中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

转溶菌酶基因水稻回交转育籼型杂交稻亲本

易自力1,*;王紫萱1;覃静萍1;蒋建雄1;谭炎宁1;周清明2   

  1. 1湖南农业大学 细胞工程重点实验室, 湖南 长沙 410128; 2湖南农业大学 农学院， 湖南 长沙 410128
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-03-10 发布日期:2006-03-10

Transfer of Lysozyme Gene into indica Parents of Hybrid Rice by Backcrossing

YI Zi-li , WANG Zi-xuan , QIN Jing-ping , JIANG Jian-xiong , TAN Yan-ning , ZHOU Qing-ming   

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-03-10 Published:2006-03-10

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被引次数

33

摘要： 以抗稻瘟病的粳稻中花9号转溶菌酶基因材料D212与籼型杂交稻两优培九的恢复系9311及不育系培矮64S、汕优63的恢复系明恢63分别杂交和多代回交，进行外源溶菌酶基因的转育。对获得的转育回交后代B39311、B3MH63、B2PA64S和回交自交后代B2F29311、B2F2MH63、B1F2PA64S进行了PCR分析，表明外源基因在回交后代中呈1∶1分离，而在回交的自交后代中呈3∶1分离，证明外源溶菌酶基因是以单拷贝稳定传递给后代的。2003年和2004年对转育后代和测交组合进行了稻瘟病抗性调查，结果表明，转育后代抗性与轮回亲本相比、测交组合抗性与对应杂交稻组合相比都有了较大提高。随着转育回交世代的增加，抗性增强得越明显。研究表明通过回交转育方法将外源溶菌酶基因导入杂交稻亲本是选育抗稻瘟病杂交稻新组合的一条简便有效的途径。

关键词: 杂交稻, 溶菌酶基因, 稻瘟病, 抗性, 回交, 育种

Abstract: A lysozyme gene resistant to rice blast from the donor D2-1-2, a transgenic japonica rice line of Zhonghua 9, was transferred into the sterile line Pei′ai 64S(PA64S) and restorer line 9311 of the hybrid Liangyoupeijiu, and the restorer line Minghui 63 (MH63) of Shanyou 63 by successive backcrossing. PCR analysis confirmed that foreign lysozyme gene was segregated with the ratio of 1∶1 in backcross generations of B39311, B3MH63 and B2PA64S, and with the ratio of 3∶1 in inbred generations of B2F29311, B2F2MH63 and B1F2PA64S, indicating the foreign gene was stably inherited over successive generations as a dominant single copy gene. The resistance to rice blast of the backcross or inbred generations and corresponding testcross combinations were investigated in the years of 2003 and 2004, and the results showed that their resistance to rice blast had a greater improvement than that of the corresponding recurrent parents or the corresponding hybrid combinations, respectively. The resistance to rice blast became stronger with the generations of the transferred rice progressed. This confirmed that transferring the lysozyme gene into hybrid parents by backcrossing was a simple and effective approach to develope new hybrid combinations resistant to rice blast.

Key words: hybrid rice, lysozyme gene, rice blast, resistance, backcrossing, breeding