中国水稻科学

中国水稻科学

• 研究报告 •    下一篇

鉴定水稻品种的微卫星标记筛选

施勇烽;应杰政;王磊; 朱智伟;庄杰云   

  1. 1 中国水稻研究所 农业部稻米及制品质量监督检验测试中心， 浙江 杭州 310006； 2 中国水稻研究所 国家水稻改良中心/水稻生物学国家重点实验室， 浙江 杭州 310006； 3 中国水稻研究所 科技信息中心， 浙江 杭州 310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-05-10 发布日期:2005-05-10

Screening SSR Markers for Rice Variety Identification

SHI Yong-feng , YING Jie-zheng , WANG Lei , ZHU Zhi-Wei , ZHUANG Jie-yun
  

  • Received:1900-01-01 Revised:1900-01-01 Online:2005-05-10 Published:2005-05-10

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被引次数

186

摘要： 选用58个水稻微卫星标记，对目前应用较广的28份杂交水稻亲本材料进行多态性分析，并比较了其中14个标记应用3.0%琼脂糖凝胶电泳和6.0%非变性聚丙烯酰胺凝胶电泳的检测结果。研究表明，所应用的标记可将所有供试水稻材料区分开，不同标记的多态性检测能力差异较大；两种检测方法所得结果高度一致，但非变性聚丙烯酰胺凝胶电泳分辨率较高、带型清晰，能更准确反映水稻材料间的差异。对于一般水稻材料而言，选用12个标记可将不同材料区别开的几率大于99.9%，但要区分遗传相似性较高的材料，则可能需挑选高多态性标记和（或）增加检测的标记数。

关键词: 水稻, 微卫星标记, 非变性聚丙烯酰胺凝胶电泳, 琼脂糖凝胶电泳, 品种鉴定

Abstract: A total of 58 DNA simple sequence repeat (SSR) markers were selected to detect polymorphisms among 28 parental lines of commercial F 1 hybrid rice used in China. Fourteen of the markers were assayed by using both agarose gel electrophoresis and non-denaturing polyacrylamide gel electrophosresis (PAGE). It was shown that each of the rice lines can be unambiguous distinguished, and great variations on the frequency of polymorphism were observed among the markers used. Similar results were generated by agarose gel electrophoresis and non-denaturing PAGE, while the non-denaturing PAGE had higher resolution, showing feasibility for its application in the variety identification of rice. On average, the probability of distinguishing the rice lines by detection with 12 SSR markers was higher than 99.9%, but markers of higher variation and/or more markers may be required for varieties having high genetic similarity.

Key words: rice, simple sequence repeat, non-denaturing polyacrylamide gel electrophoresis, agarose gel electrophoresis, variety identification