Chinese Journal OF Rice Science ›› 2017, Vol. 31 ›› Issue (5): 457-465.DOI: 10.16819/j.1001-7216.2017.7031 457
• Orginal Article • Previous Articles Next Articles
Huimin FENG*(), Hong LU, Hanqing WANG, Xinyue LI
Received:
2017-03-15
Revised:
2017-04-06
Online:
2017-10-10
Published:
2017-09-10
Contact:
Huimin FENG
通讯作者:
冯慧敏
基金资助:
CLC Number:
Huimin FENG, Hong LU, Hanqing WANG, Xinyue LI. Function Analyses of Rice Nitrate Transporter Gene OsNPF7.9 in Nitrogen Accumulation and Transport[J]. Chinese Journal OF Rice Science, 2017, 31(5): 457-465.
冯慧敏, 陆宏, 王汉卿, 李昕玥. 水稻硝酸盐转运蛋白基因OsNPF7.9在氮素积累和转运中的 功能研究[J]. 中国水稻科学, 2017, 31(5): 457-465.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2017.7031 457
基因名称 Gene | 引物序列 Primer sequence(5′-3′) | 退火温度 Annealing temperature/℃ | 循环数 No. of cycles | 产物大小 Product size/ bp | ||
---|---|---|---|---|---|---|
OsActin | F:GGAACTGGTATGGTCAAGGC R: AGTCTCATGGATAACCGCAG | 55 | 32 | 792 | ||
OsNPF7.9 | F: GCAACGACGGAGACATGAAG R: GCGAGGCCAAGGACAAAGAT | 58 | 28 | 500 |
Table 1 Primers for semi-RT PCR in the study.
基因名称 Gene | 引物序列 Primer sequence(5′-3′) | 退火温度 Annealing temperature/℃ | 循环数 No. of cycles | 产物大小 Product size/ bp | ||
---|---|---|---|---|---|---|
OsActin | F:GGAACTGGTATGGTCAAGGC R: AGTCTCATGGATAACCGCAG | 55 | 32 | 792 | ||
OsNPF7.9 | F: GCAACGACGGAGACATGAAG R: GCGAGGCCAAGGACAAAGAT | 58 | 28 | 500 |
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