中国水稻科学

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空气中CO2浓度升高条件下水稻抽穗期的QTL定位

范桂枝1,4 ;李军营1 ;王春明2 ; 谢辉1; 徐长亮1 ;朱建国3; 万建民2; 蔡庆生1,*   

  1. 1南京农业大学 生命科学学院, 江苏 南京 210095; 2南京农业大学 作物遗传与种质创新国家重点实验室, 江苏 南京 210095; 3中国科学院 南京土壤研究所, 江苏 南京 210008; 4东北林业大学 生命科学学院, 黑龙江 哈尔滨 150040; *通讯联系人, E-mail: qscai@njau.edu.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-05-10 发布日期:2006-05-10

QTL Analysis for Heading Date of Rice under Free Air CO2 Enrichment Conditions

FAN Gui-zhi , LI Jun-ying , WANG Chun-ming , XIE Hui , XU Chang-liang , ZHU Jian-guo , WAN Jian-min , CAI Qing-sheng   

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10

摘要: 以65个水稻染色体片段置换系(chromosome segment substitution lines,CSSLs)为材料,对比分析了正常大气CO2浓度(对照)和开放式空气CO2浓度升高(free air CO2 enrichment,FACE,大气CO2浓度增加200 μmol/mol)下水稻抽穗期的变化,并定位了相关QTL(quantitative trait loci)。供试株系的抽穗期对CO2浓度升高表现为提早、延迟和不变等3种响应,抽穗期两极变化最大的株系为AI46(提前11 d)和AI63(延迟6 d)。两种条件下,共检测到9个控制抽穗期的QTL,分布在第3、4、6、7、8、10和11染色体上。其中位于第6和第8染色体上的 qHD64和 qHD84在两种CO2浓度下都检测到,但在FACE下的贡献率均显著增大;而 qHD3A3和 qHD11A7只在正常条件下检测到, qHD4F4、 qHD10F4和 qHD11F4则只在FACE下检测到。 暗示控制水稻抽穗期的基因表达易受环境CO2浓度的影响。

关键词: CO2浓度, 水稻, 染色体片段置换系, 抽穗期, 数量性状座位

Abstract: A mapping population consisting of 65 chromosome segment substitution lines(CSSLs) was used to detect quantitative trait loci (QTL) for heading date of rice !(Oryza sativa L .) under the ambient atmosphere CO2 concentration (Ambient) and free air CO2 enrichment (FACE, ambient + 200 μmol/mol) conditions. Both positive and negative responses to elevated CO2 at heading date of CSSLs were found. Among them, two CSSLs, AI46 (advanced 11 days) and AI63 (delayed 6 days), were the most sensitive to elevated CO2 in different directions. A total of 9 QTLs for heading date were detected on chromosomes 3, 4, 6, 7, 8, 10 and 11 under the FACE and Ambient. Of them, qHD6-4 and qHD8-4 were simultaneously detected under both the FACE and Ambient, but the percentage of variation explained under the FACE was much higher than that under the Ambient, in addition, two QTLs, qHD3 A-3 and qHD11 A-7 were only detected under Ambient, and three QTLs, qHD4 F-4 , qHD10 F-4 and qHD11 F-4 only under FACE, suggesting that the genes affecting heading date were sensitive to atmosphere CO2 concentration.

Key words: CO2 concentration, rice, chromosome segment substitution lines, heading date, quantitative trait locus