Abstract: A rice etiolation mutant 824ys featured with chlorophyll deficiency was identified from a normal green rice variety 824B.It showed whole green-yellow plant from the seedling stage,reduced number of tillers and longer growth duration.The contents of chlorophyll,chlorophyll a,chlorophyll b and net photosynthetic rate of the mutant obviously decreased,as well as the number of spikelets per panicle,seed setting rate and 1000-grain weight compared with its wild-type parent.Genetic analysis of F1 and F2 generations of 824ys crossed with three normal green varieties showed that the chlorophyll-deficit mutant character was controlled by one pair of recessive nuclear genes.Genetic mapping of the mutant gene was conducted by using microsatellite markers and F2 mapping population of 495R/824ys,and the mutant gene of 824ys was mapped on the short arm of rice chromosome 3.The genetic distances from the target gene to the markers RM218,RM282 and RM6959 were 25.6,5.2 and 21.8 cM,respectively.It was considered to be a new chlorophyll-deficit mutant gene and tentatively named as chl11(t).

Key words: rice, chlorophyll-deficit mutant, genetic analysis, gene mapping, microsatellite marker

摘要： 从正常绿色水稻品种824B中发现1个黄化突变体824ys。该突变体具有叶绿素缺失突变特性，表现为植株黄绿色，分蘖数减少，生育期延长，总叶绿素、叶绿素a、叶绿素b的含量以及净光合速率比野生型亲本824B明显下降，每穗着粒数、结实率、千粒重等降低。对824ys与3个正常绿色品种杂交F1、F2的遗传分析表明，控制824ys的叶绿素缺失突变性状为1对隐性核基因。以495R/824ys F2作为定位群体，应用微卫星标记将824ys的叶绿素缺失突变基因定位于水稻第3染色体短臂，与RM218、RM282和RM6959等标记之间的遗传距离分别为25.6、 5.2和21.8 cM。认为该基因为一个新的水稻叶绿素缺失突变基因，暂命名为chl11(t)。

关键词: 水稻, 叶绿素缺失突变体, 遗传分析, 基因定位, 微卫星标记