Chinese Journal OF Rice Science ›› 2024, Vol. 38 ›› Issue (6): 709-718.DOI: 10.16819/j.1001-7216.2024.240310
• Research Papers • Previous Articles
YAO Shu, CHEN Tao, ZHAO Chunfang, ZHOU Lihui, ZHAO Ling, LIANG Wenhua, HAO Lei, LU Kai, ZHU Zhen, ZHAO Qingyong, GUAN Ju, WANG Cailin, ZHANG Yadong*()
Received:
2024-03-26
Revised:
2024-05-09
Online:
2024-11-10
Published:
2024-11-15
Contact:
*email: ydzhang@jaas.ac.cn
姚姝, 陈涛, 赵春芳, 周丽慧, 赵凌, 梁文化, 赫磊, 路凯, 朱镇, 赵庆勇, 管菊, 王才林, 张亚东*()
通讯作者:
*email: ydzhang@jaas.ac.cn
基金资助:
YAO Shu, CHEN Tao, ZHAO Chunfang, ZHOU Lihui, ZHAO Ling, LIANG Wenhua, HAO Lei, LU Kai, ZHU Zhen, ZHAO Qingyong, GUAN Ju, WANG Cailin, ZHANG Yadong. Analysis on Appearance and Cooking Taste Quality Characteristics of Different Types of japonica Rice in Jianghuai Rice-growing Area[J]. Chinese Journal OF Rice Science, 2024, 38(6): 709-718.
姚姝, 陈涛, 赵春芳, 周丽慧, 赵凌, 梁文化, 赫磊, 路凯, 朱镇, 赵庆勇, 管菊, 王才林, 张亚东. 江淮稻区不同类型粳稻品种外观及蒸煮食味品质特征比较[J]. 中国水稻科学, 2024, 38(6): 709-718.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2024.240310
引物序列 Primer sequence | 产物大小 Product size (bp) | 退火温度 Annealing temperature(℃) |
---|---|---|
Wx-F: GGCTGTAAGCACACACAAACTTC | 377 377 | 61 61 |
Wxb -R: GAACACACGGTCGACTCaAC | ||
Wxmp -R: GAACACACGGTCGACTCaAT |
Table 1. Wx allele-specific PCR marker detection primers
引物序列 Primer sequence | 产物大小 Product size (bp) | 退火温度 Annealing temperature(℃) |
---|---|---|
Wx-F: GGCTGTAAGCACACACAAACTTC | 377 377 | 61 61 |
Wxb -R: GAACACACGGTCGACTCaAC | ||
Wxmp -R: GAACACACGGTCGACTCaAT |
Fig. 1. PCR amplification products by using AS-PCR A, Wxmp amplification product; B, Wxb amplification product. Lanes 1-13 represented semi-waxy japonica rice varieties, i.e. Huruan 1212, Nanjing 46, Nanjing 5055, Nanjing 3908, Nanjing 9108, Nanjing 5718, Changxiangjing 1813, Jinxiangyu 1, Ningxiangjing 9, Yangjing 5118, Yangnongxiang 28, Xudao 9, Suxiangjing 3, respectively. Lanes 14-26 represented common japonica rice varieties, i.e. Nanjing 5758, Nanjingqinggu, Wankenjing 3, Wuyujing 30, Suken 118, Wuxiangjing 7368, Huaidao 5, Wuyujing 39, Suxiu 867, Sidao 301, Xudao 15, Lianjing 18, Yanjing 15, respectively.
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV (%) |
---|---|---|---|---|
整精米粒长 Length of polished rice (mm) | 半糯型粳稻SJ | 4.31~4.95 | 4.62±0.18 a | 3.89 |
普通粳稻CJ | 4.10~5.49 | 4.77±0.36 a | 7.54 | |
整精米粒宽 Width of polished rice (mm) | 半糯型粳稻SJ | 2.24~2.93 | 2.68±0.17 a | 6.43 |
普通粳稻CJ | 2.49~2.75 | 2.63±0.07 a | 2.68 | |
长宽比 Length-width ratio of polished rice | 半糯型粳稻SJ | 1.61~1.92 | 1.73±0.10 b | 5.66 |
普通粳稻CJ | 1.57~2.10 | 1.82±0.14 a | 7.73 | |
垩白率Chalky rice percentage (%) | 半糯型粳稻SJ | 10.85~70.17 | 34.82±20.09 a | 57.70 |
普通粳稻CJ | 4.45~54.80 | 18.01±13.82 b | 76.73 | |
垩白度Chalkiness degree (%) | 半糯型粳稻SJ | 1.80~28.38 | 12.72±8.91 a | 70.02 |
普通粳稻CJ | 1.06~20.16 | 6.41±5.70 b | 89.04 | |
透明度Rice transparency | 半糯型粳稻SJ | 1~4 | 2.54±1.02 a | 40.26 |
普通粳稻CJ | 1~3 | 1.38±0.63 b | 45.72 |
Table 2. Differences in grain type and appearance quality of different types of japonica rice varieties
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV (%) |
---|---|---|---|---|
整精米粒长 Length of polished rice (mm) | 半糯型粳稻SJ | 4.31~4.95 | 4.62±0.18 a | 3.89 |
普通粳稻CJ | 4.10~5.49 | 4.77±0.36 a | 7.54 | |
整精米粒宽 Width of polished rice (mm) | 半糯型粳稻SJ | 2.24~2.93 | 2.68±0.17 a | 6.43 |
普通粳稻CJ | 2.49~2.75 | 2.63±0.07 a | 2.68 | |
长宽比 Length-width ratio of polished rice | 半糯型粳稻SJ | 1.61~1.92 | 1.73±0.10 b | 5.66 |
普通粳稻CJ | 1.57~2.10 | 1.82±0.14 a | 7.73 | |
垩白率Chalky rice percentage (%) | 半糯型粳稻SJ | 10.85~70.17 | 34.82±20.09 a | 57.70 |
普通粳稻CJ | 4.45~54.80 | 18.01±13.82 b | 76.73 | |
垩白度Chalkiness degree (%) | 半糯型粳稻SJ | 1.80~28.38 | 12.72±8.91 a | 70.02 |
普通粳稻CJ | 1.06~20.16 | 6.41±5.70 b | 89.04 | |
透明度Rice transparency | 半糯型粳稻SJ | 1~4 | 2.54±1.02 a | 40.26 |
普通粳稻CJ | 1~3 | 1.38±0.63 b | 45.72 |
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准 Mean±SD | 变异系数 CV(%) |
---|---|---|---|---|
蛋白质含量Protein content(%) | 半糯型粳稻SJ | 5.92~7.91 | 6.69±0.48 a | 7.18 |
普通粳稻CJ | 6.07~7.21 | 6.48±0.30 a | 4.62 | |
直链淀粉含量Amylose content(%) | 半糯型粳稻SJ | 7.23~9.96 | 8.54±0.81 b | 9.43 |
普通粳稻CJ | 14.95~17.77 | 15.33±2.05 a | 4.67 | |
胶稠度Gel consistency(mm) | 半糯型粳稻SJ | 71~96 | 85.00±4.94 a | 5.81 |
普通粳稻CJ | 64~81 | 74.21±4.89 b | 6.59 | |
糊化温度Gelatinization temperature(℃) | 半糯型粳稻SJ | 71.23~74.35 | 72.78±0.77 b | 1.06 |
普通粳稻CJ | 71.95~78.20 | 74.67±1.99 a | 2.67 |
Table 3. Differences in starch physicochemical properties of different types of japonica rice varieties
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准 Mean±SD | 变异系数 CV(%) |
---|---|---|---|---|
蛋白质含量Protein content(%) | 半糯型粳稻SJ | 5.92~7.91 | 6.69±0.48 a | 7.18 |
普通粳稻CJ | 6.07~7.21 | 6.48±0.30 a | 4.62 | |
直链淀粉含量Amylose content(%) | 半糯型粳稻SJ | 7.23~9.96 | 8.54±0.81 b | 9.43 |
普通粳稻CJ | 14.95~17.77 | 15.33±2.05 a | 4.67 | |
胶稠度Gel consistency(mm) | 半糯型粳稻SJ | 71~96 | 85.00±4.94 a | 5.81 |
普通粳稻CJ | 64~81 | 74.21±4.89 b | 6.59 | |
糊化温度Gelatinization temperature(℃) | 半糯型粳稻SJ | 71.23~74.35 | 72.78±0.77 b | 1.06 |
普通粳稻CJ | 71.95~78.20 | 74.67±1.99 a | 2.67 |
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV(%) |
---|---|---|---|---|
峰值黏度Peak viscosity (cP) | 半糯型粳稻SJ | 2555 ~ 3248 | 2837.85±175.63 a | 6.19 |
普通粳稻CJ | 1509 ~ 2826 | 2388.46±313.03 b | 13.11 | |
热浆黏度Trough viscosity (cP) | 半糯型粳稻SJ | 697 ~ 2019 | 1387.77±275.64 b | 19.86 |
普通粳稻CJ | 1023 ~ 2264 | 1739.12±340.26 a | 19.75 | |
最终黏度Final viscosity (cP) | 半糯型粳稻SJ | 1007 ~ 2619 | 1986.35±336.49 b | 16.94 |
普通粳稻CJ | 1313 ~ 3180 | 2488.31±565.37 a | 22.72 | |
崩解值Breakdown (cP) | 半糯型粳稻SJ | 921 ~ 1667 | 1215.62±212.18 a | 17.45 |
普通粳稻CJ | 478 ~ 960 | 792.5±130.79 b | 16.50 | |
消减值Setback (cP) | 半糯型粳稻SJ | −864 ~ −186 | −477.69±188.43 b | 39.45 |
普通粳稻CJ | −1279 ~ −186 | −541.81±265.91 a | 49.08 | |
回复值Consistence (cP) | 半糯型粳稻SJ | 310 ~ 683 | 560.96±81.94 b | 14.61 |
普通粳稻CJ | 140 ~ 1003 | 780.35±255.16 a | 32.70 |
Table 4. Differences in starch physicochemical properties of different types of japonica rice varieties
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV(%) |
---|---|---|---|---|
峰值黏度Peak viscosity (cP) | 半糯型粳稻SJ | 2555 ~ 3248 | 2837.85±175.63 a | 6.19 |
普通粳稻CJ | 1509 ~ 2826 | 2388.46±313.03 b | 13.11 | |
热浆黏度Trough viscosity (cP) | 半糯型粳稻SJ | 697 ~ 2019 | 1387.77±275.64 b | 19.86 |
普通粳稻CJ | 1023 ~ 2264 | 1739.12±340.26 a | 19.75 | |
最终黏度Final viscosity (cP) | 半糯型粳稻SJ | 1007 ~ 2619 | 1986.35±336.49 b | 16.94 |
普通粳稻CJ | 1313 ~ 3180 | 2488.31±565.37 a | 22.72 | |
崩解值Breakdown (cP) | 半糯型粳稻SJ | 921 ~ 1667 | 1215.62±212.18 a | 17.45 |
普通粳稻CJ | 478 ~ 960 | 792.5±130.79 b | 16.50 | |
消减值Setback (cP) | 半糯型粳稻SJ | −864 ~ −186 | −477.69±188.43 b | 39.45 |
普通粳稻CJ | −1279 ~ −186 | −541.81±265.91 a | 49.08 | |
回复值Consistence (cP) | 半糯型粳稻SJ | 310 ~ 683 | 560.96±81.94 b | 14.61 |
普通粳稻CJ | 140 ~ 1003 | 780.35±255.16 a | 32.70 |
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV (%) |
---|---|---|---|---|
外观Appearance | 半糯型粳稻SJ | 6.9~9.8 | 8.70±0.75 a | 8.62 |
普通粳稻CJ | 7.4~9.6 | 8.78±0.56 a | 6.42 | |
硬度Hardness | 半糯型粳稻SJ | 3.87~5.53 | 4.61±0.52 b | 11.35 |
普通粳稻CJ | 4.94~6.86 | 5.91±0.52 a | 8.75 | |
黏度Viscosity | 半糯型粳稻SJ | 0.86~1.23 | 1.01±0.10 a | 9.71 |
普通粳稻CJ | 0.62~0.95 | 0.76±0.09 b | 12.12 | |
平衡度Balance degree | 半糯型粳稻SJ | 0.18~0.26 | 0.21±0.02 a | 8.42 |
普通粳稻CJ | 0.12~0.20 | 0.15±0.02 b | 14.08 | |
食味值Taste value | 半糯型粳稻SJ | 76.1~89.4 | 83.59±3.25 a | 3.89 |
普通粳稻CJ | 72.8~82.1 | 78.00±2.49 b | 3.19 |
Table 5. Differences in starch physicochemical characteristics of different types of japonica rice varieties
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV (%) |
---|---|---|---|---|
外观Appearance | 半糯型粳稻SJ | 6.9~9.8 | 8.70±0.75 a | 8.62 |
普通粳稻CJ | 7.4~9.6 | 8.78±0.56 a | 6.42 | |
硬度Hardness | 半糯型粳稻SJ | 3.87~5.53 | 4.61±0.52 b | 11.35 |
普通粳稻CJ | 4.94~6.86 | 5.91±0.52 a | 8.75 | |
黏度Viscosity | 半糯型粳稻SJ | 0.86~1.23 | 1.01±0.10 a | 9.71 |
普通粳稻CJ | 0.62~0.95 | 0.76±0.09 b | 12.12 | |
平衡度Balance degree | 半糯型粳稻SJ | 0.18~0.26 | 0.21±0.02 a | 8.42 |
普通粳稻CJ | 0.12~0.20 | 0.15±0.02 b | 14.08 | |
食味值Taste value | 半糯型粳稻SJ | 76.1~89.4 | 83.59±3.25 a | 3.89 |
普通粳稻CJ | 72.8~82.1 | 78.00±2.49 b | 3.19 |
指标 Index | 相关系数 Correlation coefficient | 指标 Index | 相关系数 Correlation coefficient |
---|---|---|---|
整精米长宽比Length-width ratio of polished rice | −0.366** | 峰值黏度Peak viscosity | −0.132 |
整精米粒长Length of polished rice | −0.208 | 热浆黏度Trough viscosity | −0.049 |
整精米粒宽Width of polished rice | 0.271 | 崩解值Breakdown | 0.190 |
垩白粒率Chalky rice percentage | −0.172 | 最终黏度Final viscosity | −0.072 |
垩白度Chalkiness degree | −0.278* | 消减值Setback | −0.044 |
外观Appearance | 0.797*** | 回复值Consistence | −0.139 |
硬度Hardness | −0.284* | 峰值时间Peak time | 0.118 |
黏度Viscosity | 0.177 | 糊化温度Gelatinization temperature | −0.580*** |
平衡度Balance degree | 0.031 | 直链淀粉含量Amylose content | −0.428** |
蛋白质含量Protein content | −0.045 | 胶稠度Gel consistency | 0.474*** |
Table 6. Correlation coefficient between physical and chemical indexes and taste value
指标 Index | 相关系数 Correlation coefficient | 指标 Index | 相关系数 Correlation coefficient |
---|---|---|---|
整精米长宽比Length-width ratio of polished rice | −0.366** | 峰值黏度Peak viscosity | −0.132 |
整精米粒长Length of polished rice | −0.208 | 热浆黏度Trough viscosity | −0.049 |
整精米粒宽Width of polished rice | 0.271 | 崩解值Breakdown | 0.190 |
垩白粒率Chalky rice percentage | −0.172 | 最终黏度Final viscosity | −0.072 |
垩白度Chalkiness degree | −0.278* | 消减值Setback | −0.044 |
外观Appearance | 0.797*** | 回复值Consistence | −0.139 |
硬度Hardness | −0.284* | 峰值时间Peak time | 0.118 |
黏度Viscosity | 0.177 | 糊化温度Gelatinization temperature | −0.580*** |
平衡度Balance degree | 0.031 | 直链淀粉含量Amylose content | −0.428** |
蛋白质含量Protein content | −0.045 | 胶稠度Gel consistency | 0.474*** |
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