Chinese Journal of Rice Science

• 实验技术 • Previous Articles     Next Articles

One-Step PCR Method for Detecting the First Base of Splice Donor of Wx Intron 1 in Rice

MAO Xing-xue , XIAO Xin , CHEN Jian-wei , LUO Wen-yong , LIU Yan-zhuo , LI Xiao-fang   

  • Received:1900-01-01 Revised:1900-01-01 Online:2005-05-10 Published:2005-05-10

PCR一步法检测水稻蜡质基因第一内含子剪接供体+1位碱基

毛兴学;肖昕;陈建伟;罗文永;刘彦卓;李晓方   

  1. 广东省农业科学院 水稻研究所, 广东省水稻育种新技术重点实验室, 广东 广州 510640; *通讯联系人, Email:lixiaofang38@263.com

Abstract: A new method to detect the first base of splice donor of Wx intron 1 in rice was reported, and some indica rice samples from Guangdong Province were analyzed using this method. The results showed that this new method is credible, easier and cheaper than the usual PCR-Acc Ⅰ method. It meets the requirement for amylose content selection in rice breeding.

Key words: rice, amylose content, polymerase chain reaction, research methodology

摘要: 报道了一种利用PCR快速检测水稻蜡质基因第一内含子剪接供体+1位碱基的方法,并用这种方法分析了一批广东籼稻材料。结果显示,该方法简便易行,结果可靠,可广泛用于育种筛选中的直链淀粉含量选择。

关键词: 水稻, 直链淀粉含量, 聚合酶链式反应, 研究方法