Chinese Journal of Rice Science

• 研究报告 • Previous Articles     Next Articles

Development of Molecular Markers for Sbe Gene in Association with Starch Characteristic in Rice

LI Gang1,2,#, DENG Qiming1,2,#, WAN Yingxiu3, LI Shuang cheng1,2,XIAO Yong1,2, ZHOU Peng1,2, WANG Shiquan1,2, LI Ping1,2,*   

  1. 1Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; 2Key Laboratory of Southwest Crop Genetic Resource and Improvement, Ministry of Education, Sichuan Agricultural University, Ya′an 625014, China; 3Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China; # These authors contributed equally to this paper; *Corresponding author, E-mail: liping@cngk.com
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-10 Published:2008-11-10

稻米淀粉理化特性相关的水稻淀粉分支酶基因标记开发

李刚1,2,#,邓其明1,2,#,万映秀3,李双成1,2,肖勇1,2,周鹏1,2,王世全1,2 ,李 平1,2,*   

  1. 1四川农业大学 水稻研究所, 四川 温江 611130; 2四川农业大学 西南作物基因资源与遗传改良教育部重点实验室, 四川 雅安 625014; 3安徽省农业科学院 作物研究所, 安徽 合肥 230031; # 共同第一作者; *通讯联系人, E-mail: liping@cngk.com

Abstract: Based upon the genomic sequence difference between the indica rice variety 9311 and the japonica rice variety Nipponbare, a total of nine molecular markers for starch branching enzyme gene(Sbe) were successfully developed. All the markers were utilized for genotypic screening and further analysis of the correlation between genetic polymorphisms of Sbe1 and Sbe3 genes and the rice starch characteristics. The genetic polymorphism of the markers had no influence on starch physiochemical traits, that is these allelomorphism variation sites of Sbe gene had no influence on the starch traits of nonglutinous rice. There were six markers which could be used to accurately identify indica rice varieties with a reliability over 80%. Meaning of marker development and molecular marker assisted selection were also discussed.

Key words: rice, starch branching enzyme gene, molecular marker, starch viscosity, variety identification

摘要: 基于籼稻9311和粳稻日本晴基因组间的序列差异,成功发展了水稻淀粉分支酶基因(Sbe)9个分子标记。利用这些标记对102份非糯材料进行了基因型检测,并分析了Sbe1、Sbe3基因标记基因型多态性对稻米淀粉理化特性的影响。Sbe1、Sbe3基因标记多态性位点对揭示非糯材料间稻米淀粉理化特性的差异不显著,说明淀粉分支酶基因这些等位性变异位点对非糯稻米的蒸煮食味品质影响不显著;在鉴定籼稻品种时,有6个标记的鉴定准确率达80%以上。还对标记开发的意义和分子标记辅助选择育种进行了探讨。

关键词: 水稻, 淀粉分支酶基因, 分子标记, 淀粉黏滞性