Chinese Journal OF Rice Science ›› 2021, Vol. 35 ›› Issue (6): 554-564.DOI: 10.16819/j.1001-7216.2021.200915
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Huan CUI1, Qiaoli GAO1, Lixin LUO1, Jing YANG1, Chun CHEN1, Tao GUO1, Yongzhu LIU1, Yongxiang HUANG2, Hui WANG1, Zhiqiang CHEN1,*(), Wuming XIAO1,*()
Received:
2020-09-23
Revised:
2021-03-04
Online:
2021-11-10
Published:
2021-11-10
Contact:
Zhiqiang CHEN, Wuming XIAO
崔欢1, 高巧丽1, 罗立新1, 杨靖1, 陈淳1, 郭涛1, 刘永柱1, 黄永相2, 王慧1, 陈志强1,*(), 肖武名1,*()
通讯作者:
陈志强,肖武名
基金资助:
Huan CUI, Qiaoli GAO, Lixin LUO, Jing YANG, Chun CHEN, Tao GUO, Yongzhu LIU, Yongxiang HUANG, Hui WANG, Zhiqiang CHEN, Wuming XIAO. Transcriptome Analysis of Hormone Signal Transduction and Glutathione Metabolic Pathway in Rice Seeds at Germination Stage[J]. Chinese Journal OF Rice Science, 2021, 35(6): 554-564.
崔欢, 高巧丽, 罗立新, 杨靖, 陈淳, 郭涛, 刘永柱, 黄永相, 王慧, 陈志强, 肖武名. 水稻萌发期激素信号转导和谷胱甘肽代谢转录分析[J]. 中国水稻科学, 2021, 35(6): 554-564.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2021.200915
序号 No. | 基因号 Gene ID | 正向引物(5′-3′) Forward primer(5′-3′) | 反向引物(3′-5′) Reverse primer(3′-5′) |
---|---|---|---|
1 | Os01g0764800 | TGATCACTCACTACACTACACG | ACACTGACACCGACTGTATAAG |
2 | Os07g0592600 | CCTCTTCCTCTCGCTACTACTA | TCTCAACCCCGAACAAGAAAAA |
3 | Os05g0143800 | ATACTTCGAGTTCATCCCGTTC | CCGACTTTGTACCGGTACAG |
4 | Os11g0528700 | AACATGTGCTACTACGAGTTCA | GTACAACCCTGTGAAGGTGG |
5 | Os01g0370200 | CGAGGCCATCTATCAAGAAGAT | CTTTTGTTACGAGGCACAAGAA |
6 | Os03g0283100 | AAGATTGTCGCGATTGATCTTG | TGATTGTTGTGCTCAAGTGAAG |
7 | Os10g0528300 | CAAGATCTTCGACGAGGAGAAG | CTCATCTTAGCGAACTCGACC |
8 | Os10g0529300 | GACCTCCACAACAAGAGTGAG | AACTTGTCATTGATGTAGGCGG |
9 | Os03g0718100 | GAATGCTAAGCCAAGAGGAG | AATCACAAGTGAGAACCACAG |
Table 1 Sequences of the primers used in qRT-PCR.
序号 No. | 基因号 Gene ID | 正向引物(5′-3′) Forward primer(5′-3′) | 反向引物(3′-5′) Reverse primer(3′-5′) |
---|---|---|---|
1 | Os01g0764800 | TGATCACTCACTACACTACACG | ACACTGACACCGACTGTATAAG |
2 | Os07g0592600 | CCTCTTCCTCTCGCTACTACTA | TCTCAACCCCGAACAAGAAAAA |
3 | Os05g0143800 | ATACTTCGAGTTCATCCCGTTC | CCGACTTTGTACCGGTACAG |
4 | Os11g0528700 | AACATGTGCTACTACGAGTTCA | GTACAACCCTGTGAAGGTGG |
5 | Os01g0370200 | CGAGGCCATCTATCAAGAAGAT | CTTTTGTTACGAGGCACAAGAA |
6 | Os03g0283100 | AAGATTGTCGCGATTGATCTTG | TGATTGTTGTGCTCAAGTGAAG |
7 | Os10g0528300 | CAAGATCTTCGACGAGGAGAAG | CTCATCTTAGCGAACTCGACC |
8 | Os10g0529300 | GACCTCCACAACAAGAGTGAG | AACTTGTCATTGATGTAGGCGG |
9 | Os03g0718100 | GAATGCTAAGCCAAGAGGAG | AATCACAAGTGAGAACCACAG |
Fig. 1. Differentially expressed gene analysis during different germination stages. A, MA map of DEG in early germination; B, MA map of DEG in late germination; C, Venn diagram of DEG in germination stage; D, Venn diagram of up-regulation and down-regulation DEG in germination stage. C0, 0 h after germination; C1, 24 h after germination; C2, 48 h after germination.
Fig. 3. KEGG enrichment plots of DEGs at different germination stages. C0, 0 h after germination; C1, 24 h after germination; C2, 48 h after germination.
Fig. 7. Expression patterns of selected DEG were verified by qRT-PCR. C0, 0 h after germination; C1, 24 h after germination; C2, 48 h after germination. FPKM, Fragments per kilobase per million.
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