中国水稻科学

中国水稻科学

• 研究报告 •    下一篇

模块表达序列标签技术的建立及相关水稻cDNA阵列的应用

庄晓峰; 李德葆*; Cabezas DANIEL; 戴承恩; 娄沂春; 董海   

  1. 浙江大学 农业与生物技术学院 生物信息与基因网络研究组， 浙江 杭州 310029； *通讯联系人
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-01-10 发布日期:2004-01-10

Establishment of Module-ESTs System aod Application of cDNA Microarray from Rice

ZHUANG Xiao-feng; LI De-bao*; Cabezas DANIEL; DAI Cheng-en; LOU Yi-chun; DONG Hai-tao   

  1. （Bioinformatics and Gene Network Research Group; College of Agriculture and Biotechnology; Zhejiang University; Hangzhou 310029; China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-01-10 Published:2004-01-10

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摘要： 提出了一种新的表达序列标签制作技术——模块表达序列标签（MEST）技术，利用该技术短时间内分离完成1985条水稻cDNA片段，制备完成相应的水稻cDNA阵列。实验证实：该技术体系能成功分离目的基因，正确检测基因表达丰度的改变，并具备通过对基因群体表达模式的监测以实现对生物学事件的精确描述。M-EST概念是对EST技术的完善和重要补充，运用M-EST技术能有效利用生物信息学知识，加快基因克隆速率，为基因功能的研究提供手段和工具。关键词:

关键词: 蛋白模体, 蛋白模块, 表达序列标签, cDNA阵列, 水稻

Abstract: Module Expressed Sequence Tag (M-EST), a new technology for the research of EST, was described. A cDNA array was spotted out, containing 1985 cDNA fragments that were obtained by using the technology of M-EST. Results showed that the technology was very useful to isolate the target gene, to inspect the expression profile and to investigate the interaction between biological phenotype and expression pattern of gene society. According to the results, it is deemed that M-EST was an important complement and perfection of the concept of EST. It will be an effective measure for using the know-ledge of bioinformatics to improve the velocity of gene isolation and to provide tools for the function research.

Key words: motif, module, expressed sequence tag, cDNA array, rice