中国水稻科学

中国水稻科学

• 综述与专论 • 上一篇    下一篇

水稻基因组测序和分析的研究进展

郭龙彪1,2; 程式华1,*; 钱前1,*   

  1. 1中国水稻研究所 水稻生物学国家重点实验室， 浙江 杭州 310006; 2 浙江大学 生命科学学院， 浙江 杭州 310029； *通讯联系人， E-mail:shcheng@mail.hz.zj.cn； qianqian188@hotmail.com
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-11-10 发布日期:2004-11-10

Highlights in Sequencing and Analysis of Rice Genome

GUO Long-biao1; 2; CHENG Shi-hua1*; QIAN Qian1*   

  1. 1State Key Laboratory of Rice Biology; China National Rice Research Institute; Hangzhou 310006; China; 2College of Life Science; Zheji ang University; Hangzhou 310029; China
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-11-10 Published:2004-11-10

PDF

被引次数

49

摘要： 综述了水稻基因组测序的工作进展和水稻基因组信息。至2002年，籼、粳稻两个亚种基因组工作“框架图”的测定及粳稻基因组全长序列的精确测定已相继完成。初步得到了水稻非冗余序列389.81~409.76 Mb；预测水稻基因总数达32 000~56 000个，少于30%的基因被功能分类；基因内GC含量梯度明显；外显子变异少、内含子变化大；籼稻和粳稻的序列差异达22%以上；水稻与其他禾谷类基因之间有广泛的共线性，但与拟南芥的共线性是有限的。基因组测序不仅开辟了基因组学这一生命科学的新兴学科，而且带动了生物信息学、蛋白质组学等一批新兴学科和技术的发展。

关键词: 水稻, 基因组测序, 基因组信息

Abstract: A highlight of rice genome sequencing and its information were presented. Till 2002 two working drafts for rice subspecies indic a and japonica genomes and a high-quality draft sequence for the japonica had been produced. The initial assembly of the non-redundant sequences reach ed 389.81-409.76 Mb and there are around 32 000-56 000 genes. Less than 30% of them have been annotated. There is a significant gradient in GC contents within a gene. Exon sizes are narrowly constrained, but intron sizes can be highly variable within and between organisms. The genome sequence differences between i ndica and japonica rice are over 22%. Nearly half of the predicted rice genes have no obvious homologs with Arabidopsis although there is substantial homology with available expressed sequence tags (ESTs) in other c ereals. Genome sequencing not only opened a new era for the life research-genom ics, but also promoted the development of other subjects, such as bioinformatics and proteomics.

Key words: rice, genome sequencing, genomic information