Improvement of the Resistance of Nanjing 9108 to Blast and Sheath Blight by Pyramiding Resistance Gene Pigm and Quantitative Trait Genes qSB-9TQ and qSB-11HJX

doi:10.16819/j.1001-7216.2023.220413

Chinese Journal OF Rice Science ›› 2023, Vol. 37 ›› Issue (2): 125-132.DOI: 10.16819/j.1001-7216.2023.220413

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Improvement of the Resistance of Nanjing 9108 to Blast and Sheath Blight by Pyramiding Resistance Gene Pigm and Quantitative Trait Genes qSB-9^TQ and qSB-11^HJX

WANG Yu¹^,^#, SUN Quanyi¹^,^#, DU Haibo¹, XU Zhiwen¹, WU Keting¹, YIN Li¹, FENG Zhiming¹^,², HU Keming¹^,², CHEN Zongxiang¹^,²^,^*(), ZUO Shimin¹^,²^,³^,^*()

¹Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding / Key Laboratory of Plant Functional Genomics of Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou 225009, China
²Jiangsu Collaborative Innovation Center for Modern Industrial Technology of Grain Crops / Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China
³Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225009

Received:2022-04-24 Revised:2022-09-03 Online:2023-03-10 Published:2023-03-10
Contact: CHEN Zongxiang, ZUO Shimin
About author:First author contact:^#These authors contributed equally to this work

利用抗稻瘟病基因Pigm和抗纹枯病数量性状基因qSB-9^TQ、qSB-11^HJX改良南粳9108的抗性

王雨¹^,^#, 孙全翌¹^,^#, 杜海波¹, 许志文¹, 吴科霆¹, 尹力¹, 冯志明¹^,², 胡珂鸣¹^,², 陈宗祥¹^,²^,^*(), 左示敏¹^,²^,³^,^*()

¹扬州大学农学院/江苏省作物基因组学和分子育种重点实验室/植物功能基因组学教育部重点实验室，江苏扬州 225009
²扬州大学江苏省粮食作物现代产业技术协同创新中心/江苏省作物遗传生理重点实验室, 江苏扬州 225009
³扬州大学教育部农业与农产品安全国际合作联合实验室，江苏扬州 225009

通讯作者: 陈宗祥,左示敏
作者简介:第一联系人：^#共同第一作者
基金资助:
江苏省重点研发计划资助项目(BE2019339);国家自然科学基金资助项目(31872858);扬州市重点研发计划资助项目(YZ2020031);江苏省作物基因组学和分子育种重点实验室开放课题(PL201905);扬州大学研究生国际学术交流专项基金资助项目(YZUIAEF201701008);江苏省研究生科研与实践创新计划项目(KYCX18_2370)

Abstract

Abstract:

【Objective】 Blast and sheath blight are two major diseases in rice, causing huge yield and quality losses. Breeding resistant varieties is the most economical and effective approach to control the two diseases. 【Method】 Using molecular marker assisted selection, we introduced the broad-spectrum blast resistant gene Pigm and quantitative resistance genes qSB-9^TQ, qSB-11^HJX to sheath blight into Nanjing 9108, a japonica rice cultivar with good grain quality. Plant lines with different gene/gene combinations were obtained and the resistance phenotypes were identified as well as the main agronomic and quality traits. 【Result】 The inoculation results showed that Pigm introduction could increase rice resistance to seedling blast and panicle blast significantly. Both qSB-9^TQ and qSB-11^HJX could improve rice sheath blight resistance significantly. Moreover, pyramiding of the two quantitative resistance genes had an additive effect on sheath blight resistance. As for the main agronomic and quality traits, the lines with Pigm showed increased panicle length, number of grains per panicle and the lines with qSB-11^HJX showed significantly increased grain weight. Incorporating these genes exerted no significant effect on other agronomic and quality traits. 【Conclusion】 Pyramiding the three genes could improve blast and sheath blight resistance without apparently negative effect on agronomic and quality traits, and the pyramided rice line can be used as a new germplasm for breeding disease-resistant japonica rice varieties.

Key words: blast, sheath blight, gene pyramiding, japonica rice, resistance improvement

摘要：

【目的】稻瘟病和纹枯病是水稻两大重要病害，严重影响稻米的产量和品质。培育抗病品种是降低其危害最经济有效的措施。【方法】本研究结合分子标记辅助选择技术，将广谱抗稻瘟病基因Pigm和抗纹枯病数量性状基因qSB-9^TQ、qSB-11^HJX导入优良食味粳稻品种南粳9108中，构建不同抗性基因/基因组合的株系，并评价这些株系的稻瘟病和纹枯病抗性，考查其主要农艺性状和品质性状。【结果】导入Pigm能显著提高南粳9108对苗瘟和穗颈瘟的抗性；分别导入qSB-9^TQ、qSB-11^HJX均能提高南粳9108的纹枯病抗性，且两个抗性基因聚合时呈现一定的抗性累加效应。其中，导入Pigm的株系穗长和每穗粒数显著增加，导入qSB-11^HJX的株系千粒重显著增加，其他农艺及品质性状与南粳9108无明显差异。【结论】这些抗性基因导入/聚合能在不降低农艺及品质性状的同时，显著提高纹枯病和稻瘟病的抗性水平，为粳稻抗病育种提供新的种质资源。

关键词: 稻瘟病, 纹枯病, 基因聚合, 粳稻, 抗性改良

WANG Yu, SUN Quanyi, DU Haibo, XU Zhiwen, WU Keting, YIN Li, FENG Zhiming, HU Keming, CHEN Zongxiang, ZUO Shimin. Improvement of the Resistance of Nanjing 9108 to Blast and Sheath Blight by Pyramiding Resistance Gene Pigm and Quantitative Trait Genes qSB-9^TQ and qSB-11^HJX[J]. Chinese Journal OF Rice Science, 2023, 37(2): 125-132.

王雨, 孙全翌, 杜海波, 许志文, 吴科霆, 尹力, 冯志明, 胡珂鸣, 陈宗祥, 左示敏. 利用抗稻瘟病基因Pigm和抗纹枯病数量性状基因qSB-9^TQ、qSB-11^HJX改良南粳9108的抗性[J]. 中国水稻科学, 2023, 37(2): 125-132.

Figures/Tables 7

References 34

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目标基因 Gene	标记 Primer	正向引物 Forward primer (5'-3')	反向引物 Reverse primer (5'-3')
Pigm	M143104	CCTTGTTCCTCCTGCTATC	ATCTCGCTGTTCAGTCTTG
qSB-9^TQ	Y93.5	CTGTTCTTCTCCTGCGTTCT	ATGTCCTCGTGCTTCTGC
	Y90.2	CGGGATTAAATACGAGACAT	TTTCTTAGGTCCCATTCTTC
	Y84.2	AAAGGTTGCGAGGAGATTAGAGT	TAGGGGTTGGTTTCTGGTTGTAG
qSB-11^HJX	RM224	ATCGATCGATCTTCACGAGG	TGCTATAAAAGGCATTCGGG
qSB-11^HJX	ZYJ28.23	AGGGCACAGAGGGAACAAT	ACAGGGTCAGGCAGTCAGG

目标基因 Gene	标记 Primer	正向引物 Forward primer (5'-3')	反向引物 Reverse primer (5'-3')
Pigm	M143104	CCTTGTTCCTCCTGCTATC	ATCTCGCTGTTCAGTCTTG
qSB-9^TQ	Y93.5	CTGTTCTTCTCCTGCGTTCT	ATGTCCTCGTGCTTCTGC
	Y90.2	CGGGATTAAATACGAGACAT	TTTCTTAGGTCCCATTCTTC
	Y84.2	AAAGGTTGCGAGGAGATTAGAGT	TAGGGGTTGGTTTCTGGTTGTAG
qSB-11^HJX	RM224	ATCGATCGATCTTCACGAGG	TGCTATAAAAGGCATTCGGG
qSB-11^HJX	ZYJ28.23	AGGGCACAGAGGGAACAAT	ACAGGGTCAGGCAGTCAGG

编号 No.	基因型 Genotype	菌株数 No. of strains			抗谱 Resistance frequency/%
编号 No.	基因型 Genotype	总数Total	抗Resistant	感Susceptible	抗谱 Resistance frequency/%
W1	Pg⁺9^TQ+11^HJX+	23	23	0	100.0
W2	Pg⁻ 9^TQ+11^HJX+	23	9	14	39.1
W3	Pg⁺9^TQ+11^HJX−	23	22	1	95.6
W4	Pg⁻ 9^TQ+11^HJX−	23	11	12	47.8
W5	Pg⁺9^TQ− 11^HJX+	23	23	0	100.0
W6	Pg⁻ 9^TQ⁻11^HJX⁺	23	10	13	43.5
W7	Pg⁺9^TQ⁻11^HJX⁻	23	23	0	100.0
W8	Pg⁻ 9^TQ− 11^HJX−	23	7	16	30.4

编号 No.	基因型 Genotype	菌株数 No. of strains			抗谱 Resistance frequency/%
编号 No.	基因型 Genotype	总数Total	抗Resistant	感Susceptible	抗谱 Resistance frequency/%
W1	Pg⁺9^TQ+11^HJX+	23	23	0	100.0
W2	Pg⁻ 9^TQ+11^HJX+	23	9	14	39.1
W3	Pg⁺9^TQ+11^HJX−	23	22	1	95.6
W4	Pg⁻ 9^TQ+11^HJX−	23	11	12	47.8
W5	Pg⁺9^TQ− 11^HJX+	23	23	0	100.0
W6	Pg⁻ 9^TQ⁻11^HJX⁺	23	10	13	43.5
W7	Pg⁺9^TQ⁻11^HJX⁻	23	23	0	100.0
W8	Pg⁻ 9^TQ− 11^HJX−	23	7	16	30.4

编号 No.	类型 Genotype	生育期	株高	穗长	一次枝梗数	二次枝梗数	每穗粒数 Grain number	结实率	千粒重
编号 No.	类型 Genotype	Heading date/d	Plant height / cm	Panicle length / cm	No. of primary rachis branches	No. of secondary rachis branches	每穗粒数 Grain number	Seed setting rate/%	1000-grain weight/g
W1	Pg⁺9^TQ⁺11^HJX⁺	82.2±1.0	116.7±2.2^a	21.12±0.06^a	16.4±1.4	45.9±9.4	149.34±3.95^a	88±4	27.57±0.21^a
W2	Pg⁻9^TQ⁺11^HJX⁺	82.7±0.8	108.5±4.5^b	21.28±0.25^a	16.8±1.3	42.1±10.4	151.69±6.42^a	87±3	26.34±0.16^cd
W3	Pg⁺9^TQ⁺11^HJX⁻	81.3±1.5	110.0±3.1^ab	21.45±0.28^a	15.8±0.8	47.8±8.0	150.79±12.15^a	88±4	27.41±0.15^ab
W4	Pg⁻9^TQ⁺11^HJX⁻	81.5±0.5	114.3±2.0^ab	20.39±0.20^b	15.8±1.8	43.9±7.9	134.86±8.07^ab	88±3	27.36±0.13^ab
W5	Pg⁺9^TQ-11^HJX+	81.7±1.0	110.4±2.2^ab	19.86±0.12^b	15.6±0.4	43.1±5.1	135.33±1.29^ab	89±1	26.73±0.26^bc
W6	Pg⁻9^TQ⁻11^HJX⁺	80.7±0.8	108.2±1.0^b	20.33±0.30^b	15.5±0.8	44.9±2.3	135.47±3.16^ab	90±3	27.91±0.52^a
W7	Pg⁺9^TQ⁻11^HJX⁻	82.5±2.2	108.5±3.0^b	21.41±0.39^a	16.1±0.2	49.7±4.2	149.50±1.88^a	88±1	25.92±0.13^d
W8	Pg⁻9^TQ⁻11^HJX⁻	82.8±0.3	109.3±2.6^ab	20.14±0.07^b	16.0±0.2	44.5±0.9	132.06±1.52^b	88±5	26.80±0.08^bc

Improvement of the Resistance of Nanjing 9108 to Blast and Sheath Blight by Pyramiding Resistance Gene Pigm and Quantitative Trait Genes qSB-9^TQ and qSB-11^HJX

利用抗稻瘟病基因Pigm和抗纹枯病数量性状基因qSB-9^TQ、qSB-11^HJX改良南粳9108的抗性

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