水稻叶片自然衰老过程中Rubisco大亚基的含量变化

doi:10.3969/j.issn.10017216.2009.05.17

摘要/Abstract

摘要： 以水稻低叶绿素b突变体及其野生型（镇恢249）为材料，研究了水稻第5叶叶片自然衰老过程中光合放氧、Rubisco大亚基含量变化以及对胰蛋白酶敏感性的变化，并通过喷施不同的抗氧化剂以及氧化剂研究其对Rubisco大亚基含量变化的影响。结果表明，叶片全展后随着衰老进程，突变体与野生型光合放氧速率、Rubisco大亚基相对含量呈下降趋势，抗氧化剂可以不同程度地缓解Rubisco大亚基含量的下降，而氧化剂则加速Rubisco大亚基含量的下降。低叶绿素b突变体的光合放氧速率高于野生型，Rubisco大亚基含量下降慢于野生型，并且衰老进程中突变体Rubisco大亚基对胰蛋白酶的敏感性低于野生型，推测活性氧是Rubisco降解的调节剂之一。

关键词: 水稻, 叶绿素b, 突变体, 衰老, 光合放氧, 1, 5二磷酸核酮糖羧化酶/加氧酶

Abstract: A rice mutant with low chlorophyll b and its wild type rice were adopted to investigate the changes in photosynthetic oxygen evolution rate, and content and sensitivity to trypsin proteolysis of Rubisco large subunit (LSU) during leaf senescence, as well as the effects of antioxidant and oxidant on changes in relative content of Rubisco LSU. The results showed that after full expansion of leaves in the mutant and its wild type plants, oxygen evolution and relative content of Rubisco LSU declined significantly. The degradation of Rubisco LSU could be retarded by antioxidants (reduced glutathione and ascorbic acid) but accelerated by oxidants (oxidized glutathione and H2O2). The oxygen evolution rate in the mutant was higher than that in the wild type. On the contrary, the degradation of Rubisco LSU was slower in the mutant than in the wild type as leaf senescence. Lower sensitivity to trypsin proteolysis of Rubisco LSU was found in the mutant in comparison with the wild type. It is suggested that reactive oxygen species (ROS) mediate the degradation of Rubisco LSU.

Key words: rice, chlorophyll b, mutant, senescence, oxygen evolution, rubulose1, 5bisphophate carboxylase

李瑞,周玮,李丽,陆巍. 水稻叶片自然衰老过程中Rubisco大亚基的含量变化
[J]. 中国水稻科学 2009,23(5): 555-558 . , DOI: 10.3969/j.issn.10017216.2009.05.17 .

LI Rui,ZHOU Wei,LI Li,LU Wei*. Changes in Relative Content of Rubisco Large Subunit in Naturally Senescing Rice Leaves[J]. Chinese Journal of Rice Science 2009,23(5): 555-558 ., DOI: 10.3969/j.issn.10017216.2009.05.17 .

参考文献

[1]Jensen R G. Activation of Rubisco controls CO2 assimilation in light: A perspective on its discovery. Photosyn Res, 2004, 82: 187-193.

[2]Spreizter R J, Salvucci M E. Rubisco: Structure, regulatory interactions and possibilities for a better enzyme. Annu Rev Plant Biol, 2002, 53: 449-475.

[3]Maeda N, Kanai T, Atomi H, Imanaka T. The unique pentagonal structure of an archaeal Rubisco is essential for its high thermostability. J Biol Chem, 2002, 277(35): 31656-31662.

[4]Xu D Q. Progress in photosynthesis research: From molecular mechanisms to green revolution. Acta Phytophysiol Sin, 2001, 27(2): 97-108.

[5]Peoples M B, Beilharz V C, Waters S P, et al. Nitrogen redistribution during grain growth in wheat (Triticum aestivum L.). Planta, 1980, 149: 241-251.

[6]彭新湘, 彭少兵. 水稻叶片在自然衰老过程中1, 5二磷酸核酮糖羧化酶/加氧酶的降解. 植物生理学报, 2000, 26(1): 46-52

[7]Tomimatsu Y, Donovan J W. Effect of pH, Mg2+, CO2 and mercurials on the circular dichroism, thermal stability and light scattering of ribulose 1,5bisphosphate carboxylases from alfalfa, spinach and tobacco. Plant Physiol, 1981, 68: 808-813.

[8]Li G F, Mao H B, Ruan X, et al. Association of heatinduced conformational change with activity loss of Rubisco. Biochem Biophys Res Comm, 2002, 290: 1128-1132.

[9]Dann M S, Pell E J. Decline of activity and quantity of ribulose bisphosphate carboxylase oxygenase and net photosynthesis in ozonetreated potato foliage. Plant Physiol, 1989, 91: 427-432.

[10]Eckardt N A, Pell E J. Oxidative modification of Rubisco from potato foliage in response to ozone. Plant Physiol Biochem, 1995, 33: 273-282.

[11]Mehta R A, Fawcett T W, Porath D, et al. Oxidative stress causes rapid membrane translocation and in vivo degradation of ribulose1,5bisphosphate carboxylase/oxygenase. J Biol Chem, 1992, 267: 2810-2816.

[12] 陆巍, 李森, 李国富，等. H2O2对水稻Rubisco稳定性的影响. 植物生理学通讯, 2002, 38(3): 213-216.

[13]Li S, Lu W, Li G F, Gong Y D, et al. Interaction of hydrogen peroxide with ribulose1,5bisphosphate carboxylase/oxygenase from rice. Biochemistry(Moscow), 2004, 69: 1136-1142.

[14] 戴新宾, 曹树青, 许晓明，等. 低叶绿素b高产水稻突变体及其光合特性的研究. 植物学报， 2000， 42（2）： 12891294.

[15] 许晓明, 张荣铣, 唐运来. 低叶绿素含量对水稻突变体吸收光能分配特性的影响. 中国农业科学， 2004， 37（3）： 339-343.

[16] 张荣铣, 许晓明, 戴新宾, 等. 叶绿素b含量低的水稻突变体的光合功能衰退及其与活性氧的关系. 植物生理与分子生物学学报, 2003, 29: 104-108.

[17] Rosenvasser S, Mayak S, Friedman H. Increase in reactive oxygen species(ROS) and in senescenceassociated gene transcript(SAG) levels during darkinduced senescence of Pelargonium cuttings, and the effect of gibberellic acid. Plant Sci, 2006, 170: 873-879.

[18] 周玮, 刘文真, 张荣铣, 等. 水稻TDNA插入突变体库中Rubisco抗氧化胁迫株系的筛选. 作物学报, 2007, 33(12): 2063-2066.

[19]Desimone M, Henke A, Wagner E. Oxidative stress induces partial degradation of the large subunit of ribulose1,5bisphosphate carboxylase/oxygenase in isolated chloroplasts of barley. Plant Physiol, 1996, 111: 789-796.

[20]Ishida H, Shimizu S, Makino A, et al. Lightdependent fragmentation of the large subunit of ribulose1,5bisphosphate carboxylase/oxygenase in chloroplasts isolated from wheat leaves. Planta, 1998, 204: 305-309.

[21]Nakano R, Ishida H, Makino A, et al. In vivo fragmentation of the large subunit of ribulose1,5bisphosphate carboxylase by reactive oxygen species in an intact leaf of cucumber under chillinglight conditions. Plant Cell Physiol, 2006, 47: 270-276.

[22] Moreno J, GarcíaMurria M J, MarínNavarro J. Redox modulation of Rubisco conformation and activity through its cysteine residues. J Exp Bot, 2008, 59(7): 1605-1614.