中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻花器介导法转目标基因的研究

李小湘; 何迎春; 高必达   

  1. 1湖南省水稻研究所， 湖南 长沙 410125； 2湖南农业大学 植物科技学院， 湖南 长沙 410128
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-07-10 发布日期:2003-07-10

Studies on Target Genes Transferred by Rice Floral Organ-Mediated Method

LI Xiao-xiang 1; HE Ying-chun 2; GAO Bi-da 2   

  1. （1 Hunan Rice Research Institute; Hunan Academy of Agricultural Sciences; Changsha 410125; China; 2College of Plant Science and Technology; Hunan Agricultural University; Changsha 410128; China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-07-10 Published:2003-07-10

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被引次数

4

摘要： 研究和分析了受体品种、质粒浓度对水稻花器介导法转化的影响。供试的20个水稻品种（系）中有9个适用于质粒转化,不同受体品种要求的质粒适宜浓度不同。经潮霉素（hygromycin）筛选、PCR分析、抗稻纹枯病和稻瘟病鉴定、GUS活性检测转基因水稻后代，验证了水稻花器介导法转目标基因的有效性。获得了含细菌几丁质酶基因的优丰162转基因T4 代，它表现出对叶瘟病和纹枯病抗性有明显提高。

关键词: 水稻, 转基因, 几丁质酶基因, 抗病性

Abstract: Effects of receptor varieties, plasmids concentrations on the rice transformation rate via floral organ-mediated method were studied. Of 20 tested rice varieties, nine varieties were suitable for transformation. The feasible plasmids concentrations for rice transformation varied with receptor varieties in a rather wide range of concentrations. The validity of target genes transferred by rice floral organ-mediated method were verified by PCR analysis, evaluation of resistance to sheath blight and blast, screening by hygromycin B and examination of GUS activity in transgenic rice. T4 transgenic rice plants containing Serratia marcescens chitinase gene showed resistance to rice sheath blight and rice leaf blast after inoculation with pathogens.

Key words: rice, gene transfer, chitinase gene, disease resistance