中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻粳型亲籼系S-b座位基因型鉴定

丁效华; 张泽民; 曾瑞珍; 李文涛; 张桂权*   

  1. 华南农业大学 植物分子育种研究中心， 广东 广州 510642
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-10-10 发布日期:2003-10-10

Genotypic Identification of the S-b Locus in the Ind ica-Compatible Japonica Lines of Rice (Oryza sativa)

DING Xiao-hua; ZHANG Ze-min; ZENG Rui-zhen; LI Wen-tao; ZHANG Gui-quan   

  1. （Plant Molecular Breeding Research Center; South China Agricul tural University; Guangzhou 510642; China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-10-10 Published:2003-10-10
  • Contact: ZHANG Gui-quan

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摘要： S-b是控制水稻籼粳亚种间杂种不育性的基因座位之一。在该座位，台中65的基因型为Sj/Sj，广陆矮4号的基因型为Si/Si。以台中65和广陆矮4号为遗传测验种，分别与4个粳型亲籼系配组，根据紧密连锁分子标记基因型在这些组合F2群体中的偏态分离程度， 测定了这4个粳型亲籼系在F1花粉不育性S-b基因座位的基因型，结果表明，G2416-3和G2417-2-1在S-b基因座位的基因型为Si/Si，而G2605和G3004-4的基因型为Sn/Sn。比较了不同粳型亲籼系S-b基因座位等位基因遗传效应的大小，结果表明G2416-3携带的Si基因与G2417-2-1携带的Si基因之间，以及G2605携带的Sn基因与G30044携带的Sn基因之间没有显著差异，而Si 与Sn之间遗传效应差异极显著。

关键词: 水稻, 粳型亲籼系, 花粉不育性, 复等位基因, 微卫星标记

Abstract: S-b is one of the hybrid sterility loci between indica and japonica cultivars in rice. The genotype of Taichung 65 (T65) and Guanglu’ai 4 (GLA 4) at the locus is Sj/Sj and Si/Si, respectively. Four indica-compati ble japonica lines (ICJLs), G2416-3, G2417-2-1, G2605 and G3004-4 were cros sed with testers, T65 and GLA 4, and their S-b locus genotypes were identified. The segregation of PCR-based molecular markers linked tightly with S-b locus in F2 population showed that G2416-3 and G2417-2-1 possessed Si/Si genotype, while G2605 and G3004-4 contained genotype Sn/Sn. Comparison of genetic effect among the alleles at S-b locus in four ICJLs showed no significant difference between the Si genes in G241 6-3 and G2417-2-1, and between the Sn genes in G2605 and G3004-4, but significant difference between the Si and Sn genes.

Key words: rice, indica-compatible japonica lines, pollen ster ility, multi-alleles, microsatellite marker