Chinese Journal OF Rice Science ›› 2021, Vol. 35 ›› Issue (4): 352-358.DOI: 10.16819/j.1001-7216.2021.200908
• Orginal Article • Previous Articles Next Articles
Chen WANG1, Beifang WANG1, Yingxin ZHANG1, Yongrun CAO1, Yue ZHANG1, Min JIANG1, Kangji BIAN2, Xiaohui ZHANG1,2,*(), Qunen LIU1,*()
Received:
2020-09-11
Revised:
2020-11-13
Online:
2021-07-10
Published:
2021-07-10
Contact:
Xiaohui ZHANG, Qunen LIU
王晨1, 王备芳1, 张迎信1, 曹永润1, 张越1, 江敏1, 边康吉2, 张小惠1,2,*(), 刘群恩1,*()
通讯作者:
张小惠,刘群恩
基金资助:
Chen WANG, Beifang WANG, Yingxin ZHANG, Yongrun CAO, Yue ZHANG, Min JIANG, Kangji BIAN, Xiaohui ZHANG, Qunen LIU. Identification and Gene Mapping of a Lesion Mimic Mutant lm8015-2 in Rice[J]. Chinese Journal OF Rice Science, 2021, 35(4): 352-358.
王晨, 王备芳, 张迎信, 曹永润, 张越, 江敏, 边康吉, 张小惠, 刘群恩. 水稻类病斑突变体lm8015-2的鉴定与基因的精细定位[J]. 中国水稻科学, 2021, 35(4): 352-358.
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2021.200908
标记 Marker | 正向引物 Forward primer (5′-3′) | 反向引物 Reverse primer (5′-3′) |
---|---|---|
W32-2 | CGGGAAGCTGAACCGGC | GTACCGGAATTTCCACACCTT |
W32-14 | GTGTGCTTGCTGCGGATATG | GCTAACACACCCAACGAATGA |
W32-44 | TGGCTTTGTAGATGGCCGATT | ATCCAAAGTGTTGTGGGCCT |
W32-85 | CGGAAATCCCCTCACGCAC | CTGTTGGAGATGCTCTAACCCT |
C32-8 | CCAAGAGGTCTGGAGATTGC | TGGAGCTCCTGGAACAACAT |
PR1B | CATTGCTTTGGCCATGGTAG | GAACCCCAGAAGAGGTTCTC |
PR5 | CTTCTGCCCATAATGCATCATC | TGATTATCGATCAAGGTGTCGT |
PR10 | GGTGTGGGAAGCACATACAA | GTCTCCGTCGAGTGTGACTTG |
AOS2 | AAGCTGCTGCAATACGTGTACTGG | CGACGAGCAACAGCCTTCCG |
UBQ | TGGTCAGTAATCAGCCAGTTTGG | GCACCACAAATACTTGACGAACAG |
Table 1 Primers used for mapping and qRT-PCR.
标记 Marker | 正向引物 Forward primer (5′-3′) | 反向引物 Reverse primer (5′-3′) |
---|---|---|
W32-2 | CGGGAAGCTGAACCGGC | GTACCGGAATTTCCACACCTT |
W32-14 | GTGTGCTTGCTGCGGATATG | GCTAACACACCCAACGAATGA |
W32-44 | TGGCTTTGTAGATGGCCGATT | ATCCAAAGTGTTGTGGGCCT |
W32-85 | CGGAAATCCCCTCACGCAC | CTGTTGGAGATGCTCTAACCCT |
C32-8 | CCAAGAGGTCTGGAGATTGC | TGGAGCTCCTGGAACAACAT |
PR1B | CATTGCTTTGGCCATGGTAG | GAACCCCAGAAGAGGTTCTC |
PR5 | CTTCTGCCCATAATGCATCATC | TGATTATCGATCAAGGTGTCGT |
PR10 | GGTGTGGGAAGCACATACAA | GTCTCCGTCGAGTGTGACTTG |
AOS2 | AAGCTGCTGCAATACGTGTACTGG | CGACGAGCAACAGCCTTCCG |
UBQ | TGGTCAGTAATCAGCCAGTTTGG | GCACCACAAATACTTGACGAACAG |
Fig. 1. Phenotype and pigment contents of wild type ZH8015 and the mutant lm8015-2. Bar= 2 cm. A, Three weeks after seeding; B, Initial tillering stage; C, Full tillering stage; D, Leaves before shading; E, Leaves after seven days of shading; F, DAB staining; G, EB straining; J, Pigment contents. ZH8015, Zhonghui 8015. **Significant at P<0.01(t-test).
Fig. 2. Physiological indices of the wild type ZH8015 and its mutant lm8015-2 at the tillering stage. **Significant at P<0.01(t-test); ZH8015, Zhonghui 8015.
组合Cross | F1 | F2 | 比例 Ratio | χ2 (3:1) | |
---|---|---|---|---|---|
无斑点株数 No. of normal plants | F2有斑点株数 No. of spotted plants | ||||
02428/lm8015-2 | 无斑点 Normal | 3 796 | 1 217 | 3.11∶1 | 0.40 |
Table 2 Genetic analysis of lm8015-2.
组合Cross | F1 | F2 | 比例 Ratio | χ2 (3:1) | |
---|---|---|---|---|---|
无斑点株数 No. of normal plants | F2有斑点株数 No. of spotted plants | ||||
02428/lm8015-2 | 无斑点 Normal | 3 796 | 1 217 | 3.11∶1 | 0.40 |
[1] | Roohollah S D, Angelica L, Aliakbari M, Hadi P A.Plausible association between drought stress tolerance of barley (Hordeum vulgare L.) and programmed cell death via MC1 and TSN1 genes[J]. Physiologia Plantarum, 2020, 170(1): 46-59. |
[2] | Lin C H, Pan Y C, Ye N H, Shih Y T, Liu F W, Chen C Y.LsGRP1, a class II glycine-rich protein of Lilium, confers plant resistance via mediating innate immune activation and inducing fungal programmed cell death[J]. Molecular Plant Pathology, 2020, 21(9): 1149-1166. |
[3] | Niazian M, Shariatpanahi M E.In vitro-based doubled haploid production: Recent improvements[J]. Euphytica, 2020, 216(5): 1-21. |
[4] | 张宏根, 王茂宇, 张丽佳, 胡雅, 马佳琦, 张翼帆, 汤述翥, 梁国华, 顾铭洪. 水稻类病斑突变体wy3的鉴定和基因定位[J]. 中国水稻科学, 2016, 30(3): 239-246. |
Zhang H G, Wang M Y, Zhang L J, Hu Y, Ma J Q, Zhang Y F, Tang S Z, Liang G H, Gu M H.Characterization and gene mapping of lesion mimic mutant wy3 in rice[J]. Chinese Journal of Rice Science, 2016, 30(3): 239-246. (in Chinese with English abstract) | |
[5] | Shirasu K, Schulze-Lefert P.Regulators of cell death in disease resistance[J]. Plant Molecular Biology, 2000, 44(3): 371-385. |
[6] | Yuan Y, Zhong S, Li Q, Zhu Z, Lou Y, Wang L, Wang J, Wang M, Yang D, Li Q, He Z.Functional analysis of rice NPR1-like genes reveals that OsNPR1/NH1 is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility[J]. Plant Biotechnology Journal, 2010, 5(2): 313-324. |
[7] | Yamanouchi U, Yano M, Lin H X, Ashikari M, Yamada K.A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein[J]. Proceedings of the National Academy of Sciences, 2002, 99(11): 7530-7535. |
[8] | You Q Y, Zhai K, Yang D L, Yang W B, Wu J N, Liu J Z, Pan W B, Wang J J, Zhu X D, Jian Y K, Liu J Y, Zhang Y Y, Li Q, Lou Y G, Xie Q, He Z H.An E3 ubiquitin ligase-BAG protein module controls plant innate immunity and broad-spectrum disease resistance[J]. Cell Host&Microbe, 2016, 20(6): 758-769. |
[9] | Fekih R, Tamiru M, Kanzaki H, Abe A, Yoshida K, Kanzaki E, Saitoh H, Takagi H, Natsume S, Undan J R, Undan J, Terauchi R.The rice (Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response[J]. Molecular Genetics & Genomics, 2015, 290(2): 611-622. |
[10] | Arnon D I.Copper enzyme in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris[J]. Plant Physiology, 1949, 24(1): 1-15. |
[11] | Wellburn A R.The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution[J]. Journal of Plant Physiology, 1994, 144(3): 307-313. |
[12] | Thordal-Christensen H, Zhang Z G, Wei Y D, Collinge D B.Subcellular localization of H2O2 in plants: H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction[J]. Plant Journal, 1997, 11(6): 1187-1194. |
[13] | Li R, L S Y, Xu Z X. Studies on the programmed cell death in rice during starchy endosperm development[J]. Agricultural Sciences in China, 2004, 3(9): 663-670. |
[14] | Rogers S O, Bendich A J.Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues[J]. Plant Molecular Biology, 1985, 5(2): 69-76. |
[15] | 孙德权, 郭启高, 胡玉林, 谢江辉. 改良Trizol法提取香蕉叶片总RNA[J]. 广东农业科学, 2009(5): 162-164. |
Sun D Q, Guo Q G, Hu Y L, Xie J H.Extraction of total RNA from banana with improved Trizol method[J]. Guangdong Agricultural Sciences, 2009(5): 162-164. (in Chinese) | |
[16] | Cao Y, Yan Y, Zhang F, Wang H D, Gu M, Wu X N, Sun S B, Xu G H.Fine characterization of OsPHO2 knockout mutants reveals its key role in Pi utilization in rice[J]. Journal of Plant Physiology, 2014, 171(3-4): 340-348. |
[17] | Pickart C M, Eddins M J.Ubiquitin: Structures, functions, mechanisms[J]. Biochimica et Biophysica Acta, 2004, 1695(1-3): 55-72. |
[18] | Zeng L R, Qu S H, Bordeos A, Yang C W, Baraoidan M, Yan H Y, Xie Q, Nahm B H, Leung H, Wang G L.Spotted leaf11, a negative regulator of plant cell death and defense, encodes a U-box/armadillo repeat protein endowed with E3 ubiquitin ligase activity[J]. The Plant Cell, 2004, 16(10): 2795-2808. |
[19] | Ma J, Wang Y F, Ma X D, Meng L Z, Jing R N, Wang F, Wang S, Cheng Z J, Zhang X, Jiang L, Wang J L, Wang J, Zhao Z C, Zhao Z C, Guo X P, Lin Q B, Wu F Q, Zhu S S, Wu C Y, Ren Y L, Lei C, Zhai H Q, Wan J M.Disruption of gene SPL35, encoding a novel CUE domain-containing protein, leads to cell death and enhanced disease response in rice[J]. Plant Biotechnology Journal, 2019, 17(8): 1679-1693. |
[20] | Hu B, Zhu C, Li F, Tang J Y, Wang Y Q, Lin A H, Liu L C, Che R H, Chu C C.LEAF TIP NECROSIS1 plays a pivotal role in the regulation of multiple phosphate starvation responses in rice[J]. Plant Physiology, 2011, 156(3): 1101-1115. |
[21] | Hu B, Wang W, Deng K, Li H, Zhang Z H, Zhang L H, Chu C C.MicroRNA399 is involved in multiple nutrient starvation responses in rice[J]. Frontiers in Plant Science, 2015, 188(6): 1-10. |
[22] | E Z G, Zhang Y P, Li T T, Wang L, Zhao H M. Characterization of the ubiquitin-conjugating enzyme gene family in rice and evaluation of expression profiles under abiotic stresses and hormone treatments[J]. PloS One, 2015, 10(4): e0122621. |
[23] | 乐美旺, 陈实, 潘庆华, 曾任森, 刘迎湖, 骆世明. 水稻和稻瘟病菌互作中的信号传导及防御反应基因诱导表达的研究[J]. 植物病理学报, 2007, 37(1): 42-49. |
Le M W, Chen S, Pan Q H, Zeng R S, Liu Y H, Tuo S M.Induced expression of signaling pathway and defense-related genes in interaction between rice and Magnaporthe grisea[J]. Acta Phytopathologica Sinica, 2007, 37(1): 42-49. (in Chinese with English abstract) |
[1] | REN Zhiqi, XUE Kexin, DONG Zheng, LI Xiaoxiang, LI Yongzhao, GUO Yujing, LIU Wenqiang, GUO Liang, SHENG Xinnian, LIU Zhixi, PAN Xiaowu. Identification and Gene Mapping of Outcurved Leaf Mutant ocl1 in Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 337-346. |
[2] | XIAO Lequan, LI Lei, DAI Weimin, QIANG Sheng, SONG Xiaoling. Seedling Growth Characteristics of Hybrids Between Transgenic Rice with cry2A*/bar Genes and Weedy Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 347-358. |
[3] | LI Gang, GAO Qingsong, LI Wei, ZHANG Wenxia, WANG Jian, CHEN Baoshan, WANG Di, GAO Hao, XU Weijun, CHEN Hongqi, JI Jianhui. Directed Knockout of SD1 Gene Improves Lodging Resistance and Blast Resistance of Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 359-367. |
[4] | WANG Shengyong, CHEN Yuhang, CHEN Huili, HUANG Yujie, ZHANG Xiaotian, DING Shuangcheng, WANG Hongwei. Effects of High Temperature on Phenylpropane Metabolism and Downstream Branch Metabolic Pathways in Rice Meiosis [J]. Chinese Journal OF Rice Science, 2023, 37(4): 368-378. |
[5] | HUANG Yaru, XU Peng, WANG Lele, HE Yizhe, WANG Hui, KE Jian, HE Haibing, WU Liquan, YOU Cuicui. Effects of Exogenous Trehalose on Grain Filling Characteristics and Yield Formation of japonica Rice Cultivar W1844 [J]. Chinese Journal OF Rice Science, 2023, 37(4): 379-391. |
[6] | DONG Liqiang, YANG Tiexin, LI Rui, SHANG Wenqi, MA Liang, LI Yuedong, SUI Guomin. Effect of Plant-row Spacing on Rice Yield and Root Morphological and Physiological Characteristics in Super High Yield Field [J]. Chinese Journal OF Rice Science, 2023, 37(4): 392-404. |
[7] | GAO Qianqing, REN Xiaojian, ZHAI Zhongbing, ZHENG Pubing, WU Yuanfen, CUI Kehui. Effects of Panicle and Bud-promoting Nitrogen Fertilizer Application on Growth of Regenerated Bud and Grain Yield of Ratoon Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 405-414. |
[8] | HUANG Jinwen, LI Rikun, CHEN Zhicheng, ZHANG Bianhong, LEI Han, PAN Ruixin, YANG Mingyu, PAN Meiqing, TANG Lina. Effects of Straw Returning Techniques on Soil Nutrients, Organic Carbon and Microbial Diversity in Tobacco-rice Rotation System [J]. Chinese Journal OF Rice Science, 2023, 37(4): 415-426. |
[9] | HAN Cong, HE Yuchang, WU Lijuan, JIA Lili, WANG Lei, E Zhiguo. Research Progress in the Function of Basic Leucine Zipper (bZIP) Protein Family in Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 436-448. |
[10] | SHEN Yumin, CHEN Mingliang, XIONG Huanjin, XIONG Wentao, WU Xiaoyan, XIAO Yeqing. Phenotypic Analysis and Fine Mapping of blg1(beak like grain 1), a Rice Mutant with Abnormal Palea and Lemma Development [J]. Chinese Journal OF Rice Science, 2023, 37(3): 225-232. |
[11] | DUAN Min, XIE Liujie, GAO Xiuying, TANG Haijuan, HUANG Shanjun, PAN Xiaobiao. Creation of Thermo-sensitive Genic Male Sterile Rice Lines with Wide Compatibility Based on CRISPR/Cas9 Technology [J]. Chinese Journal OF Rice Science, 2023, 37(3): 233-243. |
[12] | WANG Wenting, MA Jiaying, LI Guangyan, FU Weimeng, LI Hubo, LIN Jie, CHEN Tingting, FENG Baohua, TAO Longxing, FU Guanfu, QIN Yebo. Effect of Different Fertilizer Application Rates on Rice Yield and Quality Formation and Its Relationship with Energy Metabolism at High Temperature [J]. Chinese Journal OF Rice Science, 2023, 37(3): 253-264. |
[13] | LIU Aihua, LI Xiaokun. Meta-analysis of Relationship Between Fertilizer Application and Rice Quality [J]. Chinese Journal OF Rice Science, 2023, 37(3): 276-284. |
[14] | YANG Xiaolong, WANG Biao, WANG Benfu, ZHANG Zhisheng, ZHANG Zuolin, YANG Lantian, CHENG Jianping, LI Yang. Effects of Different Water Management on Yield and Rice Quality of Dry-seeded Rice [J]. Chinese Journal OF Rice Science, 2023, 37(3): 285-294. |
[15] | WEI Xiaodong, SONG Xuemei, ZHAO Ling, ZHAO Qingyong, CHEN Tao, LU Kai, ZHU Zhen, HUANG Shengdong, WANG Cailin, ZHANG Yadong. Effects of Silicon and Zinc Fertilizer and Their Application Ways on Yield and Grain Quality of Rice Variety Nanjing 46 [J]. Chinese Journal OF Rice Science, 2023, 37(3): 295-306. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||