Chinese Journal of Rice Science

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Total Content and Polymorphism of Seed Storage Proteins in Rice

JIANG Dong-hua , YANG Bao-feng , YE Yan , LI Jie , HUANG Da-nian , WEI Xing-hua   

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-11-10 Published:2007-11-10
  • Contact: WEI Xing-hua

水稻种子贮藏蛋白总含量分布和多态性分析

蒋冬花1;杨宝峰1;叶砚1;李杰1;黄大年2;魏兴华2   

  1. 1浙江师范大学 化学与生命科学学院, 浙江 金华321004; Email:jdh@zjnu.cn; 2中国水稻研究所 水稻生物学国家重点实验室, 浙江 杭州 310006)

Abstract: Rice seed storage protein contents in 110 rice varieties or lines were determined by using the staining method with Comassie brilliant blue G-250.There were obvious diversities of seed storage protein contents among the varieties or lines tested.The contents of rice seed storage proteins ranged from 34.29 to 95.08 mg/g,but 66.3% varieties or lines were in the range of 40 to 60 mg/g.The rice varieties or lines with more than 80 mg/g of rice seed storage protein contents were rare,only accounting for 2.7% of the total tested materials.The result of SDS-PAGE for seed storage proteins also displayed obvious polymorphisms among the rice varieties or lines.It could provide useful information for seed storage protein analysis and for rice breeding.

Key words: rice, storage protein, electrophresis, polymorphism

摘要: 用考马斯亮蓝G250染色法测定了来自世界各地的110个水稻品种或品系种子贮藏蛋白的含量,发现不同水稻品种或品系种子贮藏蛋白含量存在一定的多样性。110个水稻材料贮藏蛋白含量分布在34.29 ~95.08 mg/g,含量最高的为来自国际水稻研究所的品系C105A51;大部分水稻材料的种子贮藏蛋白含量在40 ~60 mg/g,有72个,占所测水稻材料总数的655%;总蛋白质含量超过80 mg/g的水稻材料很少,只有3个,占2.7 %。SDSPAGE蛋白电泳图谱表明水稻贮藏蛋白种类和含量存在较高的多态性。

关键词: 水稻, 贮藏蛋白, 电泳, 多态性