中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻Wx复等位基因的鉴定及单片段代换系的建立

曾瑞珍;张泽民;何风华;席章营;Akshay TALUKDAR;施军琼;秦利军;黄朝锋;张桂权*   

  1. 华南农业大学 广东省植物分子育种重点实验室， 广东 广州 510642
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-11-10 发布日期:2005-11-10

Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines in Rice

ZENG Rui-zhen , ZHANG Ze-min , HE Feng-hua , XI Zhang-ying , Akshay TALUKDAR , SHI Jun-qiong , QIN Li-jun , HUANG Chao-feng , ZHANG Gui-quan   

  • Received:1900-01-01 Revised:1900-01-01 Online:2005-11-10 Published:2005-11-10

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摘要： 利用微卫星标记“484/485”和“484/W2R”结合AccⅠ酶切对278份来自国内外水稻品种（系）进行了Wx座位复等位基因分析，共检测到15种等位基因，其中(CT)12-G、(CT)15-G、(CT)16-G、(CT)17-G、(CT)18-G、(CT)21-G为新发现的等位基因。以含不同Wx等位基因的20个品种（系）为供体亲本，利用回交和微卫星标记辅助选择相结合的方法，建立了72个以Wx等位基因为 (CT)11-G 的“华粳籼74”为受体的Wx复等位基因单片段代换系。这些单片段代换系共包含12种Wx等位基因，其代换片段长度最短为2.2 cM，最长为77.3 cM，平均为17.4 cM。

关键词: 水稻, 糯性基因, 单片段代换系

Abstract: The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by the use of microsatellite class and G-T polymorphism. Among them, CT 12-G, CT 15-G, CT 16-G, CT 17-G, CT 18-G and CT 21-G are new ones. Seventy-two single segment substitution lines (SSSLs) carrying different alleles at the Wx locus were developed by the use of Huajingxian 74 with the CT 11-G allele as a recipient and 20 varieties containing 12 alleles at the Wx locus as donors. The estimated length of the substituted segments ranged from 2.2 cM to 77.3 cM with an average of 17.4 cM.

Key words: rice, waxy gene, single segment substitution line