中国水稻科学

中国水稻科学

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转Xa21基因不育系皖21A的白叶枯病抗性与利用

倪大虎1; 杨剑波1,*; 吴家道1; 李莉1; 易成新1; 许传万1; 汪秀峰1; 贾士荣2; 张世平3   

  1. 1安徽省农业科学院 水稻研究所， 安徽 合肥 230031； 2中国农业科学院 生物技术研究所， 北京 100081； 3International Laboratory for Tropical Agricultural Biotechnology, The Scripps Research Institute, La Jolla, CA92037, USA; *通讯联系人
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-11-10 发布日期:2004-11-10

Resistance to Bacterial Blight and Utilization of Xa21-Transgenic Sterile Line Wan 21A

NI Da-hu1; YANG Jian-bo 1*; WU Jia-dao1; LI Li1; YI Chen-xin1; XU Chuan-wan 1; WANG Xiu-feng 1; JIA Shi-rong 2; ZHANG Shi-ping3   

  1. 1Rice Research Institute; Anhui Academy of Agricultural Sciences; Hefei 230031; China; 2Biotechnology Research Institute; Chines e Academy of Agricultural Sciences; Beijing 100081; 3Internationa l Laboratory for Tropical Agricultural Biotechnology; The Scripps Research Insti tute; La Jolla; CA92037; USA;
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-11-10 Published:2004-11-10

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被引次数

41

摘要： 利用基因枪转化法将Xa21基因导入80-4B（BT型不育系80-4A的保持系），选育出纯合稳定的株系皖21B。以80-4A与皖21B连续回交转育，育成了高抗白叶枯病的不育系皖21A，对皖21A和皖21B的不同世代材料进行PCR分析和田间白叶枯病菌抗性试验，表明Xa21基因稳定遗传且抗性稳定。皖21A与恢复系R18、HP121、U160配制杂交组合具有良好的抗病性和产量潜力。

关键词: 转基因, 水稻, 白叶枯病, 抗性, 不育系

Abstract: The stable transgenic rice Wan 21B with Xa21 gene conferring the resistance to rice bacterial blight was obtained with the microbombardment method. Wan 21B w as used to produce homogeneous sterile line Wan 21A by using backcross with 80 -4A. Xa21 gene was confirmed i n different generations of Wan 21A and Wan 21B through PCR assay, and the offsp ring possessed the stable resistance to bacterial blight according to the expe riment results of inoculation in the field. The hybrids of Wan 21A and three d ifferent elite restorer lines displayed good agronomic characters and high y ield potentiality.

Key words: gene transfer, rice, bacterial blight, resistance, s terile line