中国水稻科学

中国水稻科学

• 综述与专论 •    下一篇

水稻功能基因的电子克隆策略

黄 骥 1; 张红生 1,*;曹雅君 1; 钱晓茵 2; 杨金水 2   

  1. 1南京农业大学 作物遗传与种质创新国家重点实验室, 江苏 南京 210095； 2复旦大学 遗传学研究所， 上海 200433； * 通讯联系人，E-mail:hszhang@mail.njau.edu.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-10-10 发布日期:2002-10-10

Strategy of in Silico Cloning of Functional Genes in Rice (Oryza sativa)

HUANG Ji 1; ZHANG Hong-sheng 1; CAO Ya-jun 1; QIAN Xiao-yin 2; YANG Jin-shui 2   

  1. （1National Key Laboratory of Crop Genetics and Germplasm Enhancement; Nanjing Agricultural University; Nanjing 210095; China; 2Institute of Genetics; Fudan University, Shanghai 200433, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-10-10 Published:2002-10-10
  • Contact: ZHANG Hong-sheng

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被引次数

105

摘要： 电子克隆是随着基因组计划和EST计划实施发展起来的，利用生物信息学手段进行基因克隆的新方法。已经公布的水稻基因组序列框架图，使得水稻的电子克隆路线得以实施。介绍了水稻电子克隆的基本原理、应用实例、相关的生物信息资源、存在问题和展望。

关键词: 水稻, 电子克隆, 生物信息学

Abstract:

In silico cloning was a new way of gene cloning by bioinfomatics, which developed with genome enforcement and EST project. Since the rice draft sequence of genome was published in public, it was possible to isolate the functional genes of rice by in silico cloning. The principle, application, related resource of bioinformatics, problems and the prospect of in silico cloning of functional genes in rice were reviewed.

Key words: rice, in silico cloning, bioinformatics, functional gene