitic rates, host-feeding rates of parental generation and cocooning rate, emergence rates, developmental duration, sexual ratio of their F1 offspring were investigated at seven temperatures (12, 17, 22, 27, 32, 36 and 38℃) in laboratory. In addition, the population parameters at different temperatures and the developmental threshold temperature, effective accumulative temperature for one generation were estimated. 【Result】1) The temperature had great effects on all measured biological traits. The longevity of both males and females showed negative relation with increasing temperatures ranging from 17℃ to 38℃. Male and female adults could not survive for more than 1 d at 38℃. The highest parasitic rate (52.3%) and the highest host-feeding rate (17.7%) were observed at 32℃. These two parameters showed no significant difference between 27℃ and 32℃. But the parasitic rate and host-feeding rate at 38℃ were all recorded as zero. The offspring of G. flavifemur could cocoon and emerge between 17 and 36℃. The highest cocooning rate and emergence rate were observed at 27℃. The highest proportion of females in offspring was 28.6% at 32℃ and the highest number of female offspring was 30.5 at 27℃. The developmental duration of offspring was negatively related to the increasing temperature in the range of 17–36℃. 2) The highest intrinsic increase rate was 0.52 at 27℃, followed by 32℃ and 22℃. The intrinsic increase rate was lowest at 17℃. 3) The developmental threshold temperature of males and females were 11.9℃ and 12.0℃, respectively. The generation effective accumulative temperature of males and females were 337.6 d·℃ and 343.8 d·℃, respectively. 【Conclusion】 G. flavifemur could survive in the temperature range of 17–36 ℃,the suitable temperature for offspring development and population growth ranged from 27℃ to 32℃, and 27℃ was the most suitable temperature. These results will lay an important foundation for study on G. flavifemur for mass reproduction of this wasp in the future.