Genetic Analysis and Gene Mapping of White Stripe Leaf Mutant st11 in Rice

doi:10.3969/j.issn.1001-7216.2015.01.002

Abstract

Abstract:

A white stripe leaf mutant st11 was obtained from tissue-cultured rice Kitaake (Oryza sativa spp japonica). This mutant was characterized by white stripe leaves from tillering stage to mature stage. Compared with the wild type Kitaake, the mutant showed no obvious changes in agricultural traits, such as tiller number, plant height, seed-setting rate, and grain weight. Genetic analyses showed that the white stripe leaf phenotype in the mutant st11 was controlled by a single recessive gene. Two F₂ segregating populations derived from the crosses, st11×Jodan and st11×02428, respectively, were used for mapping the gene st11. Bulked segregant analysis suggested that the gene st11 was located close to the markers, RM151 and RM10080, on chromosome 1. Using more molecular markers, we finally delimited the gene st11 to a region of about 270 kb between the Indel markers, I10 and I26, on the telomere of the short arm of chromosome 1.

Key words: rice, white stripe leaf mutant, molecular marker, gene mapping

摘要：

白条纹叶突变体 st11是从粳稻品种Kitaake组培过程中获得的。该突变体在分蘖前叶色表现为正常,从分蘖期开始新生叶表现为白条纹直至成熟期。与野生型相比,该突变体的分蘖、株高、结实率和千粒重等农艺性状没有发生明显变化。遗传分析表明该突变体白条纹叶性状受一对隐性核基因控制。利用该突变体分别与水稻02428、Jodan杂交构建了两个F₂群体用于基因定位。通过集群分离分析(bulked segregant analysis)发现该基因位于第1染色体端粒附近,并与分子标记RM151和RM10080连锁。进一步利用更多分子标记分析F₂群体,我们将该基因定位于I10和I26两个标记之间大约270kb的区间内。

关键词: 水稻, 白条纹叶突变体, 分子标记, 基因定位

CLC Number:

Q343.5
S754

Qin-shu CHENG, Bang-quan YE, Can YUAN, Wei-tao LI, Jun-jie YIN, Jing WANG, Min HE, Ji-chun WANG, Yu-ping WANG, Shi-gui LI, Xue-wei CHEN. Genetic Analysis and Gene Mapping of White Stripe Leaf Mutant st11 in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(1): 14-21.

成钦淑, 叶邦全, 袁灿, 李伟滔, 尹俊杰, 王静, 贺闽, 汪吉春, 王玉平, 李仕贵, 陈学伟. 水稻白条纹叶突变体 st11的遗传分析与基因定位[J]. 中国水稻科学, 2015, 29(1): 14-21.

Figures/Tables 9

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分子标记 Marker	正向引物 Forward primer	反向引物 Reverse primer	物理距离^a Distance/Mb^a
I26	ATTCAGAAGGTGATGCTCCG	GATATGTCGCCAGAGACCCT	0.188
I10	TGCAACGACGGGCATTTTG	GAGCTTCTTGATGTAGGCCG	0.455
I14	GGGGCTCAACATGGACCAAA	GGCATGTTGAAGCTGAGCAC	0.689
RM10048	CAAGCAGTGATCATACAGCCTTCC	GCCATGGCTGAGAACAGAGAGC	0.744
RM10076	CTAGCAGCTGTCTGCGACACACG	CCGAGGTGTTATGCCAATCTCTATGG	1.351

分子标记 Marker	正向引物 Forward primer	反向引物 Reverse primer	物理距离^a Distance/Mb^a
I26	ATTCAGAAGGTGATGCTCCG	GATATGTCGCCAGAGACCCT	0.188
I10	TGCAACGACGGGCATTTTG	GAGCTTCTTGATGTAGGCCG	0.455
I14	GGGGCTCAACATGGACCAAA	GGCATGTTGAAGCTGAGCAC	0.689
RM10048	CAAGCAGTGATCATACAGCCTTCC	GCCATGGCTGAGAACAGAGAGC	0.744
RM10076	CTAGCAGCTGTCTGCGACACACG	CCGAGGTGTTATGCCAATCTCTATGG	1.351

组合 Cross	F₁	F₂			X²_(3:1)
组合 Cross	F₁	总株数 Total no. of plants	正常植株 No. of normal plants	突变体植株 No. of white strip plants	X²_(3:1)
st11/Jodan	正常绿叶 Normal green leaf	1035	791	244	0.589
st11/02428	正常绿叶 Normal green leaf	599	453	146	0.054
st11/C418	正常绿叶 Normal green leaf	448	343	105	0.502
C418/st11	正常绿叶 Normal green leaf	421	324	97	0.863

组合 Cross	F₁	F₂			X²_(3:1)
组合 Cross	F₁	总株数 Total no. of plants	正常植株 No. of normal plants	突变体植株 No. of white strip plants	X²_(3:1)
st11/Jodan	正常绿叶 Normal green leaf	1035	791	244	0.589
st11/02428	正常绿叶 Normal green leaf	599	453	146	0.054
st11/C418	正常绿叶 Normal green leaf	448	343	105	0.502
C418/st11	正常绿叶 Normal green leaf	421	324	97	0.863

交换单株号 No. of recombinant plants	标记 Marker
交换单株号 No. of recombinant plants	I26	I10	I14	RM10048	RM10076
46	1	0	0	0	0
51	1	0	0	0	0
84	1	0	0	0	0
24	0	0	1	1	1
81	0	0	1	1	1
93	0	0	1	1	1
23	0	0	0	0	2
41	0	0	0	0	1
44	0	0	0	0	1
60	0	0	0	0	1
74	0	0	0	0	1
81	0	0	0	0	1
84	0	0	0	0	1
107	0	0	0	0	1
110	0	0	0	0	1
112	0	0	0	0	1
115	0	0	0	0	1
165	0	0	0	0	1