Abstract: Semi-quantitative RT-PCR was performed to examine the expression profile of an aquaporin gene,OsAQP gene,in rice leaves at different time after seed germination. By digoxigenin tagging in situ hybridization techniques,the transcripts of OsAQP gene were detected in rice leaf tissues. The results showed that the expression of OsAQP presented a descendent tendency in rice leaves within 15 days after germination. The transcripts in leaves were mainly in guard cells,weakly in mesophyll cells and seldomly in epidermal cells,which suggested that OsAQP may be involved in the functions of stoma by coupling the rapid transportation of water in guard cells.

Key words: rice, aquaporin gene, reverse transcription polymerase chain reaction, in situ hybridization, gene expression

摘要： 利用半定量RTPCR技术，检测了水稻水通道蛋白基因OsAQP在水稻不同生长时期叶片中的表达情况。并利用地高辛标记原位杂交技术，检测了OsAQP在叶组织中的定位。OsAQP在叶片中的表达水平在水稻种子发芽后15 d内呈现下降的趋势。该基因的转录本在叶片保卫细胞中的信号最强，叶肉细胞中信号较弱，而在表皮细胞中没有检测到信号，提示该基因可能通过介导保卫细胞水分的快速运输而与气孔功能相关。

关键词: 水稻, 水通道蛋白基因, 反转录聚合酶链式反应