Changes in Relative Content of Rubisco Large Subunit in Naturally Senescing Rice Leaves
LI Rui, ZHOU Wei, LI Li, LU Wei*
Department of Plant Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China； *Corresponding author， E-mail: luw@njau.edu.cn
Abstract：
A rice mutant with low chlorophyll b and its wild type rice were adopted to investigate the changes in photosynthetic oxygen evolution rate, and content and sensitivity to trypsin proteolysis of Rubisco large subunit (LSU) during leaf senescence, as well as the effects of antioxidant and oxidant on changes in relative content of Rubisco LSU. The results showed that after full expansion of leaves in the mutant and its wild type plants, oxygen evolution and relative content of Rubisco LSU declined significantly. The degradation of Rubisco LSU could be retarded by antioxidants (reduced glutathione and ascorbic acid) but accelerated by oxidants (oxidized glutathione and H2O2). The oxygen evolution rate in the mutant was higher than that in the wild type. On the contrary, the degradation of Rubisco LSU was slower in the mutant than in the wild type as leaf senescence. Lower sensitivity to trypsin proteolysis of Rubisco LSU was found in the mutant in comparison with the wild type. It is suggested that reactive oxygen species (ROS) mediate the degradation of Rubisco LSU.
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2009, Vol. 23 
