中国水稻科学

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水稻中花11辐射突变体的分离与鉴定

朱旭东1; 陈红旗1; 罗达2; 张建军3; 方红民1; 闵绍楷1   

  1. 1中国水稻研究所 农业部水稻生物学重点实验室, 浙江 杭州 310006; 2中国科学院 上海植物生理生态研究所,上海 200032; 3上海市农业科学院 作物育种栽培研究所,上海 201106
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-07-10 发布日期:2003-07-10

Screening and Characterization of Mutants Induced from Zhonghua 11 (Oryza sativa L. subsp. japonica) by Irradiation

ZHU Xu-dong 1; CHEN Hong-qi 1; LUO Da 2; ZHANG Jian-jun 3; FANG Hong-min 1; MIN Shao-kai 1   

  1. 1 Key Laboratory for Rice Biology; Ministry of Agriculture; China National Rice Research Institute; Hangzhou 310006; China; 2 Institute of Plant Physiology and Ecology; China Academy of Sciences; Shanghai 200032; 3 Institute of Crop Breeding and Cultivation; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-07-10 Published:2003-07-10

摘要: 利用伽玛射线对粳稻品种中花11进行辐照,以诱导广泛的形态突变体,为水稻功能基因组研究提供基础材料。辐射第1代单本移栽并实行单株收获;辐射第2代(M2)播种1万个家系,自苗期开始至成熟期各个生育阶段从中独立选获各类形态突变体540个;经M3复选获得包括叶、茎、穗和育性变化等各类突变体431份。统计结果表明,形态性状的突变频率为5.1%。关键词

关键词: 水稻, 辐射, 突变体, 形态性状

Abstract: The japonica rice (Oryza sativa L.) variety Zhonghua 11, developed by anther culture technology, was irradiated by gamma rays to induce extensive mutation for functional genomics research use. M 1 generation was transplanted and harvested by each single plant. Ten thousand families of M 2 generation were planted. From seedling stage to maturity stage in M2 generation, 540 morphological mutants were isolated. In M3 generation 431 mutants including leaf, stalk, panicle and sterility variations were identified and selected repeatedly. The statistic analysis indicated that the mutation frequency of morphological character was 5.1%.

Key words: Oryza sativa, irradiation, mutant, morphological characteristics