中国水稻科学

• 研究报告 •    下一篇

水稻成熟胚离体培养有关性状的遗传分析

陈 璋;朱秀英   

  1. 福建农学院作物遗传育种研究所,福州350002
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1991-12-05 发布日期:1991-12-05

Inheritance in vitro Response of Mature Embryo Culture in Oryza sativa

Chen Zhang; Zhu Xiuying   

  • Received:1900-01-01 Revised:1900-01-01 Online:1991-12-05 Published:1991-12-05

摘要: 采用p(p+1)/2半完全双列杂交与f×m 不完全双列杂交法,研究了两个水稻群体成熟胚离体培养的遗传表现。结果表明,水稻成熟胚愈伤组织诱导率和绿苗分化率的高低主要受亲本基因型的影响,即基因型不同,差异很大,不仅存在基因的加性效应,也表现出非等位基因的互作效应,遗传表现为部分显性,其遗传模型为加性一显性一上位性模型,遗传力较低,而成熟胚的离体培养力的遗传模型为加性一显性模型,具较高的遗传力。

关键词: 水稻, 成熟胚, 离体培养, 双列分析, 遗传方差, 配合力, 遗传力

Abstract: The study of genetic model, combining ability and heritability of callus induction rate, green plant differentiation rate and culturability of rice embryo culture was made with both a p(p+1)/2 diallel set of crosses (desigh I) and f×m incomplete diallel set of crosses (design Ⅱ) in 1989. Variance analysis indicated the significant difference existed in genotypes. The genotype was of a mainly factor affecting the culturability in vitro, and differed among the various traits on genotype influence. Wr-Vr graph showed all the traits appeared partly dominant. The variance of GCA in female and SCA were highly significant by F-test for the overwhelming majority of characters. The effect of female on F1 hybrids in the culturability in vitro was significantly. The GCA estimates showed that there are difference between parents for the same traits, as well as between various characters of a parent. Moreover, SCA varied with combinations. Callus induction rate and green plant differentiation rate of embryo in vitro culture were controlled by additive-dominance-epistasis model in the inheritance, and culturability in vitro by additive-dominance model without epistasis in its inheritance.

Key words: Oryza sativa, In vitro mature embryo culture, Diallel analysis, Genetic variance, Combining ability, Heritability