Chinese Journal of Rice Science

• 研究报告 • Previous Articles     Next Articles

Sensitivity of Plant Height Genes to GA3 and Their Relationship with Enzymes in Rice

He Zuhua; Etoh Kossi; Shi Chunhai; Shen Zongtan   

  • Received:1900-01-01 Revised:1900-01-01 Online:1993-07-10 Published:1993-07-10

水稻株高基因对GA3敏感性及与酶的关系

何祖华;Etoh Kossi;石春海;申宗坦   

  1. 浙江农业大学,杭州310029

Abstract: Sensitivities of different height genes of rice to GA3 and their relationships with enzymes were studied using three pairs of isogenic lines. The results indicated that the sensitivities of the genes to GA3 were in the order: eui>sd1>Sd1>sds(t). The gene sd1was more sensitive in indica than in japonica. Sensitivities of the endosperms with the different height genes to GA3 quitely accorded with these of the seedlings. Assay of peroxidase showed that activity of the enzyme was higher in the tall lines than in the short lines in seedlings of 4 days, and higher in the short lines than in the tall ones in seedlings of 8 days. The enzyme was lower activity in sensitive lines treated with GA3 for 4 and 8 days. The isozyme band 8 of peroxidase and the band 7 of esterase on the electrophoresis gel were related to the sensitivity of the height genes to GA3.

Key words: Rice, Plant height genes, Sensitivity to GA3, Enzyme

摘要: 用3对水稻植株高度近等基因对,研究了株高基因对GA3 敏感性和酶的影响。结果表明,株高基因在苗期对GA3 敏感性强弱顺序为eui>sd1>Sd1>sds(t);其中梗稻的Sd1敏感性强于籼稻的。 用胚乳培养法快速鉴别水稻株高基因对GA3敏感性的结果与幼苗鉴定的一致,说明这种方法是有效的。 在酶活性方面,4 d的幼苗中高秆品系的过氧化物酶活力比矮秆品系的高, 而8 d的幼苗中矮秆的活力反而比高秆的强。经GA3处理4 d和8 d后,对GA3敏感的品系过氧化物酶活力较低, 过氧化物酶同工酶带8和酯酶同工酶带7与株高对GA3的敏感性有关。

关键词: 水稻, 株高基因, 敏感性, 酶, 赤霉素